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PERSPECTIVES IN PROTEOMICS SAMPLE PREPARATION<br> Introduction<br> General Aspects of Sample Preparation for Comprehensive Proteome Analysis<br> Proteomics: A Philosophical Perspective<br> MASS SPECTROMETRY<br> A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application<br> Sample Preparation for the Application of MALDI Mass Spectrometry<br> Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry<br> Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr<br> Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging<br> ELECTROPHORESIS<br> Sample Preparation for Two-Dimensional Gel Electrophoresis<br> Sample Preparation for Native Electrophoresis<br> Sample Preparation for LC-MS/MS using Free Flow Electrophoresis<br> Sample Preparation for Proteomics<br> OPTICAL METHODS<br> High-Throughout Proteomics: Spinning Disc Interferometry (SDI)<br> Optical Proteomics on Cell Arrays<br> Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis<br> Sample Preparation for Flow Cytometry<br> CHROMATOGRAPHY<br> Sample Preparation for HPLC-Based Proteome Analysis<br> Sample Preparation for Two-Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis<br> STRUCTURAL PROTEOMICS<br> Exploring Protein-Ligand Interactions by Solution NMR<br> Sample Preparation for Crystallography<br> INTERACTION ANALYSIS<br> Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP)<br> Methods<br> Exploring Membrane Proteomes<br> POST-TRANSLATIONAL MODIFICATIONS<br> Sample Preparation for Phosphoproteome Analysis<br> Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation<br> SPECIES-DEPENDENT PROTEOMICS<br> Sample Preparation and Data Processing in Plant Proteomics<br> Sample Preparation for MudPIT with Bacterial Protein Samples<br> Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi<br> THE HUMAN PROTEOSOME<br> Clinical Proteomics: Sample Preparation and Standardization<br> Stem Cell Proteomics<br> BIOINFORMATICS<br> Bioinformatics Support for Mass Spectrometric Quality Control<br> Use of Physio-Chemical Properties in Peptide and Protein Identification<br> <br> <br>

Jorg von Hagen is head of proteome research products development at Merck KGaA in Darmstadt (Germany). Having studied biotechnology in Giessen and Darmstadt, he received his academic degree with an award-winning thesis in 2001. Dr. von Hagen has more than 15 years of practical expertise in biotechnology, especially in molecular biology and proteomics methods for clinical applications.

This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.

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