Details

HPLC Made to Measure

A Practical Handbook for Optimization
1. Aufl.

von: Stavros Kromidas
165,99 €
Verlag:	Wiley-VCH (D)
Format:	PDF
Veröffentl.:	05.06.2008
ISBN/EAN:	9783527611997
Sprache:	englisch
Anzahl Seiten:	786

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Beschreibungen

Titelbeschreibung

The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.

Inhaltsverzeichnis

ESSENTIAL ASPECTS OF OPTIMIZATION Principles of HPLC Optimization Illustrated by RP-Chromatography Fast Gradient Separations pH and Selectivity in Reversed-Phase Chromatography Selecting the Correct pH-Value for HPLC Optimization of Data Evaluation in Chromatography Calibration Characteristics and Uncertainty indicating Starting-Points for Method Optimization OPTIMIZATION IN DIFFERENT LC-MODI Comparison and Choice of Columns for RP-HPLC Column Selectivity in Reversed-Phase Chromatography The Use of Principal Component Analysis for the Characterisation of Reversed-Phase Liquid Chromatographic Stationary Phases Chemometrics - A Powerful Tool for Handling a Large Number of Data Linear Free Energy Relations - Tools for Column Characterization and Method Optimization in HPLC? Column Selectivity in Reversed-Phase Liquid Chromatography Understanding Selectivity by Use of Suspended-State High Resolution Magic Angle Spinning NMR Spectroscopy Optimization in Normal-Phase HPLC Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection Gel Filtration - Size Exclusion Chromatography of biopolymers Optimization in Affinity Chromatography Optimization of Chiral Separations in HPLC ?LC/nano LC - Optimization and Troubleshooting Microchip-Based Liquid Chromatography - Techniques and Possibilities Ultra-Performance Liquid Chromatography HYPHENATED TECHNIQUES Immunochromatographic Techniques Enhanced Characterization and Comprehensive Analyses by 2-dimensional Chromatography LC/MS - Hints and Recommendations for Optimization and Troubleshooting LC-NMR-Coupling COMPUTER-AIDED OPTIMIZATION Computer-Facilitated HPLC Method Development Using DryLab Software ChromSword Software for Automated and Computer-Assisted Development of HPLC Methods Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC USER REPORTS NanoLC-MS/MS in Proteomics Ways for the Verification of the Robustness in RP-HPLC Separation of Complex Sample Mixtures Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC-UV/MS/CLND

Rezension

"A valuable text by any estimation, I would recommend it highly for hands-on users or managers directing a group of chromatographers toward more efficient and effective HPLC separations." (Journal of the American Chemical Society, February 21, 2007)

Autorenportrait

Following his PhD thesis in 1983, Stavros Kromidas worked as a sales manager for Waters Chromatography . From 1989 to 2001 he was Managing Director of Novia GmbH, a consultancy company for analytical chemistry, and he has been an independent consultant since then. He has been working in the field of HPLC since 1978 and has given lectures and training courses since 1984. He is the author and co-author of numerous articles and several successful books.

Back cover copy

Alongside developing new ones, the optimization of existing methods is a key task in the HPLC laboratory. And a task that nowadays has to be solved increasingly fast and cost-efficiently. This handbook provides well-founded assistance in better meeting this challenge. Internationally renowned authors cover both the general basics and strategies in optimization, as well as the specific aspects involved in such different methods as RP-HPLC, NP-HPLC, micro- and nano-HPLC, and hyphenated techniques, such as LC/MS. Nor are such topics as column selection and chiral separations left uncovered. Some of the contributions present applications using common optimization software, such as DryLab or ChromSword. All of the chapters concentrate on the essentials and are written in a practically oriented style, while their self-contained structure allows for targeted references. Throughout the text, the authors offer concrete, practical tips as well as pertinent background information, together with insights into the optimization methods of seven major international companies from various sectors. The whole is rounded off with real-life reports from experienced users coming from the different areas of application, in particular the life sciences, such as proteomics or drug development.