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<p><b>VOLUME I</b></p> <p>List of Contributors xi</p> <p>Preface xvii</p> <p>In Memoriam<br /> <i>Andrew Marston xix</i></p> <p><b>Part One: Sample Preparation and Identification 1</b></p> <p>1 Selection, Identification, and Collection of Plants 3<br /> <i>Monique S. J. Simmonds</i></p> <p>2 Extraction Methodologies: General Introduction 17<br /> <i>Valerie Camel</i></p> <p>3 Supercritical Fluid Extraction 43<br /> <i>Seied Mahdi Pourmortazavi, Mehdi Rahimi-Nasrabadi and Somayeh Mirsadeghi</i></p> <p>4 New Trends in Extraction of Natural Products: Microwave-Assisted Extraction and Pressurized Liquid Extraction 77<br /> <i>Philippe Christen and Beatrice Kaufmann</i></p> <p>5 Solid-Phase Microextraction (SPME) and Its Application to Natural Products 105<br /> <i>M. Abdul Mottaleb, Mohammed J. Meziani and M. Rafiq Islam</i></p> <p><b>Part Two: Instrumentation for Chemical Analysis 129</b></p> <p>6 Microscopic Analysis 131<br /> <i>Johannes Saukel and Elisabeth Ginko</i></p> <p>7 Thin-Layer Chromatography, with Chemical and Biological Detection Methods 185<br /> <i>Aurelie Urbain and Claudia Avello Simoes-Pires</i></p> <p>8 HPLC and Ultra HPLC: Basic Concepts 207<br /> <i>Veronika R. Meyer</i></p> <p>9 Near-Infrared (NIR) Spectroscopy in Natural Product Research 227<br /> <i>Christian W. Huck</i></p> <p>10 Headspace Sampling and Gas Chromatography: A Successful Combination to Study the Composition of a Plant Volatile Fraction 245<br /> <i>Barbara Sgorbini, Cecilia Cagliero, Chiara Cordero, Erica Liberto, Patrizia Rubiolo and Carlo Bicchi</i></p> <p>11 Analysis of Natural Products by Capillary Electrophoresis and Related Techniques 277<br /> <i>Markus Ganzera and Anja Kruger</i></p> <p>12 LC and LC-MS: Techniques and Applications 307<br /> <i>Nadja Arens, Stefanie Doell and Hans-Peter Mock</i></p> <p>13 NMR as Analytical Tool for Crude Plant Extracts 317<br /> <i>Anna R. Bilia</i></p> <p>14 NMR of Small Molecules 349<br /> <i>Christoph Seger</i></p> <p>15 NMR of Large Molecules 361<br /> <i>Christoph H. Wunderlich, Sarina Grutsch, Martin Tollinger and Christoph Kreutz</i></p> <p>16 On-Line and At-Line LC-NMR and Related Micro-NMR Methods 379<br /> <i>Nadine Bohni, Emerson F. Queiroz and Jean-Luc Wolfender</i></p> <p>17 New Developments of Laser Desorption Ionization Mass Spectrometry in Plant Analysis 411<br /> <i>Andreas Schinkovitz, Denis Seraphin and Pascal Richomme</i></p> <p><b>VOLUME II</b></p> <p>List of Contributors xi</p> <p>Preface xvii</p> <p>In Memoriam<br /> <i>Andrew Marston xix</i></p> <p><b>Part Three: Strategies for Selective Classes of Compounds 425</b></p> <p>18 Analysis of Plant Oligo- and Polysaccharides 427<br /> Wolfgang Blaschek</p> <p>19 Analytical Strategies for Multipurpose Studies of a Plant Volatile Fraction 447<br /> <i>Cecilia Cagliero, Barbara Sgorbini, Chiara Cordero, Erica Liberto, Carlo Bicchi and Patrizia Rubiolo</i></p> <p>20 Strategies for Lipid Analysis 467<br /> <i>Irina A. Guschina and John L. Harwood</i></p> <p>21 HPLC Analysis of Alkaloids 485<br /> <i>Bras Heleno de Oliveira</i></p> <p>22 Identification and Characterization of Hydroxycinnamates of Six <i>Galium </i>Species from the Rubiaceae Family 505<br /> <i>Rakesh Jaiswal, Marius F. Matei, Sagar Deshpande and Nikolai Kuhnert</i></p> <p>23 Identification and Characterization of Trimeric Proanthocyanidins of Two Members of the <i>Rhododendron </i>Genus (<i>Ericaceae</i>) by Liquid Chromatography Multi-Stage Mass Spectrometry 525<br /> <i>Rakesh Jaiswal, Mohamed G. E. Karar and Nikolai Kuhnert</i></p> <p>24 Strategies in the Analysis of Flavonoids 543<br /> <i>Celestino Santos-Buelga and Ana M. Gonzalez-Paramas</i></p> <p>25 Coumarins – Analytical and Preparative Techniques 569<br /> <i>Krystyna Skalicka-Wo´zniak and Kazimierz Głowniak</i></p> <p>26 Naphthoquinones and Anthraquinones: Chemical, Analytical, and Biological Overview 595<br /> <i>Nahed El-Najjar, Hala Gali-Muhtasib, Pia Vuorela, Arto Urtti and Heikki Vuorela</i></p> <p>27 Xanthones from Marine-Derived Microorganisms: Isolation, Structure Elucidation, and Biological Activities 611<br /> <i>Madalena M. M. Pinto, Raquel A. P. Castanheiro and Anake Kijjoa</i></p> <p>28 Sesquiterpenes and Other Terpenoids 633<br /> <i>Eirini Kouloura, Job Tchoumtchoua, Maria Halabalaki and Alexios-Leandros Skaltsounis</i></p> <p>29 Analysis of Plant Saponins 687<br /> <i>Justyna Krzyzanowska, Mariusz Kowalczyk and Wieslaw Oleszek</i></p> <p>30 Cardiotonic Glycosides 709<br /> <i>Liselotte Krenn</i></p> <p>31 Plant Steroids: Occurrence, Biological Significance, and Their Analysis 727<br /> <i>G. M. Kamal B. Gunaherath and A. A. Leslie Gunatilaka</i></p> <p>32 Chemical Analysis of Bryophytes 753<br /> <i>Yoshinori Asakawa, Agnieszka Ludwiczuk and Masao Toyota</i></p> <p>33 Cyclotide Analysis 807<br /> <i>Susan E. Northfield, Aaron G. Poth, Charlotte D’Souza and David J. Craik</i></p> <p><b>VOLUME III</b></p> <p>List of Contributors xi</p> <p>Preface xvii</p> <p>In Memoriam<br /> <i>Andrew Marston xix</i></p> <p><b>Part Four: Biological Analysis 825</b></p> <p>34 Phenotyping 827<br /> Qiaosheng Guo and Zaibiao Zhu</p> <p>35 Identification of Medicinal Plants Using DNA Barcoding 843<br /> <i>Xiaohui Pang and Shilin Chen</i></p> <p>36 Transcriptome Analysis of Medicinal Plants with Next-Generation Sequencing Technologies 847<br /> <i>Shilin Chen and Hongmei Luo</i></p> <p>37 Microarray 859<br /> <i>Chang Liu, Haimei Chen, Jianqin Li and Xiaolan Xu</i></p> <p>38 Small RNAs 875<br /> <i>Shanfa Lu</i></p> <p>39 Metabolomics 885<br /> <i>Jan Schripsema and Denise Dagnino</i></p> <p>40 Proteomics and Its Research Techniques in Plants 903<br /> <i>Liming Yang, Haibin Xu and Shilin Chen</i></p> <p>41 Multivariate Data Analysis 915<br /> <i>Bieke Dejaegher</i></p> <p><b>Part Five: Drugs from Plants 947</b></p> <p>42 <i>In Silico</i>-Guided Strategies for the Discovery and Rationalization of Bioactive Natural Products 949<br /> <i>Petra H. Pfisterer, Daniela Schuster, Judith M. Rollinger and Hermann Stuppner</i></p> <p>43 Innovative Strategies in the Search for Bioactive Plant Constituents 967<br /> <i>Emerson F. Queiroz and Jean-Luc Wolfender</i></p> <p>44 High Throughput Screening of Vegetal Natural Substances 987<br /> <i>Bruno David and Frederic Ausseil</i></p> <p>45 Mycotoxins Contamination in Food: Alternative Plant Preservatives, Legislation, and Detection Methods 1011<br /> <i>Monica R. Zuzarte, Ana P. Martins, Maria J. Goncalves and Lígia R. Salgueiro</i></p> <p>46 Quality Assessment of Herbal Drugs and Medicinal Plant Products 1039<br /> <i>Iqbal Ahmad, Mohd Sajjad Ahmad Khan and Swaranjit Singh Cameotra</i></p> <p><b>Part Six: Conclusion and Perspectives 1057</b></p> <p>47 Conclusion and Perspectives 1059</p> <p>Index 1061</p>

<p><b>Kurt Hostettmann</b> is the editor of <i>Handbook of Chemical and Biological Plant Analytical Methods</i>, 3 Volume Set, published by Wiley.</p> <p><b>Shilin Chen</b> is the editor of <i>Handbook of Chemical and Biological Plant Analytical Methods</i>, 3 Volume Set, published by Wiley.</p> <p><b>Andrew Marston</b> is the editor of <i>Handbook of Chemical and Biological Plant Analytical Methods</i>, 3 Volume Set, published by Wiley.</p> <p><b>Hermann Stuppner</b> is the editor of <i>Handbook of Chemical and Biological Plant Analytical Methods</i>, 3 Volume Set, published by Wiley.</p>

<p>Plants and plant-derived compounds and drugs are becoming more and more popular with increasing numbers of scientists researching plant analysis. The quality control of herbal drugs is also becoming essential to avoid severe health problems, and in the future many more new drugs will be developed from plant sources. This three-volume Handbook, featuring 47 detailed review articles, is unique as it deals with chemical and biological methodologies for plant analysis. Its aim is to present the most important and most accurate methods which are available for plant analysis. </p> <p>This comprehensive work is divided into six sections as follows: </p> <ul> <li><b>Sample preparation and identification</b> – discussing plant selection and collection, followed by extraction and sample preparation methodologies. </li> <li><b>Extraction and sample preparation methodologies</b></li> <li><b>Instrumentation for chemical analysis</b> - several instrumentations for chemical plant analysis are presented with an emphasis on hyphenated techniques, e.g. the coupling between HPLC and mass spectroscometry, and HPLC with NMR. </li> <li><b>Strategies for selective classes of compounds</b> – coverage of the most interesting classes of compounds such as polysaccharides, saponins, cardiotonic glycosides, alkaloids, terpenoids, lipids, volatile compounds and polyphenols ( flavonoids, xanthones, coumarins, naphthoquinones, anthraquinones, proanthocyanidins, etc.). </li> <li><b>Biological Analysis</b> - includes phenotyping, DNA barcoding techniques, transcriptome analysis , microarray, metabolomics and proteomics. </li> <li><b>Drugs from Plants</b> – covers the screening of plant extracts  and strategies for the quick discovery of novel bioactive natural products. Safety assessment of herbal drugs is highly dependent on outstanding chromatographic and spectroscopic methods which are also featured here.</li> </ul> <p>This Handbook aims to introduce to scientists involved in plant studies the current knowledge of methodologies in various fields of chemically- and biochemically-related topics in plant research.</p>

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