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Preface<br> Introduction to Optics and Photophysics (Rainer Heintzmann)<br> Principles of Light Microscopy (Ulrich Kubitscheck)<br> Fluorescence Microscopy (Jurek W. Dobrucki)<br> Fluorescence Labeling (G. Ulrich Nienhaus, Karin Nienhaus)<br> Confocal Microscopy (Nikolaus Naredi-Rainer, Jens Prescher, Achim Hartschuh, Don C. Lamb)<br> Fluorescence Photobleaching and Photoactivation Techniques (Reiner Peters)<br> Forster Resonance Energy Transfer and Fluorescence Lifetime Imaging (Fred S. Wouters)<br> Single molecule microscopy in the live sciences (Markus Axmann, Josef Madl, Gerhard J. Schutz)<br> Super-Resolution Microscopy Interference and Pattern Techniques (Gerrit Best, Roman Amberger, Christoph Cremer)<br> STED Microscopy (Trevis J. Gould, Patrina A. Pellet, Joerg Bewersdorf)<br> Appendix: Practical guide to optical alignment (Rainer Heintzmann)<br>

<p>“A companion website provides excellent material for anyone teaching a course on this topic.  Summing Up: Recommended.  Graduate students and researchers/faculty.”  (<i>Choice</i>, 1 January 2014)</p> <p>“This high-level textbook is best suited for advanced undergraduate and graduate students in the biosciences, and as a reference book for researchers in the field of high-performance microscopy.”  (<i>Optics & Photonics News</i>, 19 December 2013)</p>

Ulrich Kubitscheck is the department head of Biophysical Chemistry at the Institute of Physical and Theoretical Chemistry at the Rheinische Friedrich-Wilhelms-Universitat in Bonn, Germany. Having obtained his academic degrees from the University of Bremen, he spent his career working at The Weizmann Institute of Science and the Institute of Medical Physics and Biophysics of the Westfalische Wilhelms-Universitat Munster before taking up his present appointment at Bonn University. Professor Kubitscheck develops single molecule imaging techniques, has authored over 60 scientific publications and has extensive experience in teaching courses on physical chemistry, biophysics and quantitative microscopy.

<p>A comprehensive introduction to advanced fluorescence microscopy methods and their applications.</p> <p>This is the first title on the topic designed specifically to allow students and researchers with little background in physics to understand both microscopy basics and novel light microscopy techniques. The book is written by renowned experts and pioneers in the field with a rather intuitive than formal approach. It always keeps the nonexpert reader in mind, making even unavoidable complex theoretical concepts readily accessible. All commonly used methods are covered.</p> <p>A companion website with additional references, examples and video material makes this a valuable teaching resource:</p> <p><a href="http://www.wiley-vch.de/home/fluorescence_microscopy/">http://www.wiley-vch.de/home/fluorescence_microscopy/</a></p>

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