Details

Manual of Molecular and Clinical Laboratory Immunology


Manual of Molecular and Clinical Laboratory Immunology


ASM Books 8. Aufl.

von: Barbara Detrick, John L. Schmitz, Robert G. Hamilton

188,99 €

Verlag: ASM Press
Format: EPUB
Veröffentl.: 02.05.2016
ISBN/EAN: 9781683673262
Sprache: englisch

DRM-geschütztes eBook, Sie benötigen z.B. Adobe Digital Editions und eine Adobe ID zum Lesen.

Beschreibungen

<p><b>THE authoritative guide for clinical laboratory immunology</b></p> <p>For over 40 years the <i>Manual of Molecular and Clinical Laboratory Immunology</i> has served as the premier guide for the clinical immunology laboratory. From basic serology testing to the present wide range of molecular analyses, the <i>Manual</i> has reflected the exponential growth in the field of immunology over the past decades. This eighth edition reflects the latest advances and developments in the diagnosis and treatment of patients with infectious and immune-mediated disorders.</p> The <i>Manual</i> features detailed descriptions of general and specific methodologies, placing special focus on the interpretation of laboratory findings, and covers the immunology of infectious diseases, including specific pathogens, as well as the full range of autoimmune and immunodeficiency diseases, cancer, and transplantation. <p>Written to guide the laboratory director, the <i>Manual</i> will also appeal to other laboratory scientists, especially those working in clinical immunology laboratories, and pathologists. It is also a useful reference for physicians, mid-level providers, medical students, and allied health students with an interest in the role that immunology plays in the clinical laboratory.</p>
<p>Editorial Board xi</p> <p>Contributors xiii</p> <p>Foreword: How it Began xxiii</p> <p>Preface xxv</p> <p>Author and Editor Conflicts of Interest xxvii</p> <p><b>Section A General Methods 1</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>Thomas A. Fleisher</i></p> <p>1 Introduction 3<br /><i>Thomas A. Fleisher</i></p> <p>2 Molecular Methods for Diagnosis of Genetic Diseases Involving the Immune System 5<br /><i>Amy P. Hsu</i></p> <p>3 The Human Microbiome and Clinical Immunology 19<br /><i>Frederic D. Bushman</i></p> <p>4 Protein Analysis in the Clinical Immunology Laboratory 26<br /><i>Roshini Sarah Abraham and David R. Barnidge</i></p> <p><b>Section B Immunoglobulin Methods =/ 47</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>David F. Keren</i></p> <p>5 Introduction 49<br /><i>David F. Keren</i></p> <p>6 Immunoglobulin Genes 51<br /><i>Thomas J. Kipps, Emanuela M. Ghia, and Laura Z. Rassenti</i></p> <p>7 Immunoglobulin Quantification and Viscosity Measurement 65<br /><i>Jeffrey S. Warren</i></p> <p>8 Clinical Indications and Applications of Serum and Urine Protein Electrophoresis 74<br /><i>David F. Keren and Richard L. Humphrey</i></p> <p>9 Immunochemical Characterization of Immunoglobulins in Serum, Urine, and Cerebrospinal Fluid 89<br /><i>Elizabeth Sykes and Yvonne Posey</i></p> <p>10 Cryoglobulins, Cryofibrinogenemia, and Pyroglobulins 101<br /><i>Peter D. Gorevic and Dennis Galanakis</i></p> <p>11 Strategy for Detecting and Following Monoclonal Gammopathies 112<br /><i>Jerry A. Katzmann and David F. Keren</i></p> <p><b>Section C Complement 125</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>Patricia C. Giclas</i></p> <p>12 Introduction 127<br /><i>Patricia C. Giclas</i></p> <p>13 The Classical Pathway of Complement 129<br /><i>Patricia C. Giclas</i></p> <p>14 Analysis of Activity of Mannan-Binding Lectin, an Initiator of the Lectin Pathway of the Complement System 133<br /><i>Steffen Thiel</i></p> <p>15 The Nature of the Diseases That Arise from Improper Regulation of the Alternative Pathway of Complement 138<br /><i>Richard J. H. Smith</i></p> <p><b>Section D Flow Cytometry 145</b></p> <p>Volume Editor: <i>John L. Schmitz</i></p> <p>Section Editor: <i>Maurice R. G. O’gorman</i></p> <p>16 Introduction 147<br /><i>Maurice R. G. O’gorman</i></p> <p>17 Polychromatic Flow Cytometry 149<br /><i>Angélique Biancotto and </i><i>J. Philip Mccoy, Jr.</i></p> <p>18 High-Sensitivity Detection of Red and White Blood Cells in Paroxysmal Nocturnal Hemoglobinuria by Multiparameter Flow Cytometry 168<br /><i>Andrea Illingworth, Michael Keeney, and D. Robert Sutherland</i></p> <p>19 Standardized Flow Cytometry Assays for Enumerating CD34+ Hematopoietic Stem Cells 182<br /><i>D. Robert Sutherland and Michael Keeney</i></p> <p>20 Functional Flow Cytometry-Based Assays of Myeloid and Lymphoid Functions for the Diagnostic Screening of Primary Immunodeficiency Diseases 199<br /><i>Maurice R. G. O’gorman</i></p> <p>21 Acute Lymphoblastic Leukemia/Lymphoma: Diagnosis and Minimal Residual Disease Detection by Flow Cytometric Immunophenotyping 207<br /><i>Joseph A. Digiuseppe</i></p> <p>22 Acute Myeloid Leukemia: Diagnosis and Minimal Residual Disease Detection by Flow Cytometry 217<br /><i>Brent Wood and Lori Soma</i></p> <p>23 Chronic Lymphocytic Leukemia, the Prototypic Chronic Leukemia for Flow Cytometric Analysis 226<br /><i>Heba Degheidy, Dalia A. A. Salem, Constance M. Yuan, and Maryalice Stetler-Stevenson</i></p> <p>24 Plasma Cell Disorders 235<br /><i>Juan Flores-Montero, Luzalba Sanoja, José Juan Pérez, Fanny Pojero, Noemí Puig, María</i> <i>Belén Vidriales, and Alberto Orfao</i></p> <p>25 Future Cytometric Technologies and Applications 251<br /><i>Holden T. Maecker</i></p> <p><b>Section E Functional Cellular Assays 259</b></p> <p>Volume Editor: <i>Barbara Detrick</i></p> <p>Section Editor: <i>Steven D. Douglas</i></p> <p>26 Introduction 261<br /><i>Steven D. Douglas</i></p> <p>27 Cryopreservation of Peripheral Blood Mononuclear Cells 263<br /><i>Adriana Weinberg</i></p> <p>28 Lymphocyte Activation 269<br /><i>Roshini Sarah Abraham</i></p> <p>29 Functional Assays for B Cells and Antibodies 280<br /><i>Moon H. Nahm and Robert L. Burton</i></p> <p>30 Methods for Detection of Antigen-Specific T Cells by Enzyme-Linked Immunospot Assay (ELISPOT) 290<br /><i>Barbara L. Shacklett and Douglas F. Nixon</i></p> <p>31 Regulatory T Cell (Treg) Assays: Repertoire, Functions, and Clinical Importance of Human Treg 296<br /><i>Theresa L. Whiteside</i></p> <p>32 Measurement of NK Cell Phenotype and Activity in Humans 300<br /><i>Samuel C. C. Chiang and Yenan T. Bryceson</i></p> <p>33 Functional Assays for the Diagnosis of Chronic Granulomatous Disease 310<br /><i>Debra Long Priel and Douglas B. Kuhns</i></p> <p><b>Section F Cytokines and Chemokines 321</b></p> <p>Volume Editor: <i>Barbara Detrick</i></p> <p>Section Editor: <i>John J. Hooks</i></p> <p>34 Introduction 323<br /><i>John J. Hooks</i></p> <p>35 Multiplex Cytokine Assays 324<br /><i>Elizabeth R. Duffy and Daniel G. Remick</i></p> <p>36 Cytokine Measurement by Flow Cytometry 338<br /><i>Holden T. Maecker</i></p> <p>37 Chemokine and Chemokine Receptor Analysis 343<br /><i>Sabina A. Islam, Benjamin D. Medoff, and Andrew D. Luster</i></p> <p>38 Cytokines: Diagnostic and Clinical Applications 357<br /><i>Priyanka Vashisht and Timothy B. Niewold</i></p> <p>39 Detection of Anticytokine Autoantibodies and Clinical Applications 365<br /><i>S Arah K. Browne</i></p> <p><b>Section G Immunohistology and Immunopathology 373</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>R. Neal Smith</i></p> <p>40 Introduction 375<br /><i>Robert G. Hamilton</i></p> <p>41 Immunofluorescence Methods in the Diagnosis of Renal and Cardiac Diseases 376<br /><i>A. Bernard Collins, James R. Stone, and R. Neal Smith</i></p> <p>42 Western Blot Analysis for the Detection of Anti-Glomerular Basement Membrane Antibodies and Anti-Phospholipase A2 Receptor Antibodies 385<br /><i>A. Bernard Collins and R. Neal Smith</i></p> <p><b>Section H Infectious Diseases Caused by Bacteria, Mycoplasmas, Chlamydiae, and Rickettsiae 391</b></p> <p>Volume Editor: <i>John L. Schmitz</i></p> <p>Section Editor: <i>Christine M. Litwin</i></p> <p>43 Introduction 393<br /><i>Christine M. Litwin</i></p> <p>44 Diagnostic Methods for Group A Streptococcal Infections 394<br /><i>Christine M. Litwin, Sheldon E. Litwin, and Harry R. Hill</i></p> <p>45 Diagnosis of Helicobacter pylori Infection andAssessment of Eradication 404<br /><i>Bruce E. Dunn and Suhas H. Phadnis</i></p> <p>46 Laboratory Diagnosis of Syphilis 412<br /><i>John L. Schmitz</i></p> <p>47 Lyme Disease, Relapsing Fever, and Leptospirosis 419<br /><i>Guiqing Wang and Maria E. Aguero-Rosenfeld</i></p> <p>48 Immunological Tests in Tuberculosis 433<br /><i>Christine M. Litwin</i></p> <p>49 Mycoplasma: Immunologic and Molecular Diagnostic Methods 444<br /><i>Ken B. Waites, Mary B. Brown, and Jerry W. Simecka</i></p> <p>50 Chlamydia and Chlamydophila Infections 453<br /><i>Rosemary She</i></p> <p>51 The Rickettsiaceae, Anaplasmataceae, and Coxiellaceae 461<br /><i>Lucas S. Blanton and David H. Walker</i></p> <p>52 The Bartonellaceae, Brucellaceae, and Francisellaceae 473<br /><i>Christine M. Litwin, Burt Anderson, Renee Tsolis, and Amy Rasley</i></p> <p><b>Section I Mycotic and Parasitic Diseases 483</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>Thomas B. Nutman</i></p> <p>53 Introduction 485<br /><i>Thomas B. Nutman</i></p> <p>54 Immunological and Molecular Approaches for the Diagnosis of Parasitic Infections 486<br /><i>Patricia P. Wilkins and Thomas B. Nutman</i></p> <p>55 Serological and Molecular Diagnosis of Fungal Infections 503<br /><i>Mark D. Lindsley</i></p> <p><b>Section J Viral Diseases 535</b></p> <p>Volume Editor: <i>John L. Schmitz</i></p> <p>Section Editors: <i>Richard L. Hodinka and John L. Schmitz</i></p> <p>56 Introduction 537<br /><i>John L. Schmitz</i></p> <p>57 Immunologic and Molecular Methods for Viral Diagnosis 538<br /><i>Marie Louise Landry and Yi-Wei Tang</i></p> <p>58 Herpes Simplex Virus 550<br /><i>D. Scott Schmid</i></p> <p>59 Varicella-Zoster Virus 556<br /><i>D. Scott Schmid</i></p> <p>60 Epstein-Barr Virus and Cytomegalovirus 563<br /><i>Henry H. Balfour, Jr., Kristin A. Hogquist, and Priya S. Verghese</i></p> <p>61 Human Herpesviruses 6, 7, and 8 578<br /><i>Richard L. Hodinka</i></p> <p>62 Parvovirus B19 591<br /><i>Stanley J. Naides</i></p> <p>63 Respiratory Viruses 598<br /><i>David J. Speicher, Mohsin Ali, and Marek Smieja</i></p> <p>64 Measles, Mumps, and Rubella Viruses 610<br /><i>Diane S. Leland and Ryan F. Relich</i></p> <p>65 Viral Hepatitis 620<br /><i>Hubert G. M. Niesters, Annelies Riezebos-Brilman, and Coretta C. Van Leer-Buter</i></p> <p>66 Viral Agents of Gastroenteritis 639<br /><i>Gabriel I. Parra and Kim Y. Green</i></p> <p>67 Arboviruses 648<br /><i>Robert S. Lanciotti and John T. Roehrig</i></p> <p>68 Diagnosis of Hantavirus Infections 658<br /><i>William Marciel De Souza and Luiz Tadeu Moraes Figuereido</i></p> <p>69 Rabies Virus 665<br /><i>D. Craig Hooper</i></p> <p>70 Human T-Cell Lymphotropic Virus Types 1 and 2 674<br /><i>Breanna Caruso, Raya Massoud, and Steven Jacobson</i></p> <p>71 Diagnosis of Prion Diseases 682<br /><i>Richard Rubenstein, Robert B. Petersen, and Thomas Wisniewski</i></p> <p>72 Principles and Procedures of Human Immunodeficiency Virus Diagnosis 696<br /><i>Kelly A. Curtis, Jeffrey A. Johnson, and S. Michele Owen</i></p> <p><b>Section K Immunodeficiency Diseases 711</b></p> <p>Volume Editor: <i>Barbara Detrick</i></p> <p>Section Editors: <i>Kathleen E. Sullivan and Howard M. Lederman</i></p> <p>73 The Primary Immunodeficiency Diseases 713<br /><i>Howard M. Lederman</i></p> <p>74 Severe Combined Immune Deficiency: Newborn Screening 715<br /><i>James W. Verbsky and John M. Routes</i></p> <p>75 Combined Immunodeficiencies 721<br /><i>Christine Seroogy and Melissa Elder</i></p> <p>76 Antibody Deficiencies 737<br /><i>Kimberly C. Gilmour, Anita Chandra, and D. S. Kumararatne</i></p> <p>77 Hereditary and Acquired Complement Deficiencies 749<br /><i>Patricia C. Giclas</i></p> <p>78 Neutropenia and Neutrophil Defects 765<br /><i>Steven M. Holland</i></p> <p>79 Evaluation of Natural Killer (NK) Cell Defects 774<br /><i>Kimberly Risma and Rebecca Marsh</i></p> <p><b>Section L Allergic Diseases 781</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>Pamela A. Guerrerio</i></p> <p>80 Introduction 783<br /><i>Pamela A. Guerrerio</i></p> <p>81 Quantitation and Standardization of Allergens 784<br /><i>Ronald L. Rabin, Lynnsey Renn, and Jay E. Slater</i></p> <p>82 Immunological Methods in the Diagnostic Allergy Clinical and Research Laboratory 795<br /><i>Robert G. Hamilton</i></p> <p>83 Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation 801<br /><i>John T. Schroeder, R. Stokes Peebles, Jr., and Pamela A. Guerrerio</i></p> <p>84 Tests for Immunological Reactions to Foods 815<br /><i>Carah B. Santos, David M. Fleischer, and Robert A. Wood</i></p> <p>85 Diagnosis of Rare Eosinophilic and Mast Cell Disorders 825<br /><i>Cem Akin, Calman Prussin, and Amy D. Klion</i></p> <p><b>Section M Systemic Autoimmune Diseases 839</b></p> <p>Volume Editor: <i>Barbara Detrick</i></p> <p>Section Editor: <i>Westley H. Reeves</i></p> <p>86 Introduction 841<br /><i>Westley H. Reeves</i></p> <p>87 Antinuclear Antibody Tests 843<br /><i>Alessandra Dellavance, Wilson De Melo Cruvinel, Paulo Luiz Carvalho</i> <i>Francescantonio, and Luis Eduardo Coelho Andrade</i></p> <p>88 Detection of Autoantibodies by Enzyme-Linked Immunosorbent Assay and Bead Assays 859<br /><i>Edward K. L. Chan, Rufus W. Burlingame, and Marvin J. Fritzler</i></p> <p>89 Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus 868<br /><i>Westley Reeves, Shuhong Han, John Massini, and Yi Li</i></p> <p>90 Immunodiagnosis of Autoimmune Myopathies 878<br /><i>Minoru Satoh, Angela Ceribelli, Michito Hirakata, and Edward K. L. Chan</i></p> <p>91 Immunodiagnosis of Scleroderma 888<br /><i>Masataka Kuwana</i></p> <p>92 Antibody and Biomarker Testing in Rheumatoid Arthritis 897<br /><i>Ann Duskin Chauffe and Michael Raymond Bubb</i></p> <p>93 Antiphospholipid Antibody Syndrome: Clinical Manifestations and Laboratory Diagnosis 905<br /><i>Martina Murphy and Neil Harris</i></p> <p>94 Antineutrophil Cytoplasmic Antibodies (ANCA) and Strategies for Diagnosing ANCA-Associated Vasculitides 909<br /><i>R. W. Burlingame, C. E. Buchner, J. G. Hanly, and N. M. Walsh</i></p> <p>95 IgG4-Related Disease: Diagnostic Testing by Serology, Flow Cytometry, and Immunohistopathology 917<br /><i>John H. Stone</i></p> <p>96 Future Perspectives for Rheumatoid Arthritis and Other Autoimmune Diseases 922<br /><i>Jeremy Sokolove</i></p> <p><b>Section N Organ-localized Autoimmune Diseases 927</b></p> <p>Volume Editor: <i>John L. Schmitz</i></p> <p>Section Editors: <i>C. Lynne Burek and Patrizio Caturegli</i></p> <p>97 Introduction 929<br /><i>C. Lynne Burek</i></p> <p>98 Endocrinopathies: Chronic Thyroiditis, Addison Disease, Pernicious Anemia, Graves’ Disease, Diabetes, and Hypophysitis 930<br /><i>C. Lynne Burek, N. R. Rose, Giuseppe Barbesino, Jian Wang, Andrea K. Steck, George S. Eisenbarth, Liping Yu, Ludovica De Vincentiis, Adriana Ricciuti, Alessandra De Remigis, and Patrizio Caturegli</i></p> <p>99 Myasthenia Gravis 954<br /><i>Arnold I. Levinson and Robert P. Lisak</i></p> <p>100 Autoantibodies to Glycolipids in Peripheral Neuropathy 961<br /><i>Hugh J. Willison</i></p> <p>101 Detection of Antimitochondrial Autoantibodies in Primary Biliary Cholangitis and Liver Kidney Microsomal Antibodies in Autoimmune Hepatitis 966<br /><i>Patrick S. C. Leung, Michael P. Manns, Ross L. Coppel, and M. Eric Gershwin</i></p> <p>102 Cardiovascular Diseases 975<br /><i>Cheryl L. Maier, C. Lynne Burek, Noel R. Rose, and Aftab A. Ansari</i></p> <p>103 Celiac Disease and Inflammatory Bowel Disease 983<br /><i>Melissa R. Snyder</i></p> <p>104 Autoantibodies Directed against Erythrocytes in Autoimmune Hemolytic Anemia 990<br /><i>R. Sue Shirey and Karen E. King</i></p> <p>105 Immune Thrombocytopenia 995<br /><i>Thomas S. Kickler</i></p> <p>106 Monitoring Autoimmune Reactivity within the Retina 998<br /><i>John J. Hooks, Chi-Chao Chan, H. Nida Sen, Robert Nussenblatt, and Barbara Detrick</i></p> <p><b>Section O Cancer 1005</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editors: <i>Daniel Chan and Lori J. Sokoll</i></p> <p>107 Introduction 1007<br /><i>Robert G. Hamilton</i></p> <p>108 Immunoassay-Based Tumor Marker Measurement: Assays, Applications, and Algorithms 1008<br /><i>Elizabeth A. Godbey, Lori J. Sokoll, and Alex J. Rai</i></p> <p>109 Malignancies of the Immune System: Use of Immunologic and Molecular Tumor Markers in Classification and Diagnostics 1015<br /><i>Elaine S. Jaffe and Mark Raffeld</i></p> <p>110 Monitoring of Immunologic Therapies 1036<br /><i>Theresa L. Whiteside</i></p> <p>111 Circulating Tumor Cells as an Analytical Tool in the Management of Patients with Cancer 1051<br /><i>Daniel C. Danila, Howard I. Scher, and Martin Fleisher</i></p> <p><b>Section P Transplantation Immunology 1063</b></p> <p>Volume Editor: <i>Barbara Detrick</i></p> <p>Section Editors: <i>Elaine F. Reed and Qiuheng Jennifer Zhang</i></p> <p>112 Histocompatibility and Immunogenetics Testing in the 21st Century 1065<br /><i>Qiuheng Jennifer Zhang and Elaine F. Reed</i></p> <p>113 Molecular Methods for Human Leukocyte Antigen Typing: Current Practices and Future Directions 1069<br /><i>Mark Kunkel, Jamie Duke, Deborah Ferriola, Curt Lind, and Dimitri Monos</i></p> <p>114 Evaluation of the Humoral Response in Transplantation 1091<br /><i>Paul Sikorski, Renato Vega, Donna P. Lucas, and Andrea A. Zachary</i></p> <p>115 Non-Human Leukocyte Antigen Antibodies in Organ Transplantation 1103<br /><i>Annette M. Jackson and Bethany L. Dale</i></p> <p>116 Evaluation of the Cellular Immune Response in Transplantation 1108<br /><i>Diana Metes, Nancy L. Reinsmoen, and Adriana Zeevi</i></p> <p>117 Complement in Transplant Rejection 1123<br /><i>Carmela D. Tan, E. Rene Rodriguez, and William M. Baldwin Iii</i></p> <p>118 Molecular Characterization of Rejection in Solid Organ Transplantation 1132<br /><i>Darshana Dadhania, Tara K. Sigdel, Thangamani Muthukumar, Choli Hartono, Minnie M. Sarwal, and Manikkam Suthanthiran</i></p> <p>119 Killer Cell Immunoglobulin-Like Receptors in Clinical Transplantation 1150<br /><i>Raja Rajalingam, Sarah Cooley, and Jeroen Van Bergen</i></p> <p>120 Chimerism Testing 1161<br /><i>Lee Ann Baxter-Lowe</i></p> <p><b>Section Q Laboratory Management 1169</b></p> <p>Volume Editor: <i>Robert G. Hamilton</i></p> <p>Section Editor: <i>Ronald J. Harbeck</i></p> <p>121 Clinical Immunology Laboratory Accreditation, Licensure, and Credentials 1171<br /><i>Linda Cook and Ronald J. Harbeck</i></p> <p>122 Validation and Quality Control: General Principles and Application to the Clinical Immunology Laboratory 1180<br /><i>Vijaya Knight and Terri Lebo</i></p> <p>Author Index 1193</p> <p>Subject Index 1195</p>
<p>Description</p> <p>This eighth edition of a manual for laboratory immunology comes 10 years after the 2006 edition. It is available in a print version (5.8 pounds, 1,245 pages) and an electronic version (EPUB or PDF).</p> <p>Purpose</p> <p>The purpose is to provide a single, authoritative reference source for laboratory immunology. This is a most worthy objective, well met by previous editions and, true to its reputation of excellence, this edition meets it as well. The electronic versions are welcome enhancements, permitting easy portability and access and accessibility options (e.g., font size, contrast, etc.).</p> <p>Audience</p> <p>It is intended for those who work in the clinical immunology laboratory - from clinical laboratory scientists in training or practice, to residents or fellows in clinical immunology or laboratory medicine/clinical pathology, to attendings in practice in either discipline, and especially laboratory directors. This book would also be of interest to clinicians in the many internal medicine subspecialties treating patients with diseases with immunological pathogenesis, to better understand and therefore more appropriately use testing for diagnosis, treatment, and management.</p> <p>Features</p> <p>It has been 10 years since the seventh edition, which also incorporated "molecular" into the title for the first time. The revolution in the past decade in molecular-based testing methods has exploded, so this edition is much needed and welcomed. The first seven sections are devoted to general methodologies, the next nine to diseases, and the last section to laboratory management. The molecular aspects have been deftly interwoven - whether proteomic techniques, the role of the microbiome in disease, HLA typing with molecular methods, immunoglobulin gene rearrangement, or transplantation immunology, etc. There are a few omissions. Many of the disease-specific chapters contain experimental protocols, but surprisingly many lacked discussions of quality controls. Some laboratory protocols are casually referred to as "laboratory developed tests" (LDTs). LDTs have been a most controversial issue in the past few years, yet this book devotes only a single paragraph in the laboratory management chapter on the proposed regulatory requirements. While the references cited in this paragraph would provide more-than-you-could-ever-want-to-know detail about Food and Drug Administration (FDA) considerations for LDT regulation, the controversy and therefore immediate risks to accreditation and licensure are not obvious to the naive reader. An enhancement for the next edition would be cautionary alerts to readers concerning implementation of LDTs, and inclusion of quality control testing instructions for every experimental protocol.</p> <p>Assessment</p> <p>This is THE authoritative reference for molecular and clinical laboratory immunology. If you perform this testing or want an expert update in the field, you will want this book.</p> <p>Weighted Numerical Score: 91 - 4 Stars!</p> <b>-Valerie L. Ng, PhD, MD,</b> (Alameda County Medical Center/Highland Hospital)
<p><b>Manual of Molecular and Clinical Laboratory Immunology</b> <p>Eighth Edition <p><i>THE authoritative guide for clinical laboratory immunology</i> <p>For over 40 years the <i>Manual of Molecular and Clinical Laboratory Immunology</i> has served as the premier guide for the clinical immunology laboratory. From basic serology testing to the present wide range of molecular analyses, the Manual has reflected the exponential growth in the field of immunology over the past decades. This eighth edition reflects the latest advances and developments in the diagnosis and treatment of patients with infectious and immune-mediated disorders. The Manual features detailed descriptions of general and specific methodologies, placing special focus on the interpretation of laboratory findings, and covers the immunology of infectious diseases, including specific pathogens, as well as the full range of autoimmune and immunodeficiency diseases, cancer, and transplantation. <p>Written to guide the laboratory director, the Manual will also appeal to other laboratory scientists, especially those working in clinical immunology laboratories, and pathologists. It is also a useful reference for physicians, mid-level providers, medical students, and allied health students with an interest in the role that immunology plays in the clinical laboratory.

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