Preface<br> Introduction: Application of proteomic technologies for the analysis of microbial infections<br> (Jiri Stulik and Patrick Butaye)<br> <br> BASIC PROTEOMIC METHODS<br> Separation of proteins and peptides<br> (Ludovit Skultety)<br> Basic mass spectrometric approaches<br> (Lenka Hernychova and Martin Hubalek)<br> Quantitative mass spectrometric approaches<br> (Juraj Lenco and Vojtech Tambor)<br> BN-PAGE of microbial protein complexes<br> (Jiri Dresler and Jana Klimentova)<br> Analysis of Francisella tularensis glycoproteins<br> (Lenka Hernychova and Lucie Balonova)<br> <br> IDENTIFICATION OF PROTEINS AS WELL AS GLYCANS FROM MICROORGANISMS AS CANDIDATE MOLECULES FOR USE IN DETECTION/DIAGNOSIS, THERAPY AND PROPHYLAXIS<br> Comparative proteome analysis of strains with differential virulence<br> (Martin Hubalek and Ivona Pavkova)<br> Analysis of Francisella tularensis acetonitrile extracts<br> (Lenka Hernychova, Martin Hubalek and J. Udrzalova)<br> Analysis of culture filtrate proteins of Francisella tularensis<br> (Klara Konecna and Lenka Hernychova)<br> Lipopolysaccharides of Coxiella burnetii: Chemical composition and structure, and their role in diagnosis of Q Fever<br> (Rudolf Toman and Pavol Vadovic)<br> Mimivirus possess anonymous and unique gene products<br> endowed for antigenic properties<br> (Patricia Renesto and Didier Raoult)<br> Detection of differentially modified pathogen proteins by Western Blot after<br> 2-D gel electrophoresis and identification by MALDI-TOF/TOF<br> (Fred Fack, Julia Kessler, Patrick Pirrotte, Jacques Kremer, Dominique Revets, Wim Ammerlaan and Claude P. Muller)<br> Composition and structure of lipids A of the intracellular bacteria Piscirickettsia salmonis and Coxiella burnetii<br> (Pavol Vadovic, Robert Ihnatko and Rudolf Toman)<br> Proteins of Coxiella burnetii and analysis of their function<br> (Robert Ihnatko, Pavol Vadovic and Rudolf Toman)<br> Subtype and toxin variant identification of Botulinum neurotoxin Type A using proteomics techniques<br> (Suzanne R. Kalb, Jakub Baudys, Theresa J. Smith, James L. Pirkle and John R. Barr)<br> Protein microarrays for antigen discovery<br> (Mohan Natesan, Sarah Keasey and Robert G. Ulrich)<br> MALDI-TOF mass spectrometry for rapid identification of highly pathogenic microorganisms<br> (Peter Lasch and Dieter Naumann)<br> <br> ANALYSIS OF HOST PATHOGEN INTERACTIONS<br> Quantitative proteomic profiling of host - pathogen interactions: The interaction of Francisella tularensis LVS with macrophages using J774.2 cell line<br> (Anetta Hartlova, Marek Link, Juraj Lenco and Jiri Stulik)<br> Proteome analysis of bacterial protein expression after ingestion of microbes by macrophages<br> (Martin Brychta and Ivona Pavkova)