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<p>Preface ix</p> <p>Acknowledgements xi</p> <p><b>1 Introduction 1</b></p> <p>1.1 What Are the Tasks in Proteomics? 1</p> <p>1.2 Challenges in Proteomics 5</p> <p>1.3 Proteomics in Relation to Other -omics and System Biology 10</p> <p>1.4 Some General Applications of Proteomics 12</p> <p>1.5 Structure of the Book 18</p> <p>References 18</p> <p><b>2 Separation and Detection Technologies 21</b></p> <p>2.1 Introduction to Experimental Strategies in Proteomics 21</p> <p>2.2 Gel-Based Separation 31</p> <p>2.3 Visualization and Analysis of Proteins/Peptides in Gels 40</p> <p>2.4 Gel-Free Separation Technologies 54</p> <p>2.5 Visualization of Proteins/Peptides from Hyphenated Methods 74</p> <p>2.6 Chips in Proteomic Applications 81</p> <p>References 81</p> <p><b>3 Analysis of Peptides/Proteins by Mass Spectrometry 83</b></p> <p>3.1 Basic Principles of Mass Spectrometry for Proteomics 83</p> <p>3.2 Ionization Methods for Small Amounts of Biomolecules 101</p> <p>3.3 Mass Analyzers and Mass Spectrometers 116</p> <p>3.4 Concluding Remarks on Mass Analyzers for Proteomics 170</p> <p>References 170</p> <p><b>4 Analysis and Interpretation of Mass Spectrometric and Proteomic Data 173</b></p> <p>4.1 Introduction 173</p> <p>4.2 Analysis of MS Data 174</p> <p>4.3 Analysis of MS/MS Data 192</p> <p>4.4 Quantification of LC MS and MS/MS Data from Complex Samples 209</p> <p>4.5 Bioinformatic Approaches for Mass Spectrometric Proteome Data Analysis 213</p> <p>References 218</p> <p><b>5 Strategies in Proteomics 221</b></p> <p>5.1 Imaging Mass Spectrometry 221</p> <p>5.2 Qualitative Proteomics 223</p> <p>5.3 Differential and Quantitative Proteomics 234</p> <p>5.4 Analysis of Posttranslational Modifications 257</p> <p>5.5 Interaction Proteomics 261</p> <p>5.6 Proteomics as Part of Integrated Approaches 266</p> <p>References 271</p> <p>Index 275</p>

"He introduces undergraduate students to the general principles and methods of the field without delving very deeply into any of the details. Graduate students and researchers could also use the book to refresh their memory or catch up with recent developments." (Booknews, 1 June 2011)

<p><strong>Josip Lovric</strong> is the author of <em>Introducing Proteomics: From Concepts to Sample Separation, Mass Spectrometry and Data Analysis</em>, published by Wiley.

<i>Introducing Proteomics</i> provides an accessible introduction to current proteomics technology, including all of the background information necessary to enable readers to plan, prepare and analyze proteomics studies. Engaging and packed with expert advice, this indispensable guide will appeal to both students and researchers new to the field alike. <p>Key Features:</p> <ul> <li> <div><b>Contemporary, broad coverage</b> – covers every important aspect of this rapidly changing field from 2-d gel electrophoretic methods, peptide based shotgun proteomics, the main ionization methods and detailed mass spectrometric principles and applications as well as an introduction to the use of protein/RNA and DNA databases to data analysis and the main features of proteomics data mining software.</div> </li> <li> <div><b>Coherent and student friendly</b> – focuses on what readers need to know about each area of proteomics whilst providing pointers towards more specialized literature for those that are keen to find out more.</div> </li> <li> <div><b>Relevant</b> – explores the power and limitations of current proteomics approaches throughout the book to give readers a realistic idea about which results and challenges to expect from any combination of technologies. The concluding part of the book explores real-world case studies to illustrate how proteomics technologies perform in practice whilst dedicated chapters focus on the analysis of post translational modifications and biomarker discovery.</div> </li> <li> <div><b>Companion website</b> – provides a range of problems for self testing with answers, extensive references and links to additional, key proteomics resources.</div> </li> </ul> <p><b><i>‘I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues.’</i></b> – Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin</p> <p><b>Companion Website</b></p> <p><a href="http://www.wiley.com/go/lovric">www.wiley.com/go/lovric</a></p>

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