Details

Extracellular Targeting of Cell Signaling in Cancer


Extracellular Targeting of Cell Signaling in Cancer

Strategies Directed at MET and RON Receptor Tyrosine Kinase Pathways
1. Aufl.

von: James W. Janetka, Roseann M. Benson

156,99 €

Verlag: Wiley
Format: PDF
Veröffentl.: 10.05.2018
ISBN/EAN: 9781119300205
Sprache: englisch
Anzahl Seiten: 488

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Beschreibungen

<p><b>International experts present innovative therapeutic strategies to treat cancer patients and prevent disease progression</b></p> <p><i>Extracellular Targeting of Cell Signaling in Cancer</i> highlights innovative therapeutic strategies to treat cancer metastasis and prevent tumor progression. Currently, there are no drugs available to treat or prevent metastatic cancer other than non-selective, toxic chemotherapy.  With contributions from an international panel of experts in the field, the book integrates diverse aspects of biochemistry, molecular biology, protein engineering, proteomics, cell biology, pharmacology, biophysics, structural biology, medicinal chemistry and drug development.</p> <p>A large class of proteins called kinases are enzymes required by cancer cells to grow, proliferate, and survive apoptosis (death) by the immune system. Two important kinases are MET and RON which are receptor tyrosine kinases (RTKs) that initiate cell signaling pathways outside the cell surface in response to extracellular ligands (growth factors.) Both kinases are oncogenes which are required by cancer cells to migrate away from the primary tumor, invade surrounding tissue and metastasize. MET and RON reside on both cancer cells and the support cells surrounding the tumor, called the microenvironment. MET and RON are activated by their particular ligands, the growth factors HGF and MSP, respectively. Blocking MET and RON kinase activation and downstream signaling is a promising therapeutic strategy for preventing tumor progression and metastasis. Written for cancer physicians and biologists as well as drug discovery and development teams in both industry and academia, this is the first book of its kind which explores novel approaches to inhibit MET and RON kinases other than traditional small molecule kinase inhibitors. These new strategies target key tumorigenic processes on the outside of the cell, such as growth factor activation by proteases. These unique strategies have promising potential as an improved alternative to kinase inhibitors, chemotherapy, or radiation treatment. </p>
<p>List of Contributors xiii</p> <p>Preface xvii</p> <p><b>1 Discovery and Function of the HGF/MET and the MSP/RON Kinase Signaling Pathways in Cancer 1<br /></b><i>Silvia Benvenuti, Melissa Milan and Paolo M. Comoglio</i></p> <p>1.1 Introduction 1</p> <p>1.2 MET Tyrosine Kinase Receptor and its Ligand HGF: Structure 1</p> <p>1.2.1 The Invasive growth Program 2</p> <p>1.2.2 MET Mediated Signaling 4</p> <p>1.2.2.1 MET Down-regulation 7</p> <p>1.2.3 Cross-talk between MET and Other Receptors 7</p> <p>1.2.4 MET Activation in Human Cancers 9</p> <p>1.2.4.1 MET, Hypoxia and Ionizing Radiations 10</p> <p>1.2.4.2 MET Expression in Cancer Stem Cells: a Paradigm of Inherence 11</p> <p>1.2.4.3 Oncogene Addiction and Oncogene Expedience 11</p> <p>1.2.5 Targeting HGF/MET as a Therapeutic Approach in Human Cancer 12</p> <p>1.2.5.1 HGF Antagonists 13</p> <p>1.2.5.2 Tyrosine Kinase Inhibitors 15</p> <p>1.2.5.3 Anti-MET Monoclonal Antibodies 17</p> <p>1.2.5.4 Alternative MET Blocking Strategies 18</p> <p>1.2.6 Primary and Secondary Resistance 18</p> <p>1.2.6.1 MET Role in Resistance to Anticancer Agents 19</p> <p>1.2.6.2 Mechanism of Resistance to MET Inhibitors 19</p> <p>1.2.6.3 Combinatorial Therapeutic Strategies 20</p> <p>1.3 RON Tyrosine Kinase Receptor and its Ligand MSP 21</p> <p>1.3.1 Discovery and Structural Biology 21</p> <p>1.3.2 RON Mediated Signaling 25</p> <p>1.3.3 Cross-talk between RON and other Receptors 26</p> <p>1.3.4 RON Activation in Human Cancers 26</p> <p>1.4 Targeting MSP/RON as a Therapeutic Approach in Human Cancer 27</p> <p>1.5 Concluding Remarks 28</p> <p><b>2 The Role of HGF/MET and MSP/RON Signaling in Tumor Progression and Resistance to Anticancer Therapy 45<br /></b><i>Lidija Klampfer and Benjamin Yaw Owusu</i></p> <p>2.1 Introduction 45</p> <p>2.2 HGF/MET Signaling in Cancer 47</p> <p>2.3 MSP/RON Signaling in Cancer 52</p> <p>2.4 Cross-talk between MET and RON Signaling Pathways 53</p> <p>2.5 HGF/MET and MSP/RON Signaling Elicit Resistance to Cancer Therapy 55</p> <p>2.6 Conclusions and Perspectives 58</p> <p>References 58</p> <p><b>3 HGF Activator (HGFA) and its Inhibitors HAI-1 and HAI-2: Key Players in Tissue Repair and Cancer 69<br /></b><i>Hiroaki Kataoka and Takeshi Shimomura</i></p> <p>3.1 Introduction 69</p> <p>3.2 Discovery of HGFA 70</p> <p>3.2.1 Tissue Injury-induced Activation of HGF 70</p> <p>3.2.2 Identification of HGFA as a Serum Activator of pro-HGF 71</p> <p>3.3 Synthesis of HGFA Zymogen in vivo 71</p> <p>3.4 Molecular Structure of HGFA 72</p> <p>3.4.1 The Gene Encoding pro-HGFA: HGFAC 72</p> <p>3.4.2 ProHGFA Protein and its Activation 72</p> <p>3.4.3 Structure Biology of HGFA 74</p> <p>3.5 Substrates of HGFA in vivo 75</p> <p>3.6 Regulation of HGFA Activity by Endogenous Inhibitors 76</p> <p>3.6.1 HGF Activator Inhibitor-1 (HAI-1): a Cell Surface Regulator of HGFA Activity 76</p> <p>3.6.2 HGF Activator Inhibitor-2 (HAI-2) 78</p> <p>3.6.3 Protein C Inhibitor (PCI; SERPINA5) 78</p> <p>3.7 Proposed Biological Functions of HGFA in vivo 78</p> <p>3.8 Roles of HGFA in Cancer 80</p> <p>3.8.1 Enhanced Activation of pro-HGF and pro-MSP in Cancer Tissues 80</p> <p>3.8.2 Possible Roles of HGFA in Cancer Progression 80</p> <p>3.9 Conclusions and Future Perspectives of HGFA Research in Cancer 82</p> <p>References 83</p> <p><b>4 Physiological Functions and Role of Matriptase in Cancer 91<br /></b><i>Fausto A. Varela, Thomas E. Hyland and Karin List</i></p> <p>4.1 Introduction 91</p> <p>4.2 Discovery of Matriptase 91</p> <p>4.3 Biochemical and Functional Characteristics of Matriptase – Inhibitors, Substrates and Structure 92</p> <p>4.3.1 Endogenous Polypeptide Matriptase Inhibitors 92</p> <p>4.3.2 Matriptase Substrates 94</p> <p>4.3.3 Matriptase Structure 95</p> <p>4.4 Physiological and Pathophysiological Functions of Matriptase 96</p> <p>4.4.1 Matriptase in Epidermal Development and Homeostasis 96</p> <p>4.4.2 Matriptase in the Gastrointestinal Tract 97</p> <p>4.4.3 Matriptase in Thymocytes and Salivary Glands 98</p> <p>4.4.4 Matriptase in Placental/Embryonic Development 98</p> <p>4.4.5 Matriptase in Neural Tube Closure 99</p> <p>4.4.6 Pathways requiring Matriptase 99</p> <p>4.4.7 Matriptase in Viral Infection 101</p> <p>4.5 Role of Matriptase in Cancer 101</p> <p>4.5.1 Studying Matriptase in Cultured Cancer Cells and Tumor Grafting Models 108</p> <p>4.5.2 In vivo Cancer Studies using Genetic Models 111</p> <p>4.5.2.1 Squamous Cell Carcinoma 111</p> <p>4.5.2.2 Colitis-associated Colon Carcinogenesis 112</p> <p>4.5.2.3 Breast Cancer 112</p> <p>4.6 Conclusions 114</p> <p>References 114</p> <p><b>5 The Cell-Surface, Transmembrane Serine Protease Hepsin: Discovery, Function and Role in Cancer 125<br /></b><i>Denis Belitškin, Shishir Mani Pant, Topi Tervonen and Juha Klefström</i></p> <p>5.1 Biology of Hepsin 125</p> <p>5.1.1 Discovery of Hepsin 125</p> <p>5.1.1.1 Cloning of Hepsin, HPN Gene 125</p> <p>5.1.1.2 Assigning Hepsin to Type II Transmembrane Serine Protease Family 126</p> <p>5.1.2 Hepsin Gene and Protein 126</p> <p>5.1.2.1 Expression, Regulation and Structure 126</p> <p>5.1.2.2 Hepsin Activation and Activity 130</p> <p>5.1.3 Physiological Functions of Hepsin 131</p> <p>5.1.3.1 Growth Factor Activation 131</p> <p>5.1.3.2 Serine Protease Cascades 132</p> <p>5.1.3.3 Cell Proliferation and Motility 132</p> <p>5.1.3.4 Epithelial Integrity 133</p> <p>5.1.3.5 Organ Development 135</p> <p>5.2 Hepsin in Cancer 137</p> <p>5.2.1 Gain of Oncogenic Function 137</p> <p>5.2.1.1 Genetic Alterations 137</p> <p>5.2.1.2 Altered Subcellular Localization 138</p> <p>5.2.1.3 Oncogenic Hepsin Function in vivo 140</p> <p>5.2.1.4 How HPN Promotes Cancer 141</p> <p>5.2.2 Targeting Hepsin in Cancer 143</p> <p>5.3 Future Prospects 144</p> <p>5.3.1 Hepsin’s Role as Guardian of Epithelial Integrity 144</p> <p>5.3.2 Cancer Disease Progression and Metastasis 145</p> <p>5.3.2.1 Uncontrolled Proteolysis 145</p> <p><b>6 Targeting HGF with Antibodies as an Anti-Cancer Therapeutic Strategy 155<br /></b><i>Dinuka M. De Silva, Arpita Roy and Donald P. Bottaro</i></p> <p>6.1 Introduction 155</p> <p>6.2 HGF Biology 156</p> <p>6.2.1 HGF Gene Organization and mRNA Transcripts 156</p> <p>6.2.2 HGF Protein Isoforms and Proteolytic Processing 156</p> <p>6.2.2.1 HGF Isoforms 156</p> <p>6.2.2.2 HGF Activation by Proteolytic Processing 159</p> <p>6.2.3 Key HGF Interactions: Heparan Sulfate Proteoglycans and Met 160</p> <p>6.2.3.1 Heparan Sulfate Proteoglycans 160</p> <p>6.2.3.2 Met and Key Intracellular Effectors 161</p> <p>6.2.4 Major Sites of HGF Expression: Tissues and Organs 162</p> <p>6.2.5 HGF Function in Development and Adulthood 162</p> <p>6.2.5.1 hgf or met altered Mice: Embryogenesis 163</p> <p>6.2.5.2 hgf or met altered Mice: Late Development and Adulthood 163</p> <p>6.3 HGF in Cancer 164</p> <p>6.3.1 Lung Cancer 165</p> <p>6.3.2 Hepatocellular Carcinoma 165</p> <p>6.3.3 Genitourinary Malignancies 166</p> <p>6.3.4 Breast Cancer 167</p> <p>6.3.5 Colorectal and Gastric Carcinomas 167</p> <p>6.3.6 Papillary Thyroid Carcinoma 168</p> <p>6.3.7 Brain Tumors 168</p> <p>6.3.8 Melanoma 169</p> <p>6.3.9 Head and Neck Squamous Cell Carcinoma 169</p> <p>6.3.10 Other Malignancies 169</p> <p>6.4 Anti-HGF Monoclonal Antibodies as Anti-Cancer Therapeutic</p> <p>Candidates 170</p> <p>6.4.1 Rilotumumab 170</p> <p>6.4.2 Ficlatuzumab 174</p> <p>6.4.3 TAK-701 175</p> <p>6.5 Conclusions and Future Directions 176</p> <p>Acknowledgements 177</p> <p>References 177</p> <p><b>7 MET and RON Receptor Tyrosine Kinases as Therapeutic Antibody Targets for Cancer 199<br /></b><i>Mark Wortinger, Jonathan Tetreault, Nick Loizos, and Ling Liu</i></p> <p>7.1 MET as a Therapeutic Antibody Target for Cancer 199</p> <p>7.2 Challenges in Developing MET Therapeutic Antibodies 200</p> <p>7.3 Anti-MET Antibody Clinical Diagnostics 203</p> <p>7.4 Anti-MET Antibodies in the Clinic 204</p> <p>7.4.1 Onartuzumab – Roche 204</p> <p>7.4.2 Emibetuzumab – Eli Lilly 206</p> <p>7.4.3 ABT-700 – AbbVie 208</p> <p>7.4.4 SAIT301 – Samsung 208</p> <p>7.4.5 ARGX-111 – Argenx 209</p> <p>7.4.6 Sym-015 – Symphogen 210</p> <p>7.5 Additional anti-MET Antibodies 210</p> <p>7.5.1 DN-30 – University of Turin Medical School 210</p> <p>7.5.2 Other Preclinical Stage anti-MET Antibodies 210</p> <p>7.6 Summary– anti-MET Antibodies 211</p> <p>7.7 RON as a Therapeutic Antibody Target for Cancer 211</p> <p>7.8 Conclusions and Future Outlook 216</p> <p>References 216</p> <p><b>8 Inhibitory Antibodies of the Proteases HGFA, Matriptase and Hepsin  229<br /></b><i>Daniel Kirchhofer, Charles Eigenbrot, and Robert A. Lazarus</i></p> <p>8.1 Anti-Serine Protease Antibodies for Therapeutic Applications 229</p> <p>8.2 Antibodies can Inhibit Trypsin-Fold Serine Proteases in Diverse Ways 230</p> <p>8.2.1 Orthosteric Inhibition (Active Site Binding) 231</p> <p>8.2.2 Allosteric Inhibition 231</p> <p>8.2.3 Exosite Inhibition 231</p> <p>8.2.4 Inhibition of Zymogen Activation 231</p> <p>8.2.5 Cofactor Inhibition 231</p> <p>8.2.6 Inactivation of Oligomeric Serine Proteases 232</p> <p>8.2.7 Comparison of Abs with Natural Occurring Protein Modes of Inhibition 232</p> <p>8.3 Introduction to Antibodies against HGFA, Matriptase and Hepsin 233</p> <p>8.4 Inhibitory HGFA Antibodies 234</p> <p>8.5 Inhibitory Matriptase Antibodies 238</p> <p>8.6 Inhibitory Hepsin Antibodies 239</p> <p>8.7 Conclusion 240</p> <p>References 240</p> <p><b>9 Inhibitors of the Growth-Factor Activating Proteases Matriptase, Hepsin and HGFA: Strategies for Rational Drug Design and Optimization 247<br /></b><i>James W. Janetka and Robert A. Galemmo, Jr</i></p> <p>9.1 Introduction 247</p> <p>9.1.1 Proteolytic Control of HGF/MET Oncogenic Signaling 247</p> <p>9.1.2 Proteolytic Control of MSP/RON Kinase Signaling 248</p> <p>9.1.3 The Identification of HGF and MSP Converting Enzyme Activity 249</p> <p>9.2 Small Molecular Weight Inhibitors of HGFA, Matriptase and Hepsin 251</p> <p>9.2.1 Mechanism-based Inhibitors derived from Substrate Sequences 251</p> <p>9.2.2 Approved Drugs as Starting Points for Inhibitor Design 257</p> <p>9.2.3 Retro-Engineering Inhibitors of Related Proteases 258</p> <p>9.3 Improving Drug-like Properties of the Current Inhibitors: Lessons from the Oral Anti-Coagulants 264</p> <p>9.4 Conclusion 269</p> <p>References 270</p> <p><b>10 Cyclic Peptide Serine Protease Inhibitors Based on the Natural Product SFTI-1 277<br /></b><i>Blake T. Riley, Olga Ilyichova, Jonathan M. Harris, David E. Hoke and Ashley M. Buckle</i></p> <p>10.1 Introduction: Naturally Occurring Polypeptide Serine Protease Inhibitors 277</p> <p>10.1.1 Serpins 277</p> <p>10.1.2 Standard Mechanism Inhibitors 278</p> <p>10.1.2.1 Kunitz Type 278</p> <p>10.1.2.2 Kazal Type 278</p> <p>10.1.2.3 Bowman–Birk Inhibitor (BBI) Family 278</p> <p>10.2 Selective Inhibitors of Serine Proteases using the Sunflower Trypsin Inhibitor (SFTI-1) as a Scaffold for Rational Drug Design 279</p> <p>10.2.1 Trypsin 279</p> <p>10.2.2 Chymotrypsin, Neutrophil Elastase and Cathepsin G 286</p> <p>10.2.3 Proteasome 286</p> <p>10.2.4 Matriptase and other Type II Transmembrane Serine Proteases (TTSPs) 286</p> <p>10.2.5 MASP-1 and MASP-2 286</p> <p>10.2.6 Other KLKs (KLK5, 7, 14) 287</p> <p>10.2.7 KLK4 287</p> <p>10.3 Normal and Pathophysiological Functions of the Human Tissue Kallikrein (KLK)-related Serine Protease Family 288</p> <p>10.3.1 Physiological Role for KLKs 288</p> <p>10.3.2 KLKs and their Role in Prostate Cancer Pathogenesis 289</p> <p>10.3.3 Kallikrein-related Peptidase 4 as a Point of Therapeutic Intervention 290</p> <p>10.4 Inhibitors of KLK4 Serine Protease 291</p> <p>10.4.1 Molecular Basis of KLK4 Inhibition by SFTI-1 291</p> <p>10.4.2 Use of SFTI-1 as a Scaffold in Ligand Design and Optimization 292</p> <p>10.4.3 Identification of an Optimal Tetrapeptide Substrate 292</p> <p>10.4.4 SFTI-1FCQR is a Potent Selective Inhibitor of KLK4 293</p> <p>10.4.4.1 Structural Basis for Potency and Selectivity of SFTI-1FCQR Derivative 293</p> <p>10.5 Potential Therapeutic Applications and Challenges 294</p> <p>10.6 Conclusions/Future Directions 297</p> <p>References 297</p> <p><b>11 Screening Combinatorial Peptide Libraries in Protease Inhibitor Drug Discovery 307<br /></b><i>Marcin Poreba, Paulina Kasperkiewicz, Wioletta Rut and Marcin Drag</i></p> <p>11.1 Introduction 307</p> <p>11.2 Proteases Involved in Cancer 309</p> <p>11.2.1 Metalloproteases 309</p> <p>11.2.2 Serine Proteases 310</p> <p>11.2.3 Cysteine Proteases 311</p> <p>11.2.4 Aspartic Proteases 311</p> <p>11.2.5 Threonine Proteases 312</p> <p>11.2.6 Target Protease Substrates and Inhibitors 312</p> <p>11.3 Identification and Optimization of Preferred Substrates 313</p> <p>11.3.1 Positional Scanning of Substrate Combinatorial Libraries (PS-SCL) 313</p> <p>11.3.2 Peptide Microarrays 318</p> <p>11.3.3 Hybrid Combinatorial Substrate Library (HyCoSuL) 318</p> <p>11.3.4 Counter Selection Substrate Library (CoSeSuL) 320</p> <p>11.3.5 Combinatorial Substrate Synthesis for Aminopeptidase Screening 320</p> <p>11.3.6 Internally Quenched Fluorescent (IQF) Substrates 321</p> <p>11.3.7 Phage Display 322</p> <p>11.3.8 Protease Substrates – Summary 325</p> <p>11.4 Design of Covalent Inhibitors Based on Substrates 326</p> <p>11.4.1 Background and General Characteristics of Inhibitors 326</p> <p>11.4.2 Substrate-based Inhibitor Design and Discovery 327</p> <p>11.4.3 PS-SCL Applied to Inhibitors other than Substrates 328</p> <p>11.4.4 Inhibitors from Phage Display Screening and Directed Evolution of Proteins 331</p> <p>11.5 Anticancer Drugs – How much Information do We Need? 334</p> <p>11.6 Conclusions 336</p> <p>Acknowledgements 337</p> <p>References 337</p> <p><b>12 Chemical Probes Targeting Proteases for Imaging and Diagnostics in Cancer 351<br /></b><i>Pedro Gonçalves and Steven H. L. Verhelst</i></p> <p>12.1 Introduction 351</p> <p>12.2 Chemical Probes for Proteases 352</p> <p>12.2.1 Substrate-based Probes 352</p> <p>12.2.2 Activity-based Probes (ABPs) 356</p> <p>12.2.3 Photo-crosslinking probes 356</p> <p>12.2.4 Non-Covalent Probes 358</p> <p>12.3 Molecular Imaging of Cancer 358</p> <p>12.3.1 Imaging Tumors with Substrate-based Probes 359</p> <p>12.3.1.1 Preclinical Model Systems 359</p> <p>12.3.1.2 Clinical Trials 361</p> <p>12.3.2 Imaging Tumors with ABPs 362</p> <p>12.3.2.1 Conventional and multimodal ABPs 362</p> <p>12.3.2.2 Quenched ABPs 364</p> <p>12.3.2.3 Towards Clinical Applications 365</p> <p>12.3.3 Imaging Tumors with Affinity-based Reagents 366</p> <p>12.3.3.1 Preclinical Models 366</p> <p>12.3.3.2 Clinical Trials 367</p> <p>12.4 Conclusions 369</p> <p>Acknowledgements 370</p> <p>References 371</p> <p><b>13 Cancer Diagnostics of Protease Activity and Metastasis 377<br /></b><i>Timothy J. O’Brien and John Beard</i></p> <p>13.1 Introduction 377</p> <p>13.2 The Proteins Identified from Patient Tumor Profiling 386</p> <p>13.2.1 Matriptase 386</p> <p>13.2.2 Hepsin 387</p> <p>13.2.3 KLK7 387</p> <p>13.2.4 KLK6 388</p> <p>13.2.5 KLK8 388</p> <p>13.2.6 TMPRSS3 388</p> <p>13.2.7 MMP-7 389</p> <p>13.3 ELISA Assay Development 389</p> <p>13.4 The Role of Markers for Cancer Surveillance and Tumor Monitoring (Early Detection) 390</p> <p>13.5 Cell Signaling and the Cancer Cascade 399</p> <p>13.6 Conclusions and Future Prospects 400</p> <p>References 402</p> <p><b>14 Roles of Pericellular Proteases in Tumor Angiogenesis: Therapeutic Implications 411<br /></b><i>Janice M. Kraniak, Raymond R. Mattingly and Bonnie F. Sloane</i></p> <p>14.1 Introduction 411</p> <p>14.2 Initiation of Angiogenesis 412</p> <p>14.3 Mechanisms of New Blood Vessel Formation 413</p> <p>14.3.1 Sprouting Angiogenesis 414</p> <p>14.3.2 Intussesceptive or Non-sprouting Angiogenesis 415</p> <p>14.3.3 Neovasculogenesis 415</p> <p>14.3.4 Vascular Mimicry 416</p> <p>14.4 Pericellular Proteases and Angiogenesis 417</p> <p>14.4.1 Metalloproteinases: MMPs, ADAMs and ADAM-TS 418</p> <p>14.4.1.1 MMPs 418</p> <p>14.4.1.2 ADAMs and ADAM-TS 422</p> <p>14.4.2 Serine Proteases 424</p> <p>14.4.3 Cysteine Cathepsins 425</p> <p>14.4.3.1 Cysteine Cathepsins in Angiogenesis 426</p> <p>14.5 Novel Approaches for Targeting Tumor Angiogenesis 428</p> <p>14.6 Summary 432</p> <p>Acknowledgements 433</p> <p>References 433</p> <p>Index 447</p>
<p><b>Dr. James W. Janetka</b> is an Associate Professor at Washington University School of Medicine, and has over 20 years of medicinal chemistry and drug discovery experience within both industry and academia.  He has published 50 peer-reviewed manuscripts and holds 20 US patents in oncology and infectious disease.</p> <p><b>Roseann Benson</b> is a chemical engineer turned scientific writer and editor.  As a consultant for Harvard and Washington University Medical Schools, she has edited and contributed to manuscripts and books that have been published by Wiley, CUP, Nature, and Science.</p>
<p><b>International experts present innovative therapeutic strategies to treat cancer patients and prevent disease progression</b></p> <p><i>Extracellular Targeting of Cell Signaling in Cancer</i> highlights innovative therapeutic strategies to treat cancer metastasis and prevent tumor progression. Currently, there are no drugs available to treat or prevent metastatic cancer other than non-selective, toxic chemotherapy.  With contributions from an international panel of experts in the field, the book integrates diverse aspects of biochemistry, molecular biology, protein engineering, proteomics, cell biology, pharmacology, biophysics, structural biology, medicinal chemistry and drug development.</p> <p>A large class of proteins called kinases are enzymes required by cancer cells to grow, proliferate, and survive apoptosis (death) by the immune system. Two important kinases are MET and RON which are receptor tyrosine kinases (RTKs) that initiate cell signaling pathways outside the cell surface in response to extracellular ligands (growth factors.) Both kinases are oncogenes which are required by cancer cells to migrate away from the primary tumor, invade surrounding tissue and metastasize. MET and RON reside on both cancer cells and the support cells surrounding the tumor, called the microenvironment. MET and RON are activated by their particular ligands, the growth factors HGF and MSP, respectively. Blocking MET and RON kinase activation and downstream signaling is a promising therapeutic strategy for preventing tumor progression and metastasis. Written for cancer physicians and biologists as well as drug discovery and development teams in both industry and academia, this is the first book of its kind which explores novel approaches to inhibit MET and RON kinases other than traditional small molecule kinase inhibitors. These new strategies target key tumorigenic processes on the outside of the cell, such as growth factor activation by proteases. These unique strategies have promising potential as an improved alternative to kinase inhibitors, chemotherapy, or radiation treatment. </p>

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