<p>Contributors vii<br /> Preface xi</p> <p><b>Section I</b></p> <p><b>Diversity of CRISPR‐Cas Systems 1</b></p> <p>1 CRISPR-Cas Systems: Core Features and Common Mechanisms 3<br /> <i>Rodolphe Barrangou, Luciano A. Marraffini, and Erik J. Sontheimer</i></p> <p>2 Evolutionary Classification of CRISPR-Cas Systems 13<br /> <i>Kira S. Makarova, Yuri I. Wolf, and Eugene V. Koonin</i></p> <p>3 Molecular Mechanisms of Type I CRISPR-Cas Systems 39<br /> <i>John van der Oost</i></p> <p>4 Molecular Mechanisms of Type II CRISPR‐Cas Systems 53<br /> <i>Tautvydas Karvelis and Virginijus Siksnys</i></p> <p>5 Mechanism of Type III CRISPR‐Cas Immunity 71<br /> <i>Luciano A. Marraffini</i></p> <p>6 Type V CRISPR‐Cas Systems 85<br /> <i>Morgan Quinn Beckett, Anita Ramachandran, and Scott Bailey</i></p> <p>7 CRISPR-Cas13: Biology, Mechanism, and Applications of RNA-Guided, RNA-Targeting CRISPR Systems 99<br /> <i>Omar O. Abudayyeh and Jonathan S. Gootenberg </i></p> <p><b>Section II</b></p> <p><b>CRISPR‐Cas Biology 121</b></p> <p>8 CRISPR-Cas, Horizontal Gene Transfer, and the Flexible (Pan) Genome 123<br /> <i>Uri Gophna</i></p> <p>9 Evasion Tactics Manifested by Bacteriophages against Bacterial Immunity 131<br /> <i>Jenny Y. Zhang, Sutharsan Govindarajan, and Joseph Bondy-Denomy</i></p> <p>10 Regulation of CRISPR‐Cas Expression and Function 145<br /> <i>Leah M. Smith, Aroa Rey Campa, and Peter C. Fineran</i></p> <p><b>Section III</b></p> <p><b>CRISPR‐Based Technologies and Applications 163</b></p> <p>11 Genome Editing with CRISPR‐Cas Systems 165<br /> <i>Peter Lotfy and Patrick D. Hsu</i></p> <p>12 Genetic and Epigenetic Modulation of Gene Expression by CRISPR-dCas Systems 195<br /> <i>Jasprina N. Noordermeer, Crystal Chen, and Lei S. Qi</i></p> <p>13 CRISPR Screens 213<br /> <i>Jonathan D. D’Gama, Joseph S. Park, and Matthew K. Waldor</i></p> <p>14 CRISPR‐Based Antimicrobials 233<br /> <i>Justen Russell and David Bikard</i></p> <p>15 Exploiting CRISPR‐Cas Systems To Provide Phage Resistance in Industrial Bacteria 249<br /> <i>Rodolphe Barrangou and Avery Roberts</i></p> <p>16 Recording Biological Information with CRISPR‐Cas Systems 267<b><br /> </b><i>Mariia Y. Cherepkova, Tanmay Tanna, and Randall J. Platt</i></p> <p>Afterword 281</p> <p>Index 283</p>