TABLE OF CONTENTS
TITLE PAGE
COPYRIGHT
LIST OF CONTRIBUTORS
PREFACE
1 FLUORESCENCE SPECTROSCOPY
1.1 FUNDAMENTALS OF FLUORESCENCE SPECTROSCOPY
1.2 COMMON FLUORESCENCE SPECTROSCOPY TECHNIQUES
1.3 SUMMARY AND PERSPECTIVE
REFERENCES
2 NATURALLY OCCURRING AND SYNTHETIC FLUORESCENT BIOMOLECULAR BUILDING BLOCKS
2.1 Introduction
2.2 NATURALLY OCCURRING EMISSIVE BIOMOLECULAR BUILDING BLOCKS
2.3 SYNTHETIC FLUORESCENT ANALOGS OF BIOMOLECULAR BUILDING BLOCKS
2.4 SUMMARY AND PERSPECTIVE
REFERENCES
3 POLARIZED SPECTROSCOPY WITH FLUORESCENT BIOMOLECULAR BUILDING BLOCKS
3.1 TRANSITION MOMENTS
3.2 LINEAR DICHROISM
3.3 MAGNETIC CIRCULAR DICHROISM
3.4 FÖRSTER RESONANCE ENERGY TRANSFER (FRET)
3.5 FLUORESCENCE ANISOTROPY
3.6 FLUORESCENT NUCLEOBASES
3.7 FLUORESCENT PEPTIDE CHROMOPHORES
3.8 SITE-SPECIFIC LINEAR DICHROISM (SSLD)
3.9 SINGLE-MOLECULE FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET)
3.10 SINGLE-MOLECULE FLUORESCENCE-DETECTED LINEAR DICHROISM (FLD)
REFERENCES
4 FLUORESCENT PROTEINS: THE SHOW MUST GO ON!
4.1 INTRODUCTION
4.2 HISTORICAL SURVEY
4.3 PHOTOPHYSICAL PROPERTIES
4.4 PHOTOCHEMICAL REACTIONS
4.5 ION SENSITIVITY
4.6 RELATION MICROSCOPY–SPECTROSCOPY FOR FLUORESCENT PROTEINS
4.7 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
5 DESIGN AND APPLICATION OF AUTOFLUORESCENT PROTEINS BY BIOLOGICAL INCORPORATION OF INTRINSICALLY FLUORESCENT NONCANONICAL AMINO ACIDS
5.1 INTRODUCTION
5.2 DESIGN AND SYNTHESIS OF FLUORESCENT BUILDING BLOCKS IN PROTEINS
5.3 APPLICATION OF FLUORESCENT BUILDING BLOCKS IN PROTEINS
5.4 CONCLUSIONS
5.5 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
6 FLUOROMODULES: FLUORESCENT DYE–PROTEIN COMPLEXES FOR GENETICALLY ENCODABLE LABELS
6.1 INTRODUCTION
6.2 FLUOROMODULE DEVELOPMENT AND CHARACTERIZATION
6.3 IMPLEMENTATION
6.4 CONCLUSIONS
6.5 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
7 DESIGN OF ENVIRONMENTALLY SENSITIVE FLUORESCENT NUCLEOSIDES AND THEIR APPLICATIONS
7.1 INTRODUCTION
7.2 SOLVATOCHROMIC FLUORESCENT NUCLEOSIDE ANALOGS
7.3 FLUORESCENTLY LABELED NUCLEOSIDES AND OLIGONUCLEOTIDE PROBES: COVALENT ATTACHMENT OF SOLVATOCHROMIC FLUOROPHORES ONTO THE NATURAL BASES
7.4 NUCLEOSIDES WITH DUAL FLUORESCENCE FOR MONITORING DNA HYBRIDIZATION
7.5 APPROACH FOR DEVELOPING ENVIRONMENTALLY SENSITIVE FLUORESCENT (ESF) NUCLEOSIDES
7.6 BASE-SELECTIVE FLUORESCENT ESF PROBE
7.7 MOLECULAR BEACON (MB) AND ESF NUCLEOSIDES
7.8 SUMMARY AND FUTURE OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
8 EXPANDING THE NUCLEIC ACID CHEMIST'S TOOLBOX: FLUORESCENT CYTIDINE ANALOGS
8.1 INTRODUCTION
8.2 DESIGN AND CHARACTERIZATION OF FLUORESCENT C ANALOGS
8.3 IMPLEMENTATION
8.4 CONCLUSIONS
8.5 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
9 SYNTHESIS AND FLUORESCENCE PROPERTIES OF NUCLEOSIDES WITH PYRIMIDOPYRIMIDINE-TYPE BASE MOIETIES
9.1 INTRODUCTION
9.2 DISCOVERY, DESIGN, AND SYNTHESIS OF PYRIMIDOPYRIMIDINE NUCLEOSIDES
9.3 IMPLEMENTATION
9.4 CONCLUSIONS
9.5 PROSPECTS AND OUTLOOK
REFERENCES
10 FÖRSTER RESONANCE ENERGY TRANSFER (FRET) BETWEEN NUCLEOBASE ANALOGS – A TOOL FOR DETAILED STRUCTURE AND DYNAMICS INVESTIGATIONS
10.1 INTRODUCTION
10.2 THE TRICYCLIC CYTOSINE FAMILY
10.3 DEVELOPMENT OF THE FIRST NUCLEIC ACID BASE ANALOG FRET PAIR
10.4 CONCLUSIONS
10.5 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
11 FLUORESCENT PURINE ANALOGS THAT SHED LIGHT ON DNA STRUCTURE AND FUNCTION
11.1 INTRODUCTION
11.2 DESIGN, PHOTOPHYSICAL PROPERTIES, AND APPLICATIONS OF PURINE MIMICS
11.3 IMPLEMENTATION
11.4 CONCLUSIONS
11.5 PROSPECTS AND OUTLOOK
REFERENCES
12 DESIGN AND PHOTOPHYSICS OF ENVIRONMENTALLY SENSITIVE ISOMORPHIC FLUORESCENT NUCLEOSIDES
12.1 INTRODUCTION
12.2 DESIGNING ENVIRONMENTALLY SENSITIVE EMISSIVE NUCLEOSIDES
12.3 TWO ISOMORPHIC ENVIRONMENTALLY SENSITIVE DESIGNS
12.4 PROBING ENVIRONMENTAL SENSITIVITY
12.5 RECENT ADVANCEMENTS IN ISOMORPHIC FLUORESCENT NUCLEOSIDE ANALOGS
12.6 SUMMARY
12.7 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
13 SITE-SPECIFIC FLUORESCENT LABELING OF NUCLEIC ACIDS BY GENETIC ALPHABET EXPANSION USING UNNATURAL BASE PAIR SYSTEMS
13.1 INTRODUCTION
13.2 DEVELOPMENT OF UNNATURAL BASE PAIR SYSTEMS AND THEIR APPLICATIONS
13.3 IMPLEMENTATION
13.4 CONCLUSIONS
13.5 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
14 FLUORESCENT C-NUCLEOSIDES AND THEIR OLIGOMERIC ASSEMBLIES
14.1 INTRODUCTION
14.2 DESIGN, SYNTHESIS, CHARACTERIZATION, AND PROPERTIES OF FLUORESCENT C-GLYCOSIDE MONOMERS
14.3 IMPLEMENTATION OF FLUORESCENT C-GLYCOSIDE MONOMERS
14.4 OLIGOMERS OF FLUORESCENT C-GLYCOSIDES: DESIGN, SYNTHESIS, AND PROPERTIES
14.5 IMPLEMENTATION OF FLUORESCENT C-GLYCOSIDE OLIGOMERS
14.6 CONCLUSIONS
14.7 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
15 MEMBRANE FLUORESCENT PROBES: INSIGHTS AND PERSPECTIVES
ABBREVIATIONS
15.1 INTRODUCTION
15.2 NBD-LABELED LIPIDS: MONITORING SLOW SOLVENT RELAXATION IN MEMBRANES
15.3 -AS MEMBRANE PROBES: DEPTH-DEPENDENT SOLVENT RELAXATION AS MEMBRANE DIPSTICK
15.4 PYRENE: A MULTIPARAMETER MEMBRANE PROBE
15.5 CONCLUSION AND FUTURE PERSPECTIVES
ACKNOWLEDGMENTS
REFERENCES
16 LIPOPHILIC FLUORESCENT PROBES: GUIDES TO THE COMPLEXITY OF LIPID MEMBRANES
16.1 INTRODUCTION
16.2 LIPIDS, LIPID BILAYERS, AND BIOMEMBRANES
16.3 LIPID PHASES, PHASE SEPARATION, AND LIPID ORDERING
16.4 FLUORESCENT PROBES FOR MEMBRANE STUDIES
16.5 CONCLUSIONS
16.6 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
17 FLUORESCENT NEUROTRANSMITTER ANALOGS
17.1 INTRODUCTION
17.2 DESIGN AND OPTICAL PROPERTIES OF FLUORESCENT NEUROTRANSMITTERS
17.3 APPLICATIONS OF FLUORESCENT NEUROTRANSMITTERS
17.4 CONCLUSIONS
17.5 PROSPECTS AND OUTLOOK
ACKNOWLEDGMENTS
REFERENCES
INDEX
END USER LICENSE AGREEMENT
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Guide
Cover
Table of Contents
Preface
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