Details

MALDI-TOF and Tandem MS for Clinical Microbiology


MALDI-TOF and Tandem MS for Clinical Microbiology


1. Aufl.

von: Haroun N. Shah, Saheer E. Gharbia

139,99 €

Verlag: Wiley
Format: PDF
Veröffentl.: 30.03.2017
ISBN/EAN: 9781118960240
Sprache: englisch
Anzahl Seiten: 648

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Beschreibungen

<p>This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade.  This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss:<br />Challenges of Identifying Mycobacterium to the Species level <br />Identification of <i>Bacteroides</i> and Other Clinically Relevant Anaerobes<br />Identification of Species in Mixed Microbial Populations<br />Detection of Resistance Mechanisms<br />Proteomics as a biomarker discovery and validation platform<br />Determination of Antimicrobial Resistance using Tandem Mass Spectrometry</p>
<p>List of Contributors xxi</p> <p>Preface xxix</p> <p><b>Part I MALDI-TOF Mass Spectrometry 1</b></p> <p>1 A Paradigm Shift from Research to Front?]Line Microbial Diagnostics in MALDI?]TOF and LC?]MS/MS: A Laboratory’s Vision and Relentless Resolve to Help Develop and Implement This New Technology amidst Formidable Obstacles 3<br /><i>Haroun N. Shah and Saheer E. Gharbia</i></p> <p>2 Criteria for Development of MALDI?]TOF Mass Spectral Database 39<br /><i>Markus Kostrzewa and Thomas Maier</i></p> <p>3 Applications of MALDI?]TOF Mass Spectrometry in Clinical Diagnostic Microbiology 55<br /><i>Onya Opota, Guy Prod’hom and Gilbert Greub</i></p> <p>4 The Challenges of Identifying Mycobacterium to the Species Level using MALDI?]TOF MS 93</p> <p>5 Transformation of Anaerobic Microbiology since the Arrival of MALDI?]TOF Mass Spectrometry 123<br /><i>Elisabeth Nagy, Mariann Abrok, Edith Urban, A.C.M. Veloo, Arie Jan van Winkelhoff, Itaru Dekio, Saheer E. Gharbia and Haroun N. Shah</i></p> <p>6 Differentiation of Closely Related Organisms using MALDI?]TOF MS 147<br /><i>Mark A. Fisher</i></p> <p>7 Identification of Species in Mixed Microbial Populations using MALDI?]TOF MS 167<br /><i>Pierre Mahe, Maud Arsac, Nadine Perrot, Marie?]Helene Charles, Patrick Broyer, Jay Hyman, John Walsh, Sonia Chatellier, Victoria Girard, Alex van Belkum, and Jean?]Baptiste Veyrieras</i></p> <p>8 Microbial DNA Analysis by MALDI?]TOF Mass Spectrometry 187</p> <p>9 Impact of MALDI?]TOF MS in Clinical Mycology; Progress and Barriers in Diagnostics 211<br /><i>Cledir R. Santos, Elaine Francisco, Mariana Mazza, Ana Carolina B. Padovan, Arnaldo Colombo and Nelson Lima</i></p> <p>10 Development and Application of MALDI?]TOF for Detection of Resistance Mechanisms 231<br /><i>Stefan Zimmermann and Irene Burckhardt</i></p> <p>11 Discrimination of Burkholderia Species, Brucella Biovars, Francisella tularensis and Other Taxa at the Subspecies Level by MALDI?]TOF Mass Spectrometry 249<br /><i>Axel Karger</i></p> <p>12 MALDI?]TOF?]MS Based on Ribosomal Protein Coding in S10?]spc?]alpha Operons for Proteotyping 269<br /><i>Hiroto Tamura</i></p> <p><b>Part II Tandem MS/MS-Based Approaches to Microbial Characterization 311</b></p> <p>13 Tandem Mass Spectrometry Analysis as an Approach to Delineate Genetically Related Taxa 313<br /><i>Raju V. Misra, Tom Gaulton, Nadia Ahmod, Min Fang, Martin Hornshaw, Jenny Ho, Saheer E. Gharbia and Haroun N. Shah</i></p> <p>14 Mapping of the Proteogenome of Clostridium difficile Isolates of Varying Virulence 379<br /><i>Caroline H. Chilton, Saheer E. Gharbia, Raju V. Misra, Min Fang, Ian R. Poxton, Peter S. Borriello and Haroun N. Shah</i></p> <p>15 Determination of Antimicrobial Resistance using Tandem Mass<br />Spectrometry 399<br /><i>Ajit J. Shah, Vlad Serafim, Zhen Xu, Hermine Mkrtchyan and Haroun N. Shah</i></p> <p>16 Proteotyping: Tandem Mass Spectrometry Shotgun Proteomic Characterization and Typing of Pathogenic Microorganisms 419<br /><i>Roger Karlsson, Lucia Gonzales?]Siles, Fredrik Boulund, Asa Lindgren, Liselott Svensson?]Stadler, Anders Karlsson, Erik Kristiansson and Edward R.B. Moore</i></p> <p>17 Proteogenomics of Pseudomonas aeruginosa in Cystic Fibrosis Infections 451<br /><i>Liang Yang and Song Lin Chua</i></p> <p>18 Top?]Down Proteomics in the Study of Microbial Pathogenicity 493<br /><i>Joseph Gault, Egor Vorontsov, Mathieu Dupre, Valeria Calvaresi, Magalie Duchateau, Diogo B. Lima, Christian Malosse and Julia Chamot?]Rooke</i></p> <p>19 Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions 505<br /><i>Carolin Kolmeder, Kaarina Lahteenmaki, Pirjo Wacklin, Annika Kotovuori, Ilja Ritamo, Jaana Matto, Willem M. de Vos, and Leena Valmu</i></p> <p>20 Proteogenomics of Non?]model Microorganisms 529<br /><i>Jean Armengaud</i></p> <p>21A Analysis of MALDI?]TOF MS Spectra using the BioNumerics Software 539<br /><i>Katleen Vranckx, Katrien De Bruyne and Bruno Pot</i></p> <p>21B Subtyping of Staphylococcus spp. Based upon MALDI?]TOF MS Data Analysis 563<br /><i>Zhen Xu, Ali Olkun, Katleen Vranckx, Hermine V. Mkrtchyan, Ajit J. Shah, Bruno Pot, Ronald R. Cutler and Haroun N. Shah</i></p> <p>21C Elucidating the Intra?]Species Proteotypes of Pseudomonas aeruginosa from Cystic Fibrosis 579<br /><i>Ali Olkun, Ajit J. Shah and Haroun N. Shah</i></p> <p>References 588</p> <p>Index 593</p> <p> </p>
<p><b> EDITORS<br> Haroun N. Shah,</b> Department of Natural Sciences, Middlesex University, London, UK <p><b> Saheer E. Gharbia,</b> Genomic Research, Public Health England, London, UK
<p>This comprehensive handbook highlights the triumph and developments of MALDI-TOF mass spectrometry in diagnostic approaches to clinical microbiology over the past decade, and provides insight into new technologies including tandem MS/MS. Presenting the reader with detailed coverage of current research and future opportunities, this volume, divided into two parts, with 21 chapters, includes such topics as:</p> <ul> <li>Applications of MALDI-TOF Mass Spectrometry in Clinical Diagnostic Microbiology</li> <li>The Potential of MALDI-TOF MS to Distinguish between Taxonomically Similar Species that Manifest Different Infections</li> <li>Microbial DNA Analysis by MALDI-TOF Mass Spectrometry</li> <li>Development and Application of MALDI-TOF MS for Detection of Resistance Mechanisms</li> <li>Determination of Antimicrobial Resistance using Tandem Mass Spectrometry</li> <li>Top-Down Proteomics in the Study of Microbial Pathogenicity</li> <li>Proteogenomics of Non-Model Microorganisms</li> </ul> <p>Primarily aimed at clinical microbiologists, diagnostic technologists, biomedical scientists, researchers, clinicians, academics and scientists in bio-resource centres, this book is also relevant to researchers, post-graduate scientists, technologists in the food industry, pharmaceuticals and agriculture.</p>

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