Table of Contents
Cover
Title Page
Copyright
Dedication
Foreword
Preface
Abbreviations
Part I: Introduction
Chapter 1: Lipids and Lipidomics
1.1 Lipids
1.2 Lipidomics
References
Chapter 2: Mass Spectrometry for Lipidomics
2.1 Ionization Techniques
2.2 Mass Analyzers
2.3 Detector
2.4 Tandem Mass Spectrometry Techniques
2.5 Other Recent Advances in Mass Spectrometry for Lipid Analysis
References
Chapter 3: Mass Spectrometry-Based Lipidomics Approaches
3.1 Introduction
3.2 Shotgun Lipidomics: Direct Infusion-Based Approaches
3.3 LC-MS-Based Approaches
3.4 MALDI-MS for Lipidomics
References
Chapter 4: Variables in Mass Spectrometry for Lipidomics
4.1 Introduction
4.2 Variables in Lipid Extraction (i.e., Multiplex Extraction Conditions)
4.3 Variables in the Infusion Solution
4.4 Variables in Ionization
4.5 Variables in Building-Block monitoring with MS/MS Scanning
4.6 Variables in Collision
4.7 Variables in Separation
4.8 Conclusion
References
Chapter 5: Bioinformatics in Lipidomics
5.1 Introduction
5.2 Lipid Libraries and Databases
5.3 Bioinformatics Tools in Automated Lipid Data Processing
5.4 Bioinformatics for Lipid Network/Pathway Analysis and Modeling
5.5 Integration of “Omics”
References
Part II: Characterization of Lipids
Chapter 6: Introduction
6.1 Structural Characterization for Lipid Identification
6.2 Pattern Recognition for Lipid Identification
References
Chapter 7: Fragmentation Patterns Of Glycerophospholipids
7.1 Introduction
7.2 Choline Glycerophospholipid
7.3 Ethanolamine Glycerophospholipid
7.4 Phosphatidylinositol and Phosphatidylinositides
7.5 Phosphatidylserine
7.6 Phosphatidylglycerol
7.7 Phosphatidic Acid
7.8 Cardiolipin
7.9 Lysoglycerophospholipids
7.10 Other Glycerophospholipids
References
Chapter 8: Fragmentation Patterns of Sphingolipids
8.1 Introduction
8.2 Ceramide
8.3 Sphingomyelin
8.4 Cerebroside
8.5 Sulfatide
8.6 Oligoglycosylceramide and Gangliosides
8.7 Inositol Phosphorylceramide
8.8 Sphingolipid Metabolites
References
Chapter 9: Fragmentation Patterns of Glycerolipids
9.1 Introduction
9.2 Monoglyceride
9.3 Diglyceride
9.4 Triglyceride
9.5 Hexosyl Diacylglycerol
9.6 Other Glycolipids
References
Chapter 10: Fragmentation Patterns of Fatty Acids and Modified Fatty Acids
10.1 Introduction
10.2 Nonesterified Fatty Acid
10.3 Modified Fatty Acid
10.4 Fatty Acidomics
References
Chapter 11: Fragmentation Patterns of other Bioactive Lipid Metabolites
11.1 Introduction
11.2 Acylcarnitine
11.3 Acyl CoA
11.4 Endocannabinoids
11.5 4-Hydroxyalkenal
11.6 Chlorinated Lipids
11.7 Sterols and Oxysterols
11.8 Fatty Acid–Hydroxy Fatty Acids
References
Chapter 12: Imaging Mass Spectrometry of Lipids
12.1 Introduction
12.2 MALDI-MS Imaging
12.3 Secondary-Ion Mass Spectrometry Imaging
12.4 DESI-MS Imaging
12.5 Ion-Mobility Imaging
12.6 Advantages and Drawbacks of Imaging Mass Spectrometry for Analysis of Lipids
References
Part III: Quantification of Lipids in Lipidomics
Chapter 13: Sample Preparation
13.1 Introduction
13.2 Sampling, Storage, and Related Concerns
13.3 Principles and Methods of Lipid Extraction
References
Chapter 14: Quantification of Individual Lipid Species in Lipidomics
14.1 Introduction
14.2 Principles of Quantifying Lipid Species by Mass Spectrometry
14.3 Methods for Quantification in Lipidomics
References
Chapter 15: Factors Affecting Accurate Quantification of Lipids
15.1 Introduction
15.2 Lipid Aggregation
15.3 Linear Dynamic Range of Quantification
15.4 Nuts and Bolts of Tandem Mass Spectrometry for Quantification of Lipids
15.5 Ion Suppression
15.6 Spectral Baseline
15.7 The Effects of Isotopes
15.8 Minimal Number of Internal Standards for Quantification
15.9 In-Source Fragmentation
15.10 Quality of Solvents
15.11 Miscellaneous in Quantitative Analysis of Lipids
References
Chapter 16: Data Quality Control and Interpretation
16.1 Introduction
16.2 Data Quality Control
16.3 Recognition of Lipid Metabolism Pathways for Data Interpretation
16.4 Recognition of Lipid Functions for Data Interpretation
16.5 Recognizing the Complication of Sample Inhomogeneity and Cellular Compartments in Data Interpretation
16.6 Integration of “Omics” for Data Supporting
References
Part IV: Applications of Lipidomics in Biomedical and Biological Research
Chapter 17: Lipidomics for Health and Disease
17.1 Introduction
17.2 Diabetes and Obesity
17.3 Cardiovascular Diseases
17.4 Nonalcohol Fatty Liver Disease
17.5 Alzheimer's disease
17.6 Psychosis
17.7 Cancer
17.8 Lipidomics in Nutrition
References
Chapter 18: Plant Lipidomics
18.1 Introduction
18.2 Characterization of Lipids Special to Plant Lipidome
18.3 Lipidomics for Plant Biology
References
Chapter 19: Lipidomics on Yeast and Mycobacterium Tuberculosis
19.1 Introduction
19.2 Yeast Lipidomics
19.3
Mycobacterium Tuberculosis
Lipidomics
References
Chapter 20: Lipidomics on Cell Organelle and Subcellular Membranes
20.1 Introduction
20.2 Golgi
20.3 Lipid Droplets
20.4 Lipid Rafts
20.5 Mitochondrion
20.6 Nucleus
20.7 Conclusion
References
Index
Wiley Series on Mass Spectrometry
End User License Agreement
Pages
xix
xx
xxi
xxii
xxiii
xxv
xxvi
xxvii
xxviii
1
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
151
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
281
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
377
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
Guide
Table of Contents
Begin Reading