Table of Contents
Title Page
Copyright
Foreword
Preface
Part I: Hydrodynamic Flow Confinement (HFC)
Chapter 1: Hydrodynamic Flow Confinement Using a Microfluidic Probe
1.1 Introduction
1.2 HFC Principle
1.3 MFP Heads
1.4 Vertical MFP
1.5 Advanced MFP Heads and Holders
1.6 Surface Processing Using an MFP
1.7 MFP Components
1.8 Outlook
Acknowledgments
References
Chapter 2: Hierarchical Hydrodynamic Flow Confinement (hHFC) and Recirculation for Performing Microscale Chemistry on Surfaces
2.1 Introduction
2.2 Hierarchical HFC
2.3 Recirculation
2.4 Microscale Deposition
Acknowledgments
References
Chapter 3: Design of Hydrodynamically Confined Microflow Devices with Numerical Modeling: Controlling Flow Envelope, Pressure, and Shear Stress
3.1 Introduction
3.2 Theory
3.3 Device and Experimental Methods for CFD Validation
3.4 Numerical Modeling of HCM devices
3.5 Envelope Size and Pressure Drop Across HCMs
3.6 Hydrodynamic Loads Generated by HCM Devices
3.7 Concluding Remarks
References
Chapter 4: Hele-Shaw Flow Theory in the Context of Open Microfluidics: From Dipoles to Quadrupoles
4.1 Introduction
4.2 Fundamentals of Hele-Shaw Flows
4.3 Applications to Microfluidic Dipoles and Quadrupoles
4.4 Diffusion in Hele-Shaw Flows
4.5 Conclusion
References
Chapter 5: Implementation and Applications of Microfluidic Quadrupoles
5.1 Introduction
5.2 Principles and Configurations of MQs
5.3 Implementation of MQs
5.4 MQ Analysis and Characterization
5.5 Application of MQs in Biology and Life Sciences
5.6 Summary and Outlook
References
Chapter 6: Hydrodynamic Flow Confinement-Assisted Immunohistochemistry from Micrometer to Millimeter Scale
6.1 Immunohistochemical Analysis of Tissue Sections
6.2 Probe Heads for Multiscale Surface Interactions
6.3 Immunohistochemistry with Microfluidic Probes
6.4 Micro-IHC on Human Tissue Sections
6.5 Millimeter-Scale Immunohistochemistry
6.6 Outlook
Acknowledgments
References
Chapter 7: Local Nucleic Acid Analysis of Adherent Cells
7.1 Introduction
7.2 Methods
7.3 Results
7.4 Discussion
7.5 Concluding Remarks
Acknowledgments
References
Chapter 8: Microfluidic Probe for Neural Organotypic Brain Tissue and Cell Perfusion
8.1 Introduction
8.2 Microperfusion of Organotypic Brain Slices Using the Microfluidic Probe
8.3 Microperfusion of Live Dissociated Neural Cell Cultures Using the Microfluidic Probe
8.4 Conclusion
Acknowledgments
References
Chapter 9: The Multifunctional Pipette
9.1 Introduction
9.2 Open Volume Probes
9.3 Detailed View on the Multifunctional Pipette
9.4 Integrated Functions
9.5 Functional Extensions and Applications
9.6 Future Technology
Acknowledgments
References
Chapter 10: Single-Cell Analysis with the BioPen
10.1 Introduction
10.2 The Single-Cell Challenge
10.3 Superfusion Techniques
10.4 The BioPen
10.5 Application Areas
10.6 Future Technology
Acknowledgments
References
Chapter 11: Microfluidic Probes for Single-Cell Proteomic Analysis
11.1 Introduction
11.2 Technical Requirements of Single-Cell Proteomic Analysis
11.3 Methods for Single-Cell Proteomic Analysis
11.4 Microfluidics Enabling Next-Generation Single-Cell Proteomics
11.5 Open-Ended Microwells for Proteomic and Multiparameter Single-Cell Studies
11.6 Microfluidic Probes in
In Situ
Single-Cell Proteomic Measurement
11.7 Outlook for Future Work with Microfluidic Single-Cell Proteomic Assay
11.8 Conclusion
References
Part II: Localized Chemistry
Chapter 12: Aqueous Two-Phase Systems for Micropatterning of Cells and Biomolecules
12.1 Introduction
12.2 Small Molecules Applications
12.3 Cell Patterning
12.4 Conclusions
Acknowledgments
References
Chapter 13: Development of Pipettes as Mobile Nanofluidic Devices for Mass Spectrometric Analysis
13.1 Introduction
13.2 Segmented Flow Analysis
13.3 Utility of Nano- and Micropipettes in Mass Spectrometry
13.4 Development of Nanopipette Probes for Local Sampling
13.5 MALDI-MS Analysis of Analyte Post-Nanopipette Sampling
13.6 Development of Segmented Flow Sampling
13.7 Study of Intercellular Heterogeneity
13.8 Conclusion and Outlook
Acknowledgments
References
Chapter 14: FluidFM: Development of the Instrument as well as Its Applications for 2D and 3D Lithography
14.1 Microchanneled AFM Cantilevers
14.2 Development of the FluidFM
14.3 Calibration of Hollow Probes: Stiffness and Flow
14.4 FluidFM as Lithography Tool in Liquid
14.5 Conclusions and Outlook
Acknowledgments
References
Chapter 15: FluidFM Applications in Single-Cell Biology
15.1 Introduction
15.2 Nondestructive Cell Manipulations
15.3 Spatial Cell Manipulation
15.4 Controlled Fluid Delivery
15.5 Mechanical Measurements
15.6 Ionic Current Measurements
15.7 Molecular Analyses
15.8 Conclusion and Future Perspectives
References
Chapter 16: Soft Probes for Scanning Electrochemical Microscopy
16.1 Introduction
16.2 Principles of Scanning Electrochemical Microscopy (SECM)
16.3 Soft Probes for SECM
16.4 Applications of Soft SECM Probes
16.5 Conclusions and Future Perspectives
References
Chapter 17: Microfluidic Probes for Scanning Electrochemical Microscopy
17.1 Introduction
17.2 Combining Microfluidics with SECM
17.3 Electrochemical Characterization
17.4 Applications
17.5 Conclusions and Outlook
References
Chapter 18: Chemistrode for High Temporal- and Spatial-Resolution Chemical Analysis
18.1 Introduction
18.2 Chemistrode Design and Operation
18.3 Physical Principles Governing the Transport Processes
18.4 Multiform Chemical Analysis Independent in Space and Time from Data Acquisition
18.5 Applicability for Stimuli–Response Surfaces
18.6 Challenges and Future Directions
Acknowledgments
References
Index
End User License Agreement
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Guide
Cover
Table of Contents
Foreword
Preface
Part I: Hydrodynamic Flow Confinement (HFC)
Begin Reading