Gradient HPLC for PractitionersRP, LC-MS, Ion Analytics, Biochromatography, SFC, HILIC
This practical guide for analytical scientists explains the use of gradients in liquid chromatography. The fundamentals of gradient separations, as well as the most common application scenarios are addressed, from LC-MS coupling to biochromatography to the separation of ionic substances. Throughout, this handy volume provides detailed hands-on information for practitioners, enabling them to use gradient separation methods reliably and efficiently.
Aspects of Gradient Optimization Prediction of Gradients The Gradient; Requirements, Optimal Use, Hints, and Pitfalls Use of Gradients in LC-MS Specifications of Gradients in Biochromatography The Gradient in the Separation of Ionic Components Use of Gradients in Hydrophilic Interaction Liquid Chromatography (HILIC) Specifications of Gradients in Supercritical Fluid Chromatography (SFC)
Stavros Kromidas, PhD, works as an independent consultant for analytical chemistry, based in Saarbrucken (Germany). For more than 20 years he has regularly held lectures and training courses on HPLC, and has authored numerous articles and several books on various aspects of chromatography. He is the founder of NOVIA GmbH, a provider of professional training and consulting in analytical chemistry, and served as its CEO until 2001.
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