Details

<p>Foreword <i>Michele Calos</i> vii</p> <p>Preface ix</p> <p>Contributors xi</p> <p><b>Part I: Cloning and Gene Delivery 1</b></p> <p>1. DNA Assembly Technologies Based on Homologous Recombination 3<br /> <i>Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen</i></p> <p>2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells 19<br /> <i>Takefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan D. Chesnut</i></p> <p>3. Strategies for the Delivery of Naked DNA 37<br /> <i>Jennifer C. Moore and Ronald P. Hart</i></p> <p><b>Part II: Nonintegrating Technologies 49</b></p> <p>4. Episomal Vectors 51<br /> <i>Junying Yu</i></p> <p>5. Nonintegrating DNA Virus 85<br /> <i>Chris Kemp and David Kuninger</i></p> <p>6. Nonintegrating RNA Viruses 103<br /> <i>Noemi Fusaki</i></p> <p>7. Protein Delivery 119<br /> <i>Xiao-Jian Han and Kazuhito Tomizawa</i></p> <p><b>Part III: Integrating Technologies 135</b></p> <p>8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery 137<br /> <i>Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and R. Scott McIvor</i></p> <p>9. Integrating Viral Vectors for Gene Modifications 163<br /> <i>Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek</i></p> <p>10. Bacteriophage Integrases for Site-Specific Integration 199<br /> <i>Bhaskar Thyagarajan</i></p> <p>11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211<br /> <i>Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu</i></p> <p><b>Part IV: Applications 227</b></p> <p>12. Modified Stem Cells as Disease Models and in Toxicology Screening 229<br /> <i>Catharina Ellerstr€om, Peter Sartipy, Raimund Strehl, and Johan Hyllner</i></p> <p>13. Screening and Drug Discovery 251<br /> <i>Elizabeth A. Davenport and Robert S. Ames</i></p> <p>Index 273</p>

<p>“This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells.  Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy.”  (<i>Doody’s</i>, 17 August 2012)</p> <p> </p> <p> </p> <p> </p>

<b>UMA LAKSHMIPATHY</b> is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications. <p><b>BHASKAR THYAGARAJAN</b> is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.</p>

<b>The all-important resource that lays the groundwork for researchers to genetically modify primary and stem cells for use in downstream applications</b> <p>Replacing damaged or lost organs by producing "spare parts" is a concept that lies at the core of the varied biotechnological practices generally referred to as tissue engineering. An important component in this regeneration process includes the use of stem cells, which harbor the ability to mature into other cell types and hold the potential to act as a repair system for the body. <i>Primary and Stem Cells</i> describes basic cell engineering methods, emphasizing stem cell applications, and the use of genetically modified stem cells in cell therapy and drug discovery. Discussion includes new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems, along with:</p> <ul> <li> <p>Transfection techniques and methods for cell line engineering</p> </li> <li> <p>Information that assists researchers and drug developers in deciding how to generate their required drug or gene therapists in developing genetically modified cells</p> </li> <li> <p>An overview of applications in areas like disease models and drug discovery</p> </li> <li> <p>Issues that address the interests of companies involved in stem cell tools and reagents</p> </li> </ul> <p><i>Primary and Stem Cells</i> serves as an essential guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose —basic science investigation, creation of disease models and screens, or cells for therapeutic applications—and acts as the launching pad for pioneering further development in a field emerging with exciting possibilities.</p>

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