Details

<p>Preface ix</p> <p>Preface to the First Edition xi</p> <p>Acknowledgments xiii</p> <p>Chapter 1 Designer Mice 1<br /><i>Scope and sourcebooks</i></p> <p>Chapter 2 Of Unicorns and Chimeras 10<br /><i>How to generate a line of transgenic or knockout mouse for behavioural phenotyping</i></p> <p>Chapter 3 General Health 42<br /><i>Give your mouse a physical</i></p> <p>Chapter 4 Motor Functions 62<br /><i>Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures</i></p> <p>Chapter 5 Sensory Abilities 86<br /><i>Olfaction, vision, hearing, taste, touch, nociception</i></p> <p>Chapter 6 Learning and Memory 110<br /><i>Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention</i></p> <p>Chapter 7 Feeding and Drinking 164<br /><i>Daily consumption, restricted access, choice tests, microstructural analysis</i></p> <p>Chapter 8 Reproductive Behaviors 186<br /><i>Sexual and parental behaviors</i></p> <p>Chapter 9 Social Behaviors 206<br /><i>Social interaction, nesting, grooming, juvenile play, aggression</i></p> <p>Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226<br /><i>Mouse models of fear, anxiety, depression, schizophrenia</i></p> <p>Chapter 11 Reward 266<br /><i>Self-administration of addictive drugs, conditioned place preference</i></p> <p>Chapter 12 Neurodevelopment and Neurodegeneration 290<br /><i>Assaying behaviors in infant, juvenile, and aged mice</i></p> <p>Chapter 13 Putting It All Together 322<br /><i>Choice of tests, order of testing, number of mice, equipment, housing and testing environment</i></p> <p>Chapter 14 The Next Generation 344<br /><i>Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues</i></p> <p>References 383</p> <p>Index 503</p>

<b>First Edition Reviews:</b> <p>"The book successfully brings together the field of behavioral neuroscience and the rewards and insights that can be gained using this approach. I would recommend that every behavioral scientist has at least two copies, one for their own use and one that will be on permanent load to their students, post-doctoral students and colleagues in molecular biology." (<i>Trends in Pharmaceutical Sciences</i>)</p> <p>"the overall impression is of a helpful guide taking the reader step by step through the complexities of animal behavioral research." (<i>Nature Neuroscience</i>)</p> <p>"succeeds as a useful introduction to the realm of behavioral phenotyping for those interested in creating or using the large and increasing number of promising targeted mutant mice." (<i>Trends in Neurosciences</i>)</p>

<b>Jacqueline N. Crawley</b>, PhD, is Chief of the Laboratory of Behavioral Neuroscience at the National Institute of Mental Health; Adjunct Professor of Psychiatry and Psychology at the University of North Carolina at Chapel Hill; and Adjunct Professor of Pharmacology at Georgetown University. Dr. Crawley has published more than 200 peer-reviewed papers and 60 review articles. Her research has included rodent behavioral analyses of noradrenergic pathways; benzodiazepine receptor ligands; neuropeptide transmitters, including galanin and cholecystokinin; mouse genetic models of autism; and behavioral phenotyping of more than 30 lines of transgenic and knockout mice. Recent awards include the International Behavioral Neuroscience Society Myers Lifetime Achievement Award (2005), the Fleur Strand Summer Neuropeptide Conference Award (2005), the Howard Hughes Medical Institute Preceptor Award (2004), and the National Institute of Mental Health Director's Award (2002). <p>What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice, Second Edition has been written in Dr. Crawley's private capacity, outside of her professional position at the National Institutes of Health. The views expressed in this book do not necessarily represent the views of the National Institutes of Health or of the United States government.</p>

<b>Learn how to conduct behavioral tests to phenotype mutant mice</b> <p>Dr. Jacqueline N. Crawley, author of the first and second editions of <i>What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice</i>, continues to field hundreds of calls and e-mails from molecular geneticists who ask just that: How do I run behavioral assays to find out what's wrong with my mouse? This book answers that question and many more, including what tests are available to discover an aberrant behavioral phenotype?</p> <p>Turn to <i>What's Wrong With My Mouse?</i> to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the second edition up to date with the latest science. In addition, the second edition includes two new chapters:</p> <ul> <li> <p>Neurodevelopment and Neurodegeneration discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimer's, Parkinson's, Huntington's, and amyotrophic lateral sclerosis.</p> </li> <li> <p>Putting It All Together recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.</p> </li> </ul> <p>The final chapter, The Next Generation, examines new and emerging technologies.</p> <p>Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor Web sites, suggestions for expert behavioral neuroscientist collaborators, and an extensive list of references, make this book a valuable and practical resource for students and researchers.</p>

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