Details

Proteomics in Practice


Proteomics in Practice

A Guide to Successful Experimental Design
2nd Completely Revised Edition

von: Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker

106,99 €

Verlag: Wiley-Blackwell
Format: PDF
Veröffentl.: 08.09.2008
ISBN/EAN: 9783527622306
Sprache: englisch
Anzahl Seiten: 502

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Beschreibungen

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. <br> Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.<br> Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Preface<br> Foreword<br> Abbreviations<br> Glossary of terms<br> INTRODUCTION <br> History<br> Critical points<br> Hypothesis driven approach vs. High Throughput <br> Complementarities of workflows<br> Concept of experimental planning<br> Success stories, highlights of useful references<br> PART I: PROTEOMICS TECHNOLOGY<br> Electrophoresis<br> Liquid Chromatography<br> Mass spectrometry<br> Bioinformatics tools / data mining<br> Methods under development<br> PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS<br> Strategy where to start<br> Gel-based workflow<br> LC-based workflow<br> Latest trends<br> Validation with Western blotting<br> Statistic evaluation<br> PART III: TROUBLE SHOOTING<br> Gel-based workflow<br> LC based workflow<br> References<br> Index<br>
"This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended." (<i>Internet Journal of Genomics and Proteomics</i> 2008)
Reiner Westermeier gained his doctorate in 1981 at the Technical University of Munich, Germany. There he remained engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours.<br> From 1984 to 1987 Reiner Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech, later GE Healthcare since July 1997, and its Senior Scientist for Proteomics since January 2002. <br> Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.<br> Tom Naven and Hans-Rudolf Hopker are also working for GE Healthcare as proteomics scientists.
A thoroughly revised edition of the successful practical guide to experimental methods in proteomics, which now includes DIGE technology and liquid chromatography.<br> <br> The first part, written in a textbook style, presents the various techniques and routes required in approaching proteomic analysis. Key methods explained are electrophoretic techniques including isoelectric focusing, SDS page, 2-D page and DIGE, and liquid chromatography techniques including ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques such as MALDI, ESR and FT ICR are addressed as well as a section on proteome functionality. The second part is a comprehensive, laboratory manual spanning the full range of methods from sample preparation to protein identification. The third part is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further information.<br> <br> Updates of protocols as well as animations illustrating crucial methodological steps are available on the Web at http://www.wiley-vch.de/books/info/978-3-527-31941-1/index.html<br> <br>

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