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<p>Contributors vii<br /> Preface xi</p> <p><b>Section I</b></p> <p><b>Diversity of CRISPR‐Cas Systems 1</b></p> <p>1 CRISPR-Cas Systems: Core Features and Common Mechanisms 3<br /> <i>Rodolphe Barrangou, Luciano A. Marraffini, and Erik J. Sontheimer</i></p> <p>2 Evolutionary Classification of CRISPR-Cas Systems 13<br /> <i>Kira S. Makarova, Yuri I. Wolf, and Eugene V. Koonin</i></p> <p>3 Molecular Mechanisms of Type I CRISPR-Cas Systems 39<br /> <i>John van der Oost</i></p> <p>4 Molecular Mechanisms of Type II CRISPR‐Cas Systems 53<br /> <i>Tautvydas Karvelis and Virginijus Siksnys</i></p> <p>5 Mechanism of Type III CRISPR‐Cas Immunity 71<br /> <i>Luciano A. Marraffini</i></p> <p>6 Type V CRISPR‐Cas Systems 85<br /> <i>Morgan Quinn Beckett, Anita Ramachandran, and Scott Bailey</i></p> <p>7 CRISPR-Cas13: Biology, Mechanism, and Applications of RNA-Guided, RNA-Targeting CRISPR Systems 99<br /> <i>Omar O. Abudayyeh and Jonathan S. Gootenberg </i></p> <p><b>Section II</b></p> <p><b>CRISPR‐Cas Biology 121</b></p> <p>8 CRISPR-Cas, Horizontal Gene Transfer, and the Flexible (Pan) Genome 123<br /> <i>Uri Gophna</i></p> <p>9 Evasion Tactics Manifested by Bacteriophages against Bacterial Immunity 131<br /> <i>Jenny Y. Zhang, Sutharsan Govindarajan, and Joseph Bondy-Denomy</i></p> <p>10 Regulation of CRISPR‐Cas Expression and Function 145<br /> <i>Leah M. Smith, Aroa Rey Campa, and Peter C. Fineran</i></p> <p><b>Section III</b></p> <p><b>CRISPR‐Based Technologies and Applications 163</b></p> <p>11 Genome Editing with CRISPR‐Cas Systems 165<br /> <i>Peter Lotfy and Patrick D. Hsu</i></p> <p>12 Genetic and Epigenetic Modulation of Gene Expression by CRISPR-dCas Systems 195<br /> <i>Jasprina N. Noordermeer, Crystal Chen, and Lei S. Qi</i></p> <p>13 CRISPR Screens 213<br /> <i>Jonathan D. D’Gama, Joseph S. Park, and Matthew K. Waldor</i></p> <p>14 CRISPR‐Based Antimicrobials 233<br /> <i>Justen Russell and David Bikard</i></p> <p>15 Exploiting CRISPR‐Cas Systems To Provide Phage Resistance in Industrial Bacteria 249<br /> <i>Rodolphe Barrangou and Avery Roberts</i></p> <p>16 Recording Biological Information with CRISPR‐Cas Systems 267<b><br /> </b><i>Mariia Y. Cherepkova, Tanmay Tanna, and Randall J. Platt</i></p> <p>Afterword 281</p> <p>Index 283</p>

<p><b> ABOUT THE EDITORS </b></p> <p><b>Rodolphe Barrangou</b> is the Todd R. Klaenhammer Distinguished Professor in the Department of Food, Bioprocessing and Nutrition Sciences at North Carolina State University; a member of the AAM, NAS, NAE, and NAI; a cofounder of Intellia Therapeutics, Locus Biosciences, Ancilia Biosciences, CRISPR Biotechnologies, and TreeCo; and Editor- in-Chief of <i>The CRISPR Journal.</i> <p><b>Erik J. Sontheimer</b> is the Pillar Chair in Biomedical Research and Professor in the RNA Therapeutics Institute at the University of Massachusetts Chan Medical School; an Associate Editor of <i>RNA</i>; a member of the AAM; a cofounder of Intellia Therapeutics; and a Scientific Advisory Board member at Tessera Therapeutics. <p><b>Luciano A. Marraffini</b> is the Kayden Family Professor and Head of the Laboratory of Bacteriology at The Rockefeller University; a Howard Hughes Medical Institute Investigator; a member of the AAM, AAAS, and NAS; and a cofounder of Intellia Therapeutics, CRISPR Biotechnologies, and Eligo Biosciences.

<p><b> CRISPR: BIOLOGY AND APPLICATIONS </b></p> <p><b>“<i>An excellent compendium of all things CRISPR from some of the leading minds in the field. With thorough coverage from every angle and beautifully detailed illustrations, this book is not to be missed!</i>” </b> <p><b>Jennifer A. Doudna, </b><BR> Professor of Chemistry, Biochemistry & Molecular Biology, UC Berkeley; Founder, Innovative Genomics Institute; Nobel Laureate and coinventor of CRISPR technology <p><b>“<i>This journey through CRISPR biology and several of its breakthrough applications offers an exciting glimpse into one of the most beautiful and compelling fields in the life sciences.</i>” </b> <p><b>David R. Liu,</b><BR>Director of the Merkin Institute at the Broad Institute of MIT and Harvard; Professor of Chemistry and Chemical Biology at Harvard University; coinventor of base editing and prime editing <p><b>“<i>A must read! The CRISPR topics, written by world-leading experts, span from the fascinating mechanistic underpinnings to the ingenious applications. One can read from start to finish or pick and choose themes. Either way, the book delivers utterly enjoyable learning!</i>” </b> <p><b> Bonnie Bassler, </b><BR>Squibb Professor and Chair, Princeton University Department of Molecular Biology; Howard Hughes Medical Institute Investigator <p>CRISPR-Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses. <p>Led by a distinguished team of editors, <i>CRISPR: Biology and Applications</i> explores the subject matter needed to delve into this fascinating area. Topics covered include: <ul><li>Classification and molecular mechanisms of CRISPR-Cas systems</li> <li>CRISPR-Cas evolution, regulation, expression, and function</li> <li>Uses for gene editing and modulation of gene expression </li> <li>CRISPR-based antimicrobials and phage resistance for medical and industrial purposes</li></ul> <p>Written by internationally renowned authors, <i>CRISPR: Biology and Applications</i> serves as both an introductory guide for those new to the field and an authoritative reference for seasoned researchers whose work touches this evolving and headline- making science.

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