Table of Contents
Cover
Related Titles
Title Page
Copyright
Dedication
List of Contributors
About the Series Editors
Preface
Part I: Enabling and Improving Large-Scale Bio-production
Chapter 1: Industrial-Scale Fermentation
1.1 Introduction
1.2 Industrial-Scale Fermentation Today
1.3 Engineering and Design Aspects
1.4 Industrial Design Examples
1.5 Cost Analysis for the Manufacture of Biotechnological Products
1.6 Influence of Process- and Facility-Related Aspects on Cost Structure
Acknowledgments
References
Chapter 2: Scale-Down: Simulating Large-Scale Cultures in the Laboratory
2.1 Introduction
2.2 Heterogeneities at Large Scale and the Need for Scaling Down
2.3 Bioreactor Scale-Down
2.4 Tools to Study Cell Responses to Environmental Heterogeneities
2.5 Physiological Effects of Environmental Heterogeneities
2.6 Improvements Based on Scale-Down Studies: Bioreactor Design and Cell Engineering
2.7 Perspectives
Acknowledgment
References
Chapter 3: Bioreactor Modeling
3.1 Large-Scale Industrial Fermentations: Challenges for Bioreactor Modeling
3.2 Bioreactors
3.3 Compartment and Hybrid Multizonal/Computational Fluid Dynamics Approaches for the Description of Large-Scale Bioreactor Phenomena
3.4 Computational Fluid Dynamics Modeling: Unstructured Continuum Approach (Euler–Euler)
3.5 Computational Fluid Dynamics Modeling: Structured Segregated Approach (Euler–Lagrange)
3.6 Conclusion
3.7 Outlook
References
Chapter 4: Cell Culture Technology
4.1 Introduction
4.2 Overview of Applications for Cell Culture Products and Tissue Engineering
4.3 Fundamentals
4.4 Bioreactors for Cell Culture
4.5 Downstream
4.6 Regulatory and Safety Issues
4.7 Conclusions and Outlook
References
Part II: Getting Out More: Strategies for Enhanced Bioprocessing
Chapter 5: Production of Fuels and Chemicals from Biomass by Integrated Bioprocesses
5.1 Introduction
5.2 Utilization of Starchy Biomass
5.3 Utilization of Lignocellulosic Biomass
5.4 Conclusions and Perspectives
Acknowledgment
References
Chapter 6: Solid-State Fermentation
6.1 Introduction
6.2 Fundamentals Aspects of SSF
6.3 Factors Affecting Solid-State Fermentation
6.4 Scale-Up
6.5 Product Recovery
6.6 Bioreactor Designing
6.7 Kinetics and Modeling
6.8 Applications
6.9 Challenges in SSF
6.10 Summary
References
Chapter 7: Cell Immobilization: Fundamentals, Technologies, and Applications
7.1 Introduction
7.2 Fundamentals of Cell Immobilization
7.3 Immobilization with Support Materials
7.4 Self-Immobilization
7.5 Immobilized Cells and their Applications
7.6 Bioreactors for Cell Immobilization
7.7 Challenges and Recommendations for Future Research
7.8 Conclusions
References
Part III: Molecules for Human Use: High-Value Drugs, Flavors, and Nutraceuticals
Chapter 8: Anticancer Drugs
8.1 Natural Products as Anticancer Drugs
8.2 Anticancer Drug Production
8.3 Important Anticancer Natural Products
8.4 Prospects
References
Chapter 9: Biotechnological Production of Flavors
9.1 History
9.2 Survey on Today's Industry
9.3 Regulations
9.4 Flavor Production
9.5 Biotechnological Production of Flavors
9.6 Vanillin
9.7 2-Phenylethanol
9.8 Benzaldehyde
9.9 Lactones
9.10 Raspberry Ketone
9.11 Green Notes
9.12 Nootkatone
9.13 Future Perspectives
References
Chapter 10: Nutraceuticals (Vitamin C, Carotenoids, Resveratrol)
10.1 Introduction
10.2 Vitamin C
10.3 Carotenoids
10.4 Resveratrol
10.5 Future Perspectives
References
Part IV: Industrial Amino Acids
Chapter 11: Glutamic Acid Fermentation: Discovery of Glutamic Acid-Producing Microorganisms, Analysis of the Production Mechanism, Metabolic Engineering, and Industrial Production Process
11.1 Introduction
11.2 Discovery of the Glutamic Acid-Producing Bacterium
C. glutamicum
11.3 Analysis of the Mechanism of Glutamic Acid Production by
C. glutamicum
11.4 Metabolic Engineering of
C. glutamicum
for Glutamic Acid Production
11.5 Glutamic Acid Fermentation by Other Microorganisms
11.6 Industrial Process of Glutamic Acid Production
11.7 Future Perspectives
References
Chapter 12: l-Lysine
12.1 Uses of l-Lysine
12.2 Biosynthesis and Production of l-Lysine
12.3 The Chassis Concept: Biotin Prototrophy and Genome Reduction
12.4 l-Lysine Biosensors for Strain Selection and on-Demand Flux Control
12.5 Perspective
References
Part V: Bio-Based Monomers and Polymers
Chapter 13: Diamines for Bio-Based Materials
13.1 Introduction
13.2 Diamine Metabolism in Bacteria
13.3 Putrescine – 1,4-Diaminobutane
13.4 Cadaverine – 1,5-Diaminopentane
13.5 Conclusions and Perspectives
References
Chapter 14: Microbial Production of 3-Hydroxypropionic Acid
14.1 Introduction
14.2 3-HP Obtained from Native Producers
14.3 Synthesis of 3-HP from Glucose
14.4 Synthesis of 3-HP from Glycerol
14.5 Bridging the Gap Between Glucose and Glycerol in 3-HP Production
14.6 Other Strains for 3-HP Production from Glycerol
14.7 Limitations of 3-HP Synthesis
14.8 Conclusions and Future Prospects
Acknowledgments
References
Chapter 15: Itaconic Acid – An Emerging Building Block
15.1 Background, History, and Economy
15.2 Biosynthesis of Itaconic Acid
15.3 Production Conditions for Itaconic Acid
15.4 Physiological Effects and Metabolism of Itaconic acid
15.5 Metabolic Engineering for Itaconic Acid Production
15.6 Outlook
Acknowledgments
References
Part VI: Top-Value Platform Chemicals
Chapter 16: Microbial Production of Isoprene: Opportunities and Challenges
16.1 Introduction
16.2 The Milestones of Isoprene Production
16.3 Microbial Production of Isoprene: Out of the Laboratory
16.4 Main Challenges for Bioisoprene Production
16.5 Future Prospects
Acknowledgments
References
Chapter 17: Succinic Acid
17.1 Introduction
17.2 Development of Succinic Acid Producers and Fermentation Strategies
17.3 Succinic Acid Recovery and Purification
17.4 Summary
Acknowledgments
References
Part VII: Biorenewable Fuels
Chapter 18: Ethanol: A Model Biorenewable Fuel
18.1 Introduction
18.2 Metabolic Engineering: Design, Build, Test, Learn
18.3 Biomass Deconstruction
18.4 Closing Remarks
Acknowledgments
References
Chapter 19: Microbial Production of Butanols
19.1 Introduction
19.2 A Historical Perspective of
n
-Butanol Production
19.3 ABE Fermentation
19.4
n
-Butanol Production in Non-native Producers
19.5 Isobutanol Production
19.6 Summary and Outlook
Acknowledgments
References
Index
End User License Agreement
Pages
xxi
xxii
xxiii
xxiv
xxv
xxvi
xxvii
xxviii
xxix
xxx
xxxi
xxxii
xxxiii
xxxiv
1
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
597
598
599
600
601
602
603
604
605
Guide
Cover
Table of Contents
Preface
Part I: Enabling and Improving Large-Scale Bio-production
Begin Reading