Details

Microbial Enzymes


Microbial Enzymes

Production, Purification, and Industrial Applications, 2 Volume Set
1. Aufl.

von: Dinesh Yadav, Pankaj Chowdhary, Gautam Anand, Rajarshi Kumar Gaur

295,99 €

Verlag: Wiley-VCH (D)
Format: PDF
Veröffentl.: 09.10.2024
ISBN/EAN: 9783527844357
Sprache: englisch
Anzahl Seiten: 848

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Beschreibungen

<p><b>Comprehensive discussion of production and purification strategies for microbial enzymes important to various industries, from food and beverages to pharmaceuticals</b> <p><i>Microbial Enzymes</i> provides expert insight into diverse aspects of microbial enzymes, highlighting strategies for their production, purification, and manipulation, elucidating eco-friendly industrial applications, and discussing several production processes, such as the production of cellulose and non-synthetic indigo dye. This book emphasizes recent technological interventions in microbial enzyme technology like metagenomics, system biology, molecular biology, genomics, directed evolution, and bioinformatics. <p>The important microbial enzymes highlighted in this book include xylanases, ureases, methane monooxygenase, polyhydroxyalkanoates, pectinases, peroxidases, α-L-rhamnosidase, alkane hydroxylases, laccases, proteases, gallic acid decarboxylase, chitinases, beta-glucosidase, lipases, inulinases, tannase, mycozyme, ACC deaminase, ligninolytic enzymes, and many more. <p>Novel treatment methods involving strains of microorganisms with desirable properties applicable in the process of bioremediation through mitigating climate concern, increasing green production technology, improving agriculture productivity, and providing a means of earning a livelihood are discussed. Readers will also gain state-of-the-art background knowledge on existing technologies and their current challenges and future prospects. <p>Contributed to by leading experts in the field and edited by four highly qualified academics, <i>Microbial Enzymes</i> explores important topics including: <ul><li>Strategies for the discovery and enhancement of enzyme function, and potentials of system biology to better understand the kinetics of industrially important enzymes</li><li>Production and therapeutic applications of monoclonal antibodies in cancer and other diseases, and characterization of tannase as a virulence factor</li><li>Opportunities to produce enzymes through food waste and byproducts, and recent developments in computational tools</li><li>Use of Omics tools in the discovery of fungal enzymes and secondary metabolites</li></ul> <p><i>Microbial Enzymes</i> is a thorough and highly practical reference on the subject for students, scientists, biotechnologists, microbiologists, and policymakers working in environmental microbiology, biotechnology, and environmental sciences.
<p><b>Volume 1</b></p> <p>About the Editors xix</p> <p>Preface xxiii</p> <p><b>1 Xylanases: Sources, Production, and Purification Strategies 1<br /> </b><i>Mariana Delgado-Garcia, Lizeth G. Campos-Muzquiz, Rocio G. Castillo-Godina, Sendar D. Nery-Flores, Lissethe Palomo-Ligas, Adriana C. Flores-Gallegos, Beatriz del C. Cutiño-Laguna, and Raul Rodriguez-Herrera</i></p> <p>1.1 Introduction 1</p> <p>1.2 Sources, Production, and Purification Strategies 3</p> <p>1.3 Structure 5</p> <p>1.4 Xylanases as Biocatalyst 8</p> <p>1.5 Genomics Studies on Xylanases 11</p> <p>1.6 Xylanases as a Promising Enzyme for Industrial Applications 13</p> <p>1.7 Industrial Food Applications 14</p> <p>1.8 Future Trends 17</p> <p>1.9 Conclusions 19</p> <p>Acknowledgment 19</p> <p>References 20</p> <p><b>2 Exploration of the Microbial Urease and Their Industrial Applications 31<br /> </b><i>Jackson Khedia, Rupali Gupta, and Gautam Anand</i></p> <p>2.1 Urease Enzyme and Its History 31</p> <p>2.2 Urea Hydrolysis 31</p> <p>2.3 Sources and Molecular Attributes of Urease Enzyme 32</p> <p>2.4 Urease Purification 33</p> <p>2.5 Applications of Urease Enzyme 36</p> <p>2.6 Conclusion and Future Aspects 40</p> <p>References 41</p> <p><b>3 Methane Monooxygenase Production and Its Limitations 47<br /> </b><i>Gaurav S. Rana, Neetika Naudiyal, Vaibhav Badoni, Amit S. Rana, Ashutosh Dubey, and Ashok K. Verma</i></p> <p>3.1 Introduction 47</p> <p>3.2 Classes of MMO 48</p> <p>3.3 Structure and Active Site of MMO 50</p> <p>3.4 Mechanism of Action 52</p> <p>3.5 Regulation of MMO 54</p> <p>3.6 Sources of MMO 54</p> <p>3.7 Genetic Engineering of MMOs 55</p> <p>3.8 MMO Production 57</p> <p>3.9 Applications of MMO and Methanotrophs 58</p> <p>3.10 Limitations in MMO Production 61</p> <p>3.11 Conclusion 61</p> <p>References 62</p> <p><b>4 Polyhydroxyalkanoates: An Eco-sustainable Development Toward a Green World 67<br /> </b><i>Abhijeet W. Singh, Ramendra Soni, Arun K. Pal, Jayant Kashyap, Pooja Tripathi, and Vijay Tripathi</i></p> <p>4.1 Introduction 67</p> <p>4.2 Structure, Classification, and Properties of PHAs 69</p> <p>4.3 Production and Synthesis of PHAs 70</p> <p>4.4 Applications of PHAs in the Health Sector 71</p> <p>4.5 Tissue Engineering 71</p> <p>4.6 Bio-implantation Patches 72</p> <p>4.7 Drug Delivery 72</p> <p>4.8 Surgical Applications 72</p> <p>4.9 Orthopedic Applications 73</p> <p>4.10 Industrial Applications of PHAs 73</p> <p>4.11 Agricultural Applications 75</p> <p>4.12 Conclusion and Future Prospective 76</p> <p>Acknowledgments 77</p> <p>References 78</p> <p><b>5 An Insight into Production Strategies for Microbial Pectinases: An Overview 87<br /> </b><i>Shruti Dwivedi, Gautam Anand, Sangeeta Yadav, and Dinesh Yadav</i></p> <p>5.1 Introduction 87</p> <p>5.2 Microbial Pectinases 88</p> <p>5.3 Microbial Pectinases: Mode of Action and Classifications 89</p> <p>5.4 Sources of Microbial Pectinases 89</p> <p>5.5 Production of Microbial Pectinases 95</p> <p>5.6 Bioreactors-based Production of Microbial Pectinases 96</p> <p>5.7 Response Surface Methodology for Enhancing Production of Microbial Pectinases 99</p> <p>5.8 Purification of Microbial Pectinases 101</p> <p>5.9 Immobilization of Microbial Pectinases 102</p> <p>5.10 Future Prospects and Conclusion 102</p> <p>References 107</p> <p><b>6 Hydrocarbon-degrading Enzymes from Mangrove-associated Fungi and Their Applications 119<br /> </b><i>Mark Gabriel M. Galinato and Adeline Su Yien Ting</i></p> <p>6.1 Introduction 119</p> <p>6.2 Hydrocarbon Pollution 120</p> <p>6.2.1 Hydrocarbons 120</p> <p>6.2.2 Hydrocarbon Pollution and Management 121</p> <p>6.3 Mangrove Environments 123</p> <p>6.4 Mangrove-associated Fungi as Hydrocarbon Degraders 126</p> <p>6.5 Ligninolytic Enzymes from Mangrove-associated Fungi 131</p> <p>6.6 Applications and Future Prospects 135</p> <p>6.7 Conclusion 138</p> <p>References 138</p> <p><b>7 Industrially Important Microbial Enzymes Production and Their Applications 149<br /> </b><i>Gebiru Sinshaw, Abate Ayele, Gamachis Korsa, Gessesse K. Bekele, and Mesfin T. Gemeda</i></p> <p>7.1 Introduction 149</p> <p>7.2 Sources of Industrially Important Microbial Enzymes 150</p> <p>7.3 Application of Microbial Enzymes in Industries 154</p> <p>7.4 Challenges and Future Trends of Microbial Enzymes 163</p> <p>7.5 Conclusion 164</p> <p>List of Abbreviations 164</p> <p>Authors’ Contributions 164</p> <p>Acknowledgments 165</p> <p>References 165</p> <p><b>8 Peroxidases: Role in Bioremediation 173<br /> </b><i>Huda Afreen, Ravi Kant Singh, and Pradeep Kumar</i></p> <p>8.1 Introduction 173</p> <p>8.2 Classification of Peroxidases 176</p> <p>8.3 Applications of Different Peroxidases for Environmental Pollution Management 177</p> <p>8.4 Conclusion 183</p> <p>Acknowledgment 183</p> <p>References 183</p> <p><b>9 Microbial α-L-Rhamnosidase and Its Significance in Therapeutics 189<br /> </b><i>Vinita Yadav</i></p> <p>9.1 Introduction 189</p> <p>9.2 Sources 190</p> <p>9.3 Substrate Specificity and Optimality 190</p> <p>9.4 Isolation of Microbial Strains for Producing α-L-Rhamnosidase Enzyme 196</p> <p>9.5 Assay Method 196</p> <p>9.6 Purification Method 198</p> <p>9.7 Biochemical Properties and Application of α-L-Rhamnosidase 199</p> <p>9.8 Summary 201</p> <p>References 202</p> <p><b>10 The Use of Microbial Enzymes in the Food Industries: A Global Perspective 207<br /> </b><i>Hina Radadiya, Rajesh Patel, and Ramesh Kothari</i></p> <p>10.1 Introduction 207</p> <p>10.2 Global Perspective and Demand for Microbial Enzymes in the Food Industry 208</p> <p>10.3 Production of Industrial Enzymes 209</p> <p>10.4 Approach to Boost Properties of Microbial Enzymes 211</p> <p>10.5 Microbial Enzymes in Food Industries 212</p> <p>10.6 Conclusion and Future Perspectives 219</p> <p>References 220</p> <p><b>11 Alkane Hydroxylases: Sources and Applications 225<br /> </b><i>Sangeeta Negi and Satyapriy Das</i></p> <p>11.1 Introduction 225</p> <p>11.2 Sources of Alkane Hydroxylases 227</p> <p>11.3 Production, Purification, and Characterization of Alkane Hydroxylases 229</p> <p>11.4 Applications of Alkane Hydroxylases 231</p> <p>11.5 Future Prospects 235</p> <p>11.6 Conclusion 236</p> <p>References 236</p> <p><b>12 An Overview of Production of Bacterial and Fungal Laccases and Their Industrial Applications 243<br /> </b><i>Sushil K. Singh, Arya Sahu, Manish S. Rajput, and Nand Lal</i></p> <p>12.1 Introduction 243</p> <p>12.2 Structure of Laccase 245</p> <p>12.3 Mode of Action 247</p> <p>12.4 Sources of Laccase 248</p> <p>12.5 Substrates, Mediators, and Screening of Laccases 250</p> <p>12.6 Production of Bacterial Laccases 251</p> <p>12.7 Production of Fungal Laccases 254</p> <p>12.8 Applications of Laccases 254</p> <p>12.9 Conclusion and Future Scope 260</p> <p>References 260</p> <p><b>13 Magic of Microbial Enzymes: Earthworm’s Gut as a Bioreactor 273<br /> </b><i>Jayanta K. Biswas and Anurupa Banerjee</i></p> <p>13.1 Introduction 273</p> <p>13.2 Classification of Enzymes 274</p> <p>13.3 Earthworms: Intestines of the Earth 275</p> <p>13.4 Earthworms and Their Relation with Microbes and Enzymes 277</p> <p>13.5 Importance of Enzymes Related to Earthworms and the Associated Microorganisms 278</p> <p>13.6 Conclusions and Future Perspectives 283</p> <p>References 284</p> <p><b>14 Proteases from Thermophilic Bacteria: Their Significant Characteristics and Recombinant Production 293<br /> </b><i>Nitin Srivastava, Sumit Kumar, and Sunil K. Khare</i></p> <p>14.1 Introduction 293</p> <p>14.2 Thermophilic Bacteria 294</p> <p>14.3 Thermophilic Proteases 297</p> <p>14.4 Stability of Thermophilic Proteases and Underlying Mechanisms 298</p> <p>14.5 Significance of Thermophilic Proteases 299</p> <p>14.6 Recombinant Thermophilic Protease Production Strategies and Related Challenges 300</p> <p>14.7 Enzyme Engineering Strategies 301</p> <p>14.8 Applications 302</p> <p>14.9 Conclusion and Future Perspectives 303</p> <p>References 304</p> <p><b>15 Mining and Redesigning of Microbial Enzymes for the Degradation of Organophosphorus Pesticides 309<br /> </b><i>Fauzia Parween and Rinkoo D. Gupta</i></p> <p>15.1 Introduction 309</p> <p>15.2 Selection of the Starting Gene 310</p> <p>15.3 DNA Level Processes for the Creation of Gene Library 312</p> <p>15.4 Screening of the Gene Library 315</p> <p>15.5 Characterization of Designed Enzymes 318</p> <p>15.6 Conclusion and Future Perspectives 320</p> <p>List of Abbreviations 321</p> <p>References 321</p> <p><b>16 Use of Omics Tools Toward the Discovery of Fungal Enzymes and Secondary Metabolites 329<br /> </b><i>Neelam A. Kungwani, Simran Dani, and Gunjan Sharma</i></p> <p>16.1 Introduction 329</p> <p>16.2 Biotechnological Applications of Key Fungal Enzymes 330</p> <p>16.3 Biotechnological Potential of Fungal Metabolites 333</p> <p>16.4 In Silico Tools for Fungal Enzymes and Secondary Metabolites Prediction 335</p> <p>16.5 Use of In Silico Tools for the Prediction of Fungal Enzymes and Secondary Metabolites 338</p> <p>16.6 Implications and Limitations of the In Silico Studies in Fungal Biology 339</p> <p>16.7 Conclusions and Prospects 339</p> <p>References 339</p> <p><b>17 Bioprospecting of Microbial Enzymes with Application in Environmental Biotechnology: An Omic Approach 345<br /> </b><i>Maricy R. L. Bonfá, Rodrigo M. Pereira, Francine A. Piubeli, Caio C. A. do Prado, and Lucia R. Grossman</i></p> <p>17.1 Introduction 345</p> <p>17.2 Environmental Biotechnology Areas 347</p> <p>17.3 Microbial Enzymes Applied in Environmental Recovery 352</p> <p>17.4 Microbial Enzymes for Bioproduct Manufacturing 356</p> <p>17.5 Omics Approaches for Bioprospecting Enzymes 360</p> <p>17.6 Conclusion and Perspectives 374</p> <p>References 375</p> <p><b>18 Recent Trends in Computational Tools for Industrially Important Enzymes 383<br /> </b><i>Shweta Srivastava, Sakina Bombaywala, Hemant J. Purohit, and Nishant A. Dafale</i></p> <p>18.1 Introduction 383</p> <p>18.2 Strategies for Discovering Enzymes 385</p> <p>18.3 Computational Methods for Enzyme Function Prediction 391</p> <p>18.4 Conclusion 397</p> <p>Acknowledgments 398</p> <p>References 398</p> <p><b>19 Microbial Gallic Acid Decarboxylase: An Overview and Advancement in Application Potential Study Through Bioinformatics 407<br /> </b><i>Ishita Biswas, Debanjan Mitra, and Pradeep K. Das Mohapatra</i></p> <p>19.1 Introduction 407</p> <p>19.2 Isolation and Selection of Gallic Acid Decarboxylase Producing Microbes 407</p> <p>19.3 Purification of GAD Enzyme and Biomolecular Properties 409</p> <p>19.4 Gallic Acid Decarboxylase Assay 409</p> <p>19.5 Molecular Biological Aspects of Gallic Acid Decarboxylase with Special Reference to Human Gut Lactic Acid Bacteria 409</p> <p>19.6 In Silico Aspects of Gallic Acid Decarboxylase 411</p> <p>19.7 Discussion 416</p> <p>19.8 Conclusions 417</p> <p>References 417</p> <p><b>20 Microbial Chitinases: Potential Applications in Agriculture 421<br /> </b><i>Rupali Gupta, Gautam Anand, Dinesh Yadav, and Maya Bar</i></p> <p>20.1 Introduction 421</p> <p>20.2 Chitin Structure and Its Degradation by Chitinases 422</p> <p>20.3 Microbial Sources of Chitinase 424</p> <p>20.4 Chitinolytic Microorganisms as Potential Biological Control Agents or Biopesticides 425</p> <p>20.5 Metagenomic Approaches as a Tool to Unravel New Microbial Chitinases 428</p> <p>20.6 Conclusion and Future Perspectives 429</p> <p>References 431</p> <p><b>Volume 2</b></p> <p>About the Editors xvii</p> <p>Preface xxi</p> <p>21 β-Glucosidase Production and Its Applications 437<br /> <i>Vaibhav Badoni, Gaurav S. Rana, Ashutosh Dubey, and Ashok K. Verma</i></p> <p>22 Mining of Enzyme with Novel Activity Through Combination of Genomic Information andTraditional Biochemical Approach 477<br /> <i>Yutaka Kawarabayasi</i></p> <p>23 Strategies for Discovery and Enhancement of Enzyme Function: Current Developments andOpportunities 491<br /> <i>Menaka D. Salam, Swati Tripathi, and Simran</i></p> <p>24 Promises of Systems Biology to Better Understand the Kinetics of Industrially Important Enzymes505<br /> <i>Koel Mukherjee, Vinod Kumar Nigam, and Santhosh Pillai</i></p> <p>25 Metagenomics: New Insight in Microbial Diagnosis 519<br /> <i>Deepika Chaudhary, Aman Kumar, Kanisht Batra, and Sushila Maan</i></p> <p>26 Production and Therapeutic Applications of Monoclonal Antibodies in Cancer and Other Diseases535<br /> <i>Ambuj Shahi, Girijesh K. Patel, Sushil Kumar, Madhuri Singh, Anshika Varshney, and Uday C. Ghoshal</i></p> <p>27 Microbial Alkaline Protease: Production, Purification, and Applications 569<br /> <i>Degafneh Tadesse, Abate Ayele, Gamachis Korsa, and Chandran Masi</i></p> <p>28 Microbial Lipases in Modern Detergency: Sources, Production, and Application 593<br /> <i>Shashwat Katiyar and Nand Lal</i></p> <p>29 Production of Inulin Oligosaccharides from Microbial Inulinases and Their Applications 613<br /> <i>Priyanka, Hemant Ghai, and Wamik Azmi</i></p> <p>30 Characterization of Phytopathogen’s Tannase as a Virulence Factor 637<br /> <i>Richa Naredi and Kanti P. Sharma</i></p> <p>31 Mycozyme-based Functional Oligosaccharides 647<br /> <i>Suresh Nath, Hemant K. Rawat, Aamir Khan, and Naveen Kango</i></p> <p>32 Microbial Laccases: Structure, Function, and Applications 665<br /> <i>Supriya Gupta, Aiman Tanveer, Shruti Dwivedi, Vivek Kumar Morya, Manoj Kumar Yadav, and Dinesh Yadav</i></p> <p>33 Microbial ACC Deaminase: Stress Modulators in Plants 697<br /> <i>Lalita Pal, Vikas Dwivedi, Vikrant Dwivedi, and Diwakar M. Tripathi</i></p> <p>34 Ligninolytic Enzyme: Microbial Sources, Production, Purification, and Biotechnological Applications721<br /> <i>Rohida A. Hoque, Meera Yadav, and Hardeo S. Yadav</i></p> <p>35 Bioinformatic-driven Research in Microbial Enzymes: An Overview 739<br /> <i>Kanchan Yadav, Varsha Rani, Gautam Anand, Umesh Yadava, and Dinesh Yadav</i></p> <p>36 Food Waste and By-products: An Opportunity to Produce Enzymes for Industrial Applications 761<br /> <i>Manoj Tripathi, Ajay Yadav, Dilip Pawar, Rajpal S. Jadam, and RahulM.Srivastva</i></p> <p>37 Recombinant DNA Technology in the Improvement of Microbial Enzyme Production (Online)</p> <p>Index783</p>
<p><i><b>Dr. Dinesh Yadav, PhD,</b> Professor, Department of Biotechnology at Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, India. <p><b>Dr. Pankaj Chowdhary, PhD,</b> President, Society for Green Environment (SGE) at New Delhi, India. <p><b>Dr. Gautam Anand, PhD,</b> post-doctoral fellow at the Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), Volcani Institute, Rishon LeZion, Israel <p><b>Dr. Rajarshi Kumar Gaur, PhD,</b> Professor, Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India.</i>
<p><b>Comprehensive discussion of production and purification strategies for microbial enzymes important to various industries, from food and beverages to pharmaceuticals</b> <p><i>Microbial Enzymes</i> provides expert insight into diverse aspects of microbial enzymes, highlighting strategies for their production, purification, and manipulation, elucidating eco-friendly industrial applications, and discussing several production processes, such as the production of cellulose and non-synthetic indigo dye. This book emphasizes recent technological interventions in microbial enzyme technology like metagenomics, system biology, molecular biology, genomics, directed evolution, and bioinformatics. <p>The important microbial enzymes highlighted in this book include xylanases, ureases, methane monooxygenase, polyhydroxyalkanoates, pectinases, peroxidases, α-L-rhamnosidase, alkane hydroxylases, laccases, proteases, gallic acid decarboxylase, chitinases, beta-glucosidase, lipases, inulinases, tannase, mycozyme, ACC deaminase, ligninolytic enzymes, and many more. <p>Novel treatment methods involving strains of microorganisms with desirable properties applicable in the process of bioremediation through mitigating climate concern, increasing green production technology, improving agriculture productivity, and providing a means of earning a livelihood are discussed. Readers will also gain state-of-the-art background knowledge on existing technologies and their current challenges and future prospects. <p>Contributed to by leading experts in the field and edited by four highly qualified academics, <i>Microbial Enzymes</i> explores important topics including: <ul><li>Strategies for the discovery and enhancement of enzyme function, and potentials of system biology to better understand the kinetics of industrially important enzymes</li><li>Production and therapeutic applications of monoclonal antibodies in cancer and other diseases, and characterization of tannase as a virulence factor</li><li>Opportunities to produce enzymes through food waste and byproducts, and recent developments in computational tools</li><li>Use of Omics tools in the discovery of fungal enzymes and secondary metabolites</li></ul> <p><i>Microbial Enzymes</i> is a thorough and highly practical reference on the subject for students, scientists, biotechnologists, microbiologists, and policymakers working in environmental microbiology, biotechnology, and environmental sciences.

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