Details

<p>Preface IX</p> <p>Preface to the 2nd edition X</p> <p>Introduction 1</p> <p><b>Introduction</b></p> <p>Early developments 2</p> <p>Biotechnology today 4</p> <p>Microbiology</p> <p>Viruses 6</p> <p>Bacteriophages 8</p> <p>Microorganisms 10</p> <p>Bacteria 12</p> <p>Yeasts 14</p> <p>Fungi 16</p> <p>Algae 18</p> <p>Some bacteria of importance for biotechnology 20</p> <p>Microorganisms: isolation, preservation, safety 22</p> <p>Microorganisms: strain improvement 24</p> <p><b>Biochemistry</b></p> <p>Biochemistry 26</p> <p>Amino acids, peptides, proteins 28</p> <p>Enzymes: structure, function, kinetics 30</p> <p>Sugars, glycosides, oligo- and polysaccharides 32</p> <p>Lipids, membranes, membrane proteins 34</p> <p>Metabolism 36</p> <p>Genetic engineering DNA: structure 38</p> <p>DNA: function 40</p> <p>RNA 42</p> <p>Genetic engineering: general steps 44</p> <p>Preparation of DNA 46</p> <p>Other useful enzymes for DNA manipulation 48</p> <p>PCR: general method 50</p> <p>PCR: laboratory methods 52</p> <p>DNA: synthesis and size determination 54</p> <p>DNA sequencing 56</p> <p>Transfer of foreign DNA in living cells (transformation) 58</p> <p>Gene cloning and identification 60</p> <p>Gene expression 62</p> <p>Gene silencing 64</p> <p>Epigenetics 66</p> <p>Gene libraries and gene mapping 68</p> <p>Genetic maps of prokaryotes 70</p> <p>Genetic maps of eukaryotes 72</p> <p>Metagenomics 74</p> <p><b>Cell biology</b></p> <p>Cell biology 76</p> <p>Stem cells 78</p> <p>Blood cells and immune system 80</p> <p>Antibodies 82</p> <p>Reporter groups 84</p> <p>Solid state fermentation (SSF) 86</p> <p>Growing microorganisms 88</p> <p>Growth kinetics and product formation 90</p> <p>Fed-batch, continuous and high cell density fermentation 92</p> <p>Fermentation technology 94</p> <p>Fermentation technology: scale-up 96</p> <p>Cultivation of mammalian cells 98</p> <p>Mammalian cell bioreactors 100</p> <p>Enzyme and cell reactors 102</p> <p>Recovery of bioproducts 104</p> <p>Recovery of proteins: chromatography 106</p> <p>Economic aspects of industrial processes 108</p> <p><b>Food and food additives</b></p> <p>Alcoholic beverages 110</p> <p>Beer 112</p> <p>Fermented food 114</p> <p>Food and lactic acid fermentation 116</p> <p>Prebiotics and probiotics 118</p> <p>Bakers’ yeast and fodder yeasts 120</p> <p>Fodder yeasts from petroleum feedstocks, single cell oil 122</p> <p>Amino acids 124</p> <p>l-Glutamic acid 126</p> <p>d,l-Methionine, l-lysine, and l-threonine 128</p> <p>Aspartame™, l-phenylalanine, and l-aspartic acid 130</p> <p>Amino acids via enzymatic transformation 132</p> <p>Vitamins 134</p> <p>Nucleosides and nucleotides 136</p> <p><b>Industrial products</b></p> <p>Bio-Ethanol 138</p> <p>1-Butanol 140</p> <p>Higher alcohols and alkenes 142</p> <p>Acetic acid / vinegar 144</p> <p>Citric acid 146</p> <p>Lactic acid, 3-hydroxy-propionic acid (3-HP) 148</p> <p>Gluconic acid and “green” sugar chemicals 150</p> <p>Dicarboxylic acids 152</p> <p>Biopolymers: Polyesters 154</p> <p>Biopolymers: Polyamides 156</p> <p>Polysaccharides 158</p> <p>Biosurfactants 160</p> <p>Fatty acids and their esters 162</p> <p><b>Enzyme technology</b></p> <p>Biotransformation 164</p> <p>Technical enzymes 166</p> <p>Applied enzyme catalysis 168</p> <p>Regio- and enantioselective enzymatic synthesis 170</p> <p>Enzymes as processing aids 172</p> <p>Detergent enzymes 174</p> <p>Enzymes for starch hydrolysis 176</p> <p>Enzymatic starch hydrolysis 178</p> <p>Enzymes and sweeteners 180</p> <p>Enzymes for the hydrolysis of cellulose and polyoses 182</p> <p>Enzymes in pulp and paper processing 184</p> <p>Pectinases 186</p> <p>Enzymes and milk products 188</p> <p>Enzymes in baking and meat processing 190</p> <p>Other enzymes for food products and animal feed 192</p> <p>Enzymes in leather and textile treatment 194</p> <p>Procedures for obtaining novel technical enzymes 196</p> <p>Protein design 198</p> <p><b>Antibiotics</b></p> <p>Antibiotics: occurrence, applications, mechanism of action 200</p> <p>Antibiotics: screening, industrial production, and mechanism of action 202</p> <p>Antibiotic resistance 204</p> <p>β-Lactam antibiotics: structure, biosynthesis, and mechanism of action 206</p> <p>β-Lactam antibiotics: manufacture 208</p> <p>Amino acid and peptide antibiotics 210</p> <p>Glycopeptide, lipopeptide, polyether, and nucleoside anti-biotics 212</p> <p>Aminoglycoside antibiotics 214</p> <p>Tetracyclines, chinones, chinolones, and other aromatic antibiotics 216</p> <p>Macrolide antibiotics 218</p> <p>New pathways to antibiotics 220</p> <p><b>Pharmaceuticals and medical technology</b></p> <p>Insulin 222</p> <p>Growth hormone and other hormones 224</p> <p>Hemoglobin, serum albumen, and lactoferrin 226</p> <p>Blood clotting agents 228</p> <p>Anticoagulants and thrombo-lytic agents 230</p> <p>Enzyme inhibitors 232</p> <p>Interferons 234</p> <p>Interleukins and “anti-interleukins” 236</p> <p>Erythropoietin and other growth factors 238</p> <p>Other therapeutic proteins 240</p> <p>Monoclonal and catalytic antibodies 242</p> <p>Recombinant antibodies 244</p> <p>Therapeutic antibodies 246</p> <p>Vaccines 248</p> <p>Recombinant vaccines 250</p> <p>Steroid biotransformations 252</p> <p>Diagnostic enzymes 254</p> <p>Enzyme tests 256</p> <p>Biosensors 258</p> <p>Immunoanalysis 260</p> <p>Glycobiology 262</p> <p><b>Agriculture and environment</b></p> <p>Animal breeding 264</p> <p>Embryo transfer, cloned animals 266</p> <p>Gene maps 268</p> <p>Transgenic animals 270</p> <p>Breeding, gene pharming and xenotransplantation 272</p> <p>Plant breeding 274</p> <p>Plant tissue surface culture 276</p> <p>Plant cell suspension culture 278</p> <p>Transgenic plants: methods 280</p> <p>Transgenic plants: resistance 282</p> <p>Transgenic plants: products 284</p> <p>Aerobic wastewater treatment 286</p> <p>Anaerobic wastewater and sludge treatment 288</p> <p>Biological treatment of exhaust air 290</p> <p>Biological soil treatment 292</p> <p>Microbial leaching, biofilms, and biocorrosion 294</p> <p><b>Megatrends</b></p> <p>The human genome 296</p> <p>Functional analysis of the human genome 298</p> <p>Pharmacogenomics, nutrigenomics 300</p> <p>DNA assays 302</p> <p>Gene therapy 304</p> <p>Induced pluripotent stem cells (iPS) 306</p> <p>Tissue Engineering 308</p> <p>Drug screening 310</p> <p>High-throughput sequencing 312</p> <p>Proteomics 314</p> <p>DNA and protein arrays 316</p> <p>Metabolic engineering 318</p> <p>Synthetic biology 320</p> <p>Systems biology 322</p> <p>Bioinformatics: sequence and structural databases 324</p> <p>Bioinformatics: functional analyses 326</p> <p>Carbon sources (C-sources) 328</p> <p>Biorefineries 330</p> <p>Safety in genetic engineering 332</p> <p>Regulation of products derived from biotechnology 334</p> <p>Ethical considerations and acceptance 336</p> <p>Patents in biotechnology 338</p> <p>International aspects of biotechnology 340</p> <p>Further Reading 343</p> <p>Index 376</p> <p>Picture Credits 398</p>

"...you should have it close at hand on your desktop as you read new articles. I would recommend this reference book...not only to clinical chemists, pathalogists, and medical technologists, but to anyone in medicine who wants to know how all the new therapeutic agents are made and appriciates the impact of biotechnology an medicine and society."<br> Clinical Chemistry<br> <br> "...this book is a useful, interesting and colourful guide to modern biotechnology and genetic engineering. It will achieve its objective of providing students with an overview of the field presented in manageable portions and a clear and accessible manner, but it will also be a source of information, a useful reference and an interesting read for any researcher who is working across the traditional boundaries of chemistry, biology or biochemistry."<br> ChemBioChem<br> <br> "This pocket guide can be recommended unreservedly for all students and researchers in natural and engineering sciences and medicine, but is also useful to readers with a general interest in biotechnology and genetic engineering. I can certainly agree with the final sentence of the book cover: A perfect introduction to the field - for professionals and students."<br> Angewandte Chemie IE<br> <br> Erwahnung in: Process<br> "...provides a broad coverage of the relevant facts on products, methods and applications in biotechnology and genetic engineering...Instructive yet attractive color illustrations and a didactic approach throughout the book..."<br> Process worldwide<br> <br> "Beginners and experts will like this wonderfully composed book."<br> Journal of Statistical Computation & Simulation<br> <br> "In the wilderness of biotechnology, Schmid's "Pocket Guide to Biotechnology and Genetic Engineering" is a welcome addition that with all likelihood will find many friends. During the review process it has definitely found one! The book is excellently produced. The figures are as sharp as tacks and as informative as the text...The overall verdict is: useful and recommendable to students and biotechnologists alike."<br> Engineering in Life Sciences

Rolf Schmid studied chemistry, biochemistry and microbiology in Munich and Freiburg (Germany). After postdoctoral training in Gif-sur-Yvette (France) and Austin (USA), he joined Henkel KGaA in Dusseldorf (Germany) where he worked for 15 years, eventually as Director R&D Biotechnology. In 1987, he was invited to direct the Institute for Enzyme Technology at Braunschweig Technical University and the Division of Enzyme Technology and Chemistry of Natural Products at the 'Gesellschaft fur biotechnologische Forschung' (GBF) in Braunschweig (Germany). From 1993 to 2009, he was Director of the Institute for Enzyme Technology at Stuttgart University (Germany). After having obtained an MBA diploma from the University of Reutlingen in 2007, he founded the consulting agency www.bio4business.eu in Stuttgart, followed in 2015 by the Steinbeis Consulting Agency www.asia-tech.eu. <br> <br> Dr. Claudia Schmidt-Dannert is Distinguished McKnight Professor in the Department of Biochemistry, Molecular Biology and Biophysics, at the University of Minnesota (USA). She studied Biology and Biochemistry at the Carolo Wilhelmina University Braunschweig (Germany), where she received her Ph.D. in Biochemistry in 1994. Schmidt-Dannert held a Postdoc position in Molecular Biotechnology at the University of Stuttgart (Germany) and became Professor at the University of Minnesota in 2010. Her research focuses on exploring and utilizing the metabolic machineries of plants and microorganisms to enable the discovery and synthesis of valuable microbial or plant-derived compounds in engineered microbial cells.<br>

SG