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<p>Table of Contents</p> <p>0.1 Mapping the Essentials of Chemical Biology</p> <p>Chapter 1: The Structures of Biological Macromolecules and Lipid Assemblies</p> <p>1.1 General Introduction</p> <p>1.2 Protein Structures</p> <p>1.3 Carbohydrate Structures</p> <p>1.4 Nucleic Acid Structures</p> <p>1.5 Macromolecular Lipid Assemblies</p> <p>1.6 Structural Forces in Biological Macromolecules</p> <p>Chapter 2: Chemical and Biological Synthesis</p> <p>2.1 Introduction to Synthesis in Chemical Biology</p> <p>2.2 Chemical Synthesis of Peptides and Proteins</p> <p>2.3 Chemical Synthesis of Nucleic Acids</p> <p>2.4 Chemical Synthesis of Oligosaccharides</p> <p>2.5 Chemical Synthesis of Lipids</p> <p>2.6 Biological Synthesis of Biological Macromolecules</p> <p>2.7 Directed Biological Synthesis of Proteins</p> <p>2.8 Biological Syntheses of Nucleic Acids, Oligosaccharides and Lipids</p> <p>Chapter 3: Molecular Biology as a Toolset for Chemical Biology</p> <p>3.1 Key Concepts in Molecular Biology</p> <p>3.2 Tools and Techniques in Molecular Biology</p> <p>3.3 Cloning and Identification of Genes in DNA</p> <p>3.4 Integrating Cloning and Expression</p> <p>3.5 Site Directed Mutagenesis</p> <p>Chapter 4: Electronic and Vibrational Spectroscopy</p> <p>4.1 Electronic and Vibrational Spectroscopy in Chemical Biology</p> <p>4.2 UV-Visible Spectroscopy</p> <p>4.3 Circular Dichroism Spectroscopy</p> <p>4.4 Vibrational Spectroscopy</p> <p>4.5 Fluorescence Spectroscopy</p> <p>4.6 Probing Metal Centres in Biological Systems by Spectroscopy</p> <p>Chapter 5: Magnetic Resonance</p> <p>5.1 Magnetic Resonance in Chemical Biology</p> <p>5.2 Key Principles of NMR</p> <p>5.3 Two-dimensional NMR</p> <p>5.4 Multi-dimensional NMR</p> <p>5.5 Biological Macromolecule Structural Information</p> <p>5.6 EPR Spectroscopy; Key Principles</p> <p>Chapter 6: Diffraction and Microscopy</p> <p>6.1 Diffraction and Microscopy in Chemical Biology</p> <p>6.2 Key Principles of X-ray Diffraction</p> <p>6.3 Structural Information from X-ray Diffraction</p> <p>6.4 Neutron Diffraction</p> <p>6.5 Key Principles of Electron Microscopy</p> <p>6.6 Key Principles of Scanning Probe Microscopy</p> <p>Chapter 7: Molecular Recognition and Binding</p> <p>7.1 Molecular Recognition and Binding in Chemical Biology</p> <p>7.2 Theoretical Models of Binding</p> <p>7.3 Analysing Molecular Recognition and Binding</p> <p>7.4 Biological Molecular Recognition Studies</p> <p>Chapter 8: Kinetics and Catalysis</p> <p>8.1 Catalysis in Chemical Biology</p> <p>8.2 Steady State Kinetic Schemes</p> <p>8.3 Pre-steady State Kinetics</p> <p>8.4 Theories of Biocatalysis</p> <p>8.5 Electron Transfer</p> <p>Chapter 9: Mass Spectrometry and Proteomics</p> <p>9.1 Mass Spectrometry in Chemical Biology</p> <p>9.2 Key Principles in Mass Spectrometry</p> <p>9.3 Structural Analysis of Biological Macromolecules and Lipids by Mass Spectrometry</p> <p>9.4 The Challenge of Proteomics</p> <p>9.5 Genomics: Assigning Function to Genes and Proteins</p> <p>Chapter 10: Molecular Selection and Evolution</p> <p>10.1 Chemical Biology and the Origins of Life</p> <p>10.2 Molecular Breeding; Natural Selection Acting on Self-Organisation</p> <p>10.3 Directed Evolution of Protein Function</p> <p>10.4 Directed Evolution of Nucleic Acids</p> <p>10.5 Catalytic Antibodies</p> <p>Chapter 11: Chemical Biology of Cells</p> <p>11.1 General Introduction</p> <p>11.2 Array Technologies, Microfluidics, and Miniaturization</p> <p>11.3 Chemical Genetics and Potential New Therapeutics</p> <p>11.4 Chemical Cellular Dynamics</p> <p>11.5 Chemical Biology and in vivo Cell Connectomics</p> <p>Chapter 12: Chemical Biology of Stem Cells to Tissue Engineering</p> <p>12.1 General Introduction</p> <p>12.2 Chemical Stem Cell Biology</p> <p>12.3 The Road to Cell Therapies</p> <p>12.4 Tissue Engineering</p> <p>Chapter 13: Chemical Biology, Nanomedicine and Advanced Therapeutics</p> <p>13.1 General Introduction</p> <p>13.2 The Chemical Biology Approach to Gene Therapy</p> <p>13.3 Biophysical Characterisation of LNPs</p> <p>13.4 Applications of LNPs with Small Molecule Drugs</p> <p>Chapter 14: Chemical Biology and Advanced Diagnostics Leading to Precision</p> <p>Therapeutic Approaches</p> <p>14.1 General Introduction</p> <p>14.2 MRI Basic Principles Leading to Diagnostic Applications</p> <p>14.3 PET/CT and SPECT Fundamentals</p> <p>14.4 Understanding How to Control Nanoparticle Biodistribution Behaviour in vivo</p> <p>14.5 Theranostics</p> <p>Chapter 15: DNA Nanotechnology</p> <p>15.1 Background</p> <p>15.2 Three-Dimensional DNA Nanostructures</p> <p>15.3 Dynamic DNA Nanostructures</p> <p>15.4 Biomedical Applicationns of DNA Nanostructures</p>

<p><b>Andrew D. Miller, PhD,</b> is currently Professor of Organic Chemistry and Chemical Biology at Mendel University in Brno, Czech Republic, and founder/CSO of KP Therapeutics (Europe) s.r.o., a nanomedicine and precision therapeutics company, also based in the Czech Republic. <p><b>Julian A. Tanner, PhD,</b> is a Professor of the School of Biomedical Sciences, Assistant Dean of the LKS Faculty of Medicine, and Director of the Common Core at the University of Hong Kong.

<p><b>Discover a detailed knowledge of concepts and techniques that shape this unique multi-discipline</b> <p>Chemical Biology is devoted to understanding the way that Biology works at the molecular level. This is a problem-driven multi-discipline, incorporating as it does Organic, Physical, Inorganic, and Analytical Chemistry alongside newer emerging molecular disciplines. In recent years, Chemical Biology has emerged as a vibrant and growing multi-discipline distinct from Biochemistry that is focused on the quantitative analyses of the structures and functions of biological macromolecules and macromolecular lipid assemblies, at first in isolation, then <i>in vitro</i> and <i>in vivo</i>. <p>The second edition of the <i>Essentials of Chemical Biology</i> begins with a thorough introduction to the structure of biological macromolecules and macromolecular lipid assemblies, before moving on to the principles of chemical and biological synthesis, followed by descriptions of a comprehensive variety of research techniques and experimental methods. In addition, the second edition now includes new sections on the behaviour of biological macromolecules and macromolecular lipid assemblies in cells <i>in vitro</i> and in organisms <i>in vivo</i>. Given this, the second edition of the <i>Essentials of Chemical Biology</i> promises to cement itself as the leading introduction to Chemical Biology, incorporating descriptions of cutting-edge research wherever appropriate. Hence, readers of the second edition of the <i>Essentials of Chemical Biology</i> will find: <ul><li>a general expansion in understanding of basic molecular mechanisms in Biology moving towards cellular and organismal mechanisms</li> <li>entirely new chapters covering miniaturization and array technologies, Chemical Cell Biology, and the interface between Chemical Biology and Nanotechnology</li> <li>updates to chapters reflecting recent research developments</li> <li>an increased engagement with medical applications</li></ul> <p><i>Essentials of Chemical Biology</i> is ideal for advanced undergraduates or (post) graduate students in Chemical Biology and adjacent fields.

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