Cell Culture Engineering, by GyunMin Lee

Luisi, P.P. (ed.)

Chemical Synthetic Biology


Print ISBN: 978‐0‐470‐71397‐6

Further Volumes of the “Advanced Biotechnology” Series:


Villadsen, J. (ed.)

Fundamental Bioengineering


Print ISBN: 978‐3‐527‐33674‐6

Love, J. Ch. (ed.)

Micro‐ and Nanosystems for Biotechnology


Print ISBN: 978‐3‐527‐33281‐6

Wittmann, Ch., Liao, J.C. (eds.)

Industrial Biotechnology

Microorganisms (2 Volumes)


Print ISBN: 978‐3‐527‐34179‐5

Wittmann, Ch., Liao, J.C. (eds.)

Industrial Biotechnology

Products and Processes


Print ISBN: 978‐3‐527‐34181‐8

Yoshida, T. (ed.)

Applied Bioengineering


Print ISBN: 978‐3‐527‐34075‐0

Nielsen, J., Hohmann, S. (eds.)

Systems Biology


Print ISBN: 978‐3‐527‐33558‐9

Smolke, C.

Synthetic Biology

Parts, Devices, and Applications


Print ISBN: 978‐3‐527‐33075‐1

Chang, H. N.

Emerging Areas in Bioengineering


Print ISBN: 978‐3‐527‐34088‐0


Zhao, H.

Protein Engineering

Tools and Applications


Print ISBN: 978‐3‐527‐34470‐3

Cell Culture Engineering

Recombinant Protein Production

Edited by

Gyun Min Lee

Helene Faustrup Kildegaard

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About the Series Editors


Sang Yup Lee is a distinguished Professor at the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology. At present, Prof. Lee is the Director of the Center for Systems and Synthetic Biotechnology, Director of the BioProcess Engineering Research Center, and Director of the Bioinformatics Research Center. He has published more than 500 journal papers, 64 books, and book chapters and has more than 580 patents (either registered or applied) to his credit. He has received numerous awards, including the National Order of Merit, the Merck Metabolic Engineering Award, the ACS Marvin Johnson Award, Charles Thom Award, Amgen Biochemical Engineering Award, Elmer Gaden Award, POSCO TJ Park Prize, and HoAm Prize. He is a fellow of American Association for the Advancement of Science, the American Academy of Microbiology, American Institute of Chemical Engineers, Society for Industrial Microbiology and Biotechnology, American Institute of Medical and Biological Engineering, the World Academy of Science, the Korean Academy of Science and Technology, and the National Academy of Engineering of Korea. He is also a foreign member of National Academy of Engineering, USA. In addition, he is an honorary professor of the University of Queensland (Australia), the Chinese Academy of Sciences, Wuhan University (China), Hubei University of Technology (China), and Beijing University of Chemical Technology (China) and an advisory professor of the Shanghai Jiaotong University (China). Apart from his academic associations, Prof. Lee is the editor‐in‐chief of the Biotechnology Journal and is also contributing to numerous other journals as associate editor and board member. Prof. Lee is serving as a member of Presidential Advisory Committee on Science and Technology (South Korea).


Jens Nielsen is a professor and director of Chalmers University of Technology (Sweden) since 2008. He obtained an MSc degree in Chemical Engineering and a PhD degree (1989) in Biochemical Engineering from the Technical University of Denmark (DTU), and after that, established his independent research group and was appointed a full‐time professor there in 1998. He was a Fulbright visiting professor at MIT in 1995–1996. At DTU, he founded and directed the Center for Microbial Biotechnology. Prof. Nielsen has published more than 350 research papers and coauthored more than 40 books, and he is the inventor of more than 50 patents. He has founded several companies that have raised more than 20 million in venture capital. He has received numerous Danish and international awards and is a member of the Academy of Technical Sciences (Denmark), the National Academy of Engineering (USA), the Royal Danish Academy of Science and Letters, the American Institute for Medical and Biological Engineering, and the Royal Swedish Academy of Engineering Sciences.


Gregory Stephanopoulos is the W.H. Dow Professor of Chemical Engineering at the Massachusetts Institute of Technology (MIT, USA) and Director of the MIT Metabolic Engineering Laboratory. He is also an instructor of bioengineering at Harvard Medical School (since 1997). He received his BS degree from the National Technical University of Athens and PhD from the University of Minnesota (USA). He has coauthored about 400 research papers and 50 patents, along with the first textbook on metabolic engineering. He has been recognized by numerous awards from the American Institute of Chemical Engineers (AIChE) (Wilhelm, Walker and Founders awards), American Chemical Society (ACS), Society of Industrial Microbiology (SIM), BIO (Washington Carver Award), the John Fritz Medal of the American Association of Engineering Societies, and others. In 2003, he was elected as a member of the National Academy of Engineering (USA) and in 2014 president of AIChE.