Details

Measurement of Antioxidant Activity and Capacity


Measurement of Antioxidant Activity and Capacity

Recent Trends and Applications
Hui: Food Science and Technology 1. Aufl.

von: Resat Apak, Esra Capanoglu, Fereidoon Shahidi

162,99 €

Verlag: Wiley
Format: PDF
Veröffentl.: 30.11.2017
ISBN/EAN: 9781119135364
Sprache: englisch
Anzahl Seiten: 352

DRM-geschütztes eBook, Sie benötigen z.B. Adobe Digital Editions und eine Adobe ID zum Lesen.

Beschreibungen

A comprehensive reference for assessing the antioxidant potential of foods and essential techniques for developing healthy food products Measurement of Antioxidant Activity and Capacity offers a much-needed resource for assessing the antioxidant potential of food and includes proven approaches for creating healthy food products. With contributions from world-class experts in the field, the text presents the general mechanisms underlying the various assessments, the types of molecules detected, and the key advantages and disadvantages of each method. Both thermodynamic (i.e. efficiency of scavenging reactive species) and kinetic (i.e. rates of hydrogen atom or electron transfer reactions) aspects of available methods are discussed in detail.  A thorough description of all available methods provides a basis and rationale for developing standardized antioxidant capacity/activity methods for food and nutraceutical sciences and industries. This text also contains data on new antioxidant measurement techniques including nanotechnological methods in spectroscopy and electrochemistry, as well as on innovative assays combining several principles. Therefore, the comparison of conventional methods versus novel approaches is made possible. This important resource: Offers suggestions for assessing the antioxidant potential of foods and their components Includes strategies for the development of healthy functional food products Contains information for identifying antioxidant activity in the body Presents the pros and cons of the available antioxidant determination methods, and helps in the selection of the most appropriate method Written for researchers and professionals in the nutraceutical and functional food industries,academia and government laboratories, this text includes the most current knowledge in order to form a common language between research groups and to contribute to the solution of critical problems existing for all researchers working in this field. 
List of contributors xi 1 Nomenclature and general classification of antioxidant activity/capacity assays 1Yong Sun, Cheng Yang, and Rong Tsao 1.1 Introduction 1 1.2 Nomenclature of antioxidant activity/capacity assays 2 1.3 Classification of antioxidant activity/capacity assays 2 1.4 Conclusions 15 References 15 2 Assays based on competitive measurement of the scavenging ability of reactive oxygen/nitrogen species 21Dejian Huang and Restituto Tocmo 2.1 Introduction 21 2.2 Kinetics is more important than thermodynamics when it comes to scavenging ROS 22 2.3 Peroxyl radical scavenging capacity assay based on inhibition of lipid autoxidation 23 2.4 Application of molecular probes for quantification of antioxidant capacity in scavenging specific ROS/RNS 26 2.5 Conclusion: a unified approach for measuring antioxidant capacity against different ROS? 35 Acknowledgment 36 References 36 3 Evaluation of the antioxidant capacity of food samples: a chemical examination of the oxygen radical absorbance capacity assay 39Eva Dorta, Eduardo Fuentes?]Lemus, Hernan Speisky, Eduardo Lissi, and Camilo Lopez?]Alarcon 3.1 Introduction 39 3.2 Chemical assays to evaluate the antioxidant capacity of food samples 41 3.3 Chemical examination of the ORAC assay: advantages and drawbacks 46 3.4 Future perspectives to improve the antioxidant capacity evaluation of food samples 50 3.5 Conclusions 52 Acknowledgments 52 References 52 4 Electron transfer?]based antioxidant capacity assays and the cupricion reducing antioxidant capacity (CUPRAC) assay 57Resat Apak 4.1 Introduction 57 4.2 ET?]based TAC assays 58 4.3 CUPRAC assay of antioxidant capacity measurement 64 References 71 5 The ferric reducing/antioxidant power (FRAP) assay for non?]enzymatic antioxidant capacity: concepts, procedures, limitations and applications 77Iris F.F. Benzie and Malegaddi Devaki 5.1 Introduction: concepts and context 77 5.2 The ferric reducing/antioxidant power (FRAP) assay: a brief overview 79 5.3 Working concepts, what results represent, potential interferences, and limitations 80 5.4 Method outline and detailed procedures for manual, semi?]automated, and fully automated modes 83 5.5 Technical tips for the FRAP assay 89 5.6 Issues of standardization (calibration) and how results are expressed 93 5.7 Issues of sample handling, storage, and extraction 94 5.8 Modifications to the FRAP assay 94 5.9 Illustrative applications 99 5.10 Cautions and concluding remarks 99 Acknowledgments 102 References 102 Further Reading 104 6 Folin–Ciocalteu method for the measurement of total phenolic content and antioxidant capacity 107Rosa M. Lamuela?]Raventos 6.1 Introduction 107 6.2 Is the Folin–Ciocalteu method an antioxidant assay? 107 6.3 Folin–Ciocalteu assay to quantify phenolic compounds 108 6.4 Folin–Ciocalteu index in wines 109 6.5 Improving the method: more sustainability, less time, and lower cost 110 6.6 Beneficial effects of polyphenols measured by the Folin–Ciocalteu assay in human biological samples: a biomarker of polyphenol intake 114 References 114 7 ABTS/TEAC (2,2??]azino?]bis(3?]ethylbenzothiazoline?]6?]sulfonic acid)/ TroloxR?]Equivalent Antioxidant Capacity) radical scavenging mixed?]mode assay 117Antonio Cano and Marino B. Arnao 7.1 Introduction 117 7.2 Use of ABTS as a sensor of antioxidant activity: the TEAC assay 119 7.3 Advantages and disadvantages 125 7.4 TEAC assay in hyphenated and high?]throughput techniques 126 7.5 TEAC in pure compounds 128 7.6 TEAC in foods 130 7.7 Future perspectives 134 References 135 8 DPPH (2,2?]di(4?]tert?]octylphenyl)?]1?]picrylhydrazyl) radical scavenging mixed?]mode colorimetric assay(s) 141Nikolaos Nenadis and Maria Z. Tsimidou 8.1 Overview 141 8.2 Characteristics of the DPPH radical 142 8.3 The concept behind the development of the DPPH• colorimetric assay 144 8.4 How can antioxidants scavenge the DPPH•? 144 8.5 The evolution of ideas on the underlying mechanism 145 8.6 The DPPH• colorimetric assay(s) 152 8.7 Toward the standardization of a DPPH• assay to address structure–activity relationship issues 154 8.8 Toward the establishment of a DPPH• assay for regulatory and market needs 158 8.9 Concluding remarks – A la recherche du temps perdu 160 References 161 9 Biomarkers of oxidative stress and cellular?]based assays of indirect antioxidant measurement 165Cheng Yang, Fereidoon Shahidi, and Rong Tsao 9.1 Introduction 165 9.2 Oxidative stress 166 9.3 Biomarkers of oxidative stress 169 9.4 Cell?]based assays of indirect antioxidant measurement 175 9.5 Conclusion 180 References 181 10 Nanotechnology?]enabled approaches for the detection of antioxidants by spectroscopic and electrochemical methods 187Ryan T. Rauhut, Gonca Bulbul, and Silvana Andreescu 10.1 Introduction 187 10.2 Spectroscopic nano?]based approaches for antioxidant detection 190 10.3 Electrochemical detection 195 10.4 Conclusions and future research needs 200 Acknowledgments 200 References 204 11 Novel methods of antioxidant assay combining various principles 209Takayuki Shibamoto 11.1 Introduction 209 11.2 Lipid peroxidation and formation of primary and secondary oxidation products 210 11.3 Use of gas chromatography for antioxidant assays 211 11.4 Novel gas chromatographic antioxidant assays 213 11.5 Conclusion 218 References 218 12 Physico?]chemical principles of antioxidant action, including solvent and matrix dependence and interfacial phenomena 225Katarzyna Jodko?]Piorecka, Jakub Cedrowski, and Grzegorz Litwinienko 12.1 Introduction 225 12.2 Mechanism and kinetics of peroxidation 226 12.3 Initiation of lipid peroxidation chains 227 12.4 Antioxidants 232 12.5 How to recognize a good chain?]breaking antioxidant 234 12.6 Determination of reactivity of a CBA towards peroxyl radicals 236 12.7 Basic mechanisms of antioxidant action 247 12.8 Interfacial phenomena – studies in heterogeneous lipid systems 252 12.9 Effect of temperature 265 Acknowledgments 267 References 267 13 Evaluation of antioxidant activity/capacity measurement methods for food products 273Esra Capanoglu, Senem Kamiloglu, Gulay Ozkan, and Resat Apak 13.1 Introduction 273 13.2 Antioxidant assay selection for different food products 276 13.3 General conclusions and future perspectives 281 References 283 14 Antioxidants in oxidation control 287Fereidoon Shahidi and Priyatharini Ambigaipalan 14.1 Introduction 287 14.2 Oxidation 287 14.3 Antioxidants 288 14.4 Synthetic antioxidants 289 14.5 Natural antioxidants 289 14.6 Tocols 290 14.7 Ascorbic acid 291 14.8 Carotenoids 292 14.9 Polyphenols 295 14.10 Bioavailability of phenolic antioxidants 307 14.11 Structural and other modification of phenolic antioxidants 308 14.12 Protein?]derived antioxidants 309 14.13 Phospholipids 309 14.14 Other antioxidants 310 References 310 15 Kinetic matching approach for rapid assessment of endpoint antioxidant capacity 321Luis M. Magalhaes, Ines I. Ramos, Luisa Barreiros, Salette Reis, and Marcela A. Segundo 15.1 Introduction 321 15.2 Kinetic matching strategy 323 15.3 Expression of results as common standard 323 15.4 Application to samples 324 15.5 Conclusion 329 Acknowledgments 329 References 330 Index 333
About the editors Resat Apak is Professor of Analytical Chemistry Division, Department of Chemistry, Faculty of Engineering, Istanbul University, Turkey. Esra Capanoglu is Associate Professor at the Food Engineering Department, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Turkey. Fereidoon Shahidi is a University Research Professor at the Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
A comprehensive reference for assessing the antioxidant potential of foods and essential techniques for developing healthy food products Measurement of Antioxidant Activity & Capacity offers a much-needed resource for assessing the antioxidant potential of food and includes proven approaches for creating healthy food products. With contributions from world-class experts in the field, the text presents the general mechanisms underlying the various assessments, the types of molecules detected, and the key advantages and disadvantages of each method. Both thermodynamic (i.e. efficiency of scavenging reactive species) and kinetic (i.e. rates of hydrogen atom or electron transfer reactions) aspects of available methods are discussed in detail. A thorough description of all available methods provides a basis and rationale for developing standardized antioxidant capacity/activity methods for food and nutraceutical sciences and industries. This text also contains data on new antioxidant measurement techniques including nanotechnological methods in spectroscopy and electrochemistry, as well as on innovative assays combining several principles. Therefore, the comparison of conventional methods versus novel approaches is made possible. This important resource: Offers suggestions for assessing the antioxidant potential of foods and their components Includes strategies for the development of healthy functional food products Contains information for identifying antioxidant activity in the body Presents the pros and cons of the available antioxidant determination methods, and helps in the selection of the most appropriate method Written for researchers and professionals in the nutraceutical and functional food industries, academia and government laboratories, this text includes the most current knowledge in order to form a common language between research groups and to contribute to the solution of critical problems existing for all researchers working in this field.

Diese Produkte könnten Sie auch interessieren:

Microstructure of Dairy Products
Microstructure of Dairy Products
von: Mamdouh El-Bakry, Antoni Sanchez, Bhavbhuti M. Mehta
PDF ebook
151,99 €
Microstructure of Dairy Products
Microstructure of Dairy Products
von: Mamdouh El-Bakry, Antoni Sanchez, Bhavbhuti M. Mehta
EPUB ebook
151,99 €
Handbook of Vanilla Science and Technology
Handbook of Vanilla Science and Technology
von: Daphna Havkin-Frenkel, Faith C. Belanger
PDF ebook
152,99 €