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<p><b>Comprehensive coverage of the entire induced pluripotent stem cell basic work flow</b> </p> <p>Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.</p> <p><i>Human Pluripotent Stem Cells: A Practical Guide</i> offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.</p> <ul> <li>Includes helpful tips and tricks that are normally omitted from regular research papers</li> <li>Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations</li> <li>Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner</li> <li>Written by experts with extensive experience in stem cell technologies</li> </ul> <p><i>Human Pluripotent Stem Cells: A Practical Guide</i> is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.</p>