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<p>List of contributors, vii</p> <p>Preface, xi</p> <p>List of abbreviations, xiv</p> <p>1 An introduction to the third dimension for routine cell culture, 1<br /><i>Antonio Romo‐Morales and Stefan Przyborski</i></p> <p><b>Part I: Aggregate‐based technologies</b></p> <p>2 Gravity‐enforced microtissue engineering, 23<br /><i>Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm</i></p> <p>3 Physiologically relevant spheroid models for three‐dimensionalcell culture, 50<br /><i>Nicole A. Slawny and MaryAnn Labant</i></p> <p>4 NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture, 74<br /><i>Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman</i></p> <p>5 Micro‐moulded non‐adhesive hydrogels to form multicellular microtissues – the 3D Petri Dish^®, 97<br /><i>Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan</i></p> <p>6 Organotypic microtissues on an air‐liquid interface, 123<br /><i>Lars E. Sundstrom, Igor Charvet and Luc Stoppini</i></p> <p><b>Part II: Hydrogels</b></p> <p>7 Materials and assay systems used for three‐dimensional cell culture, 145<br /><i>Suparna Sanyal and Marshall Kosovsky</i></p> <p>8 HyStem^®, a customisable hyaluronan‐based hydrogel matrix for 3D cell culture, 173<br /><i>T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach‐Carson and D. D. Carson</i></p> <p>9 3‐D Life biomimetic hydrogels: A modular system for cell environment design, 197<br /><i>Brigitte M. Angres and Helmut Wurst</i></p> <p><b>Part III: Scaffolds</b></p> <p>10 Alvetex^®, a highly porous polystyrene scaffold for routine three‐dimensional cell culture, 225<br /><i>Antonio Romo‐Morales, Eleanor Knight and Stefan Przyborski</i></p> <p>11 CelluSponge™ and Go Matrix as innovative three‐dimensional cell culture platforms, 250<br /><i>Bramasta Nugraha</i></p> <p>12 Mimetix^® electrospun scaffold: An easy‐to‐use tool for 3D cell culture in drug discovery and regenerative medicine, 284<br /><i>Robert J. McKean and Elena Heister</i></p> <p><b>Part IV: 3D bioreactor technologies</b></p> <p>13 Quasi Vivo^® bioreactor technology, 305<br /><i>J. Malcolm Wilkinson</i></p> <p>14 Three‐dimensional cell‐based assays in hollow fibre bioreactors, 327<br /><i>John J. S. Cadwell and William G. Whitford</i></p> <p>15 Three‐dimensional engineered tissues for high‐throughput compound screening: Mechanical properties of skin and ageing, 351<br /><i>Michael Conway, Ayla Annac and Tetsuro Wakatsuki</i></p> <p>16 Three‐dimensional cell culture in the Rotary Cell Culture System™, 370<br /><i>Stephen S. Navran</i></p> <p>Glossary, 386</p> <p>Index, 393</p>

<p><b>Stefan Przyborski </b>is Professor of Cell Technology in the Department of Biosciences, Durham University, UK. He is also the Chief Scientific Officer for ReproCELL Europe, UK. A recognised leader in the field of 3D cell culture, Professor Przyborski has published extensively in this field and presents his work regularly at international events.</p>

<p>With the recognition that cells live in complex, three-dimensional (3D) tissue, it should come as no surprise that many laboratories are transitioning from conventional two-dimensional (2D) cell culture in Petri dishes to 3D cell culture techniques – but where to begin? <i>Technology Platforms for 3D Cell Culture: A User’s Guide </i>points to the current options available to perform 3D culture, shows where such technology is available, explains how it works and reveals how it can be used by scientists working in their own labs.</p> <p>Featuring contributions from leading developers and researchers at the cutting edge of today’s 3D cell technology, the chapters present a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture, along with clear instructions and guidance on performing specific 3D culture methods. Colour illustrations are included, along with examples of where such technologies have been successfully applied, information on resources and technical support to help initiate the use of 3D culture methods.</p> <p><i>Technology Platforms for 3D Cell Culture: A User’s Guide </i>is an indispensable single-volume resource for students, laboratory-based scientists and cell biologists seeking knowledge of the state-of-the-art technologies currently available for 3D cell culture.</p> <p>www.wiley.com/wiley-blackwell</p>

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