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<p>List of Contributors xvii</p> <p>Preface xxi</p> <p>Acknowledgment xxv</p> <p><b>Section 1 Fundamentals of Charged Aerosol Detection 1</b></p> <p>1 Principles of Charged Aerosol Detection 3<br /><i>Paul H. Gamache and Stanley L. Kaufman</i></p> <p>1.1 Summary 3</p> <p>1.2 History and Introduction to the Technology 4</p> <p>1.3 Charged Aerosol Detection Process 9</p> <p>1.4 CAD Response Model 31</p> <p>1.5 Performance Characteristics 40</p> <p>2 Charged Aerosol Detection: A Literature Review 67<br /><i>Ian N. Acworth and William Kopaciewicz</i></p> <p>2.1 Introduction 67</p> <p>2.2 CAD History and Background 74</p> <p>2.3 Application Areas 79</p> <p>3 Practical Use of CAD: Achieving Optimal Performance 163<br /><i>Bruce Bailey, Marc Plante, David Thomas, Chris Crafts,and Paul H. Gamache</i></p> <p>3.1 Summary 163</p> <p>3.2 Introduction 164</p> <p>3.3 Factors Influencing CAD Performance 168</p> <p>3.4 System Configurations 177</p> <p>3.5 Method Transfer 180</p> <p>3.6 Calibration and Sensitivity Limits 182</p> <p>4 Aerosol?]Based Detectors in Liquid Chromatography: Approaches Toward Universal Detection and to Global Analysis 191<br /><i>Joseph P. Hutchinson, Greg W. Dicinoski, and Paul R. Haddad</i></p> <p>4.1 Summary 191</p> <p>4.2 Introduction 192</p> <p>4.3 Universal Detection Methods 194</p> <p>4.4 Factors Affecting the Response in Charged Aerosol Detection 198</p> <p>4.5 Gradient Compensation 204</p> <p>4.6 Response Models 205</p> <p>4.7 Green Chemistry 206</p> <p>4.8 Temperature Gradient Separations 209</p> <p>4.9 Supercritical CO2 Separations 210</p> <p>4.10 Capillary Separations 211</p> <p>4.11 Global Analysis and Multidimensional Separations 212</p> <p>4.12 Conclusions 215</p> <p><b>Section 2 Charged Aerosol Detection of Specific Analyte Classes 221</b></p> <p>5 Lipid Analysis with the Corona CAD 223<br /><i>Danielle Libong, Sylvie Heron, Alain Tchapla, and Pierre Chaminade</i></p> <p>5.1 Introduction 223</p> <p>5.2 Principles of Chromatographic Separation of Lipids 227</p> <p>5.3 Application: Strategy of Lipid Separation 235</p> <p>5.4 Literature Review: Early Use of Corona CAD in Lipid Analysis 257</p> <p>5.5 Calibration Strategies 264</p> <p>6 Inorganic and Organic Ions 289<br /><i>Xiaodong Liu, Christopher A. Pohl, and Ke Zhang</i></p> <p>6.1 Introduction 289</p> <p>6.2 Technical Considerations 291</p> <p>6.3 Applications 300</p> <p>7 Determination of Carbohydrates Using Liquid Chromatography with Charged Aerosol Detection 311<br /><i>Jeffrey S. Rohrer and Shinichi Kitamura</i></p> <p>7.1 Summary 311</p> <p>7.2 Liquid Chromatography of Carbohydrates 312</p> <p>7.3 Charged Aerosol Detection 314</p> <p>7.4 Why LC?]CAD for Carbohydrate Analysis? 315</p> <p>7.5 Early Applications of CAD to Carbohydrate Analysis 316</p> <p>7.6 Additional Applications of CAD to Carbohydrate Analysis 317</p> <p>8 Polymers and Surfactants 327<br /><i>Dawen Kou, Gerald Manius, Hung Tian, and Hitesh P. Chokshi</i></p> <p>8.1 Summary 327</p> <p>8.2 Introduction 328</p> <p>8.3 Polymer Analysis 328</p> <p>8.4 Polyethylene Glycol 329</p> <p>8.5 Surfactants336</p> <p>9 Application of Charged Aerosol Detection in Traditional Herbal Medicines 341<br /><i>Lijuan Liang, Yong Jiang, and Pengfei Tu</i></p> <p>9.1 Summary 341</p> <p>9.2 Introduction 342</p> <p>9.3 Factors that Affect the Sensitivity of CAD 343</p> <p>9.4 Application of CAD in Quality Analysis of Traditional Herbal Medicines 345</p> <p>9.5 Conclusion 353</p> <p>References 353</p> <p><b>Section 3 Industrial Applications of Charged Aerosol Detection 355</b></p> <p>10 Charged Aerosol Detection in Pharmaceutical Analysis: An Overview 357<br /><i>Michael Swartz, Mark Emanuele, and Amber Awad</i></p> <p>10.1 Summary 357</p> <p>10.2 Introduction 358</p> <p>10.3 Analytical Method Development 359</p> <p>10.4 Analytical Method Validation 361</p> <p>10.5 CAD in Analytical Method Transfer 363</p> <p>10.6 CAD in Formulation Development and Ion Analysis 364</p> <p>10.7 Carbohydrate Analysis by CAD 368</p> <p>10.8 CAD in Stability Analyses 371</p> <p>10.9 Conclusion 373</p> <p>References 374</p> <p>11 Impurity Control in Topiramate with High Performance Liquid Chromatography: Validation and Comparison of the Performance of Evaporative Light Scattering Detection and Charged Aerosol Detection 379<br /><i>David Ilko, Robert C. Neugebauer, Sophie Brossard, Stefan Almeling, Michael Turck, and Ulrike Holzgrabe</i></p> <p>11.1 Summary 379</p> <p>11.2 Introduction 380</p> <p>11.3 Material and Methods 382</p> <p>11.4 Results and Discussion 383</p> <p>11.5 Conclusion 390</p> <p>12 Applying Charged Aerosol Detection to Aminoglycosides: Development and Validation of an RP?]HPLC Method for Gentamicin and Netilmicin 393<br /><i>Arul Joseph and Abu Rustum</i></p> <p>12.1 Introduction 393</p> <p>12.2 Development and Validation of an RP?]HPLC Method for Gentamicin Using Charged Aerosol Detection 395</p> <p>12.3 Application of Strategy to Netilmicin Sulfate 410</p> <p>12.4 Conclusion 420</p> <p>Acknowledgments 420</p> <p>References 420</p> <p>13 Determination of Quaternary Ammonium Muscle Relaxants with Their Impurities in Pharmaceutical Preparations by LC?]CAD 425<br /><i>Agata Blazewicz, Magdalena Poplawska, Malgorzata Warowna?-Grzeskiewicz, Katarzyna Sarna, and Zbigniew Fijalek</i></p> <p>13.1 Summary 425</p> <p>13.2 Introduction 426</p> <p>13.3 Experimental 429</p> <p>13.4 Results and Discussion 431</p> <p>13.5 Conclusion 445</p> <p>Acknowledgments 445</p> <p>References 446</p> <p>14 Charged Aerosol Detection of Scale Inhibiting Polymers in Oilfield Chemistry Applications 449<br /><i>Alan K. Thompson</i></p> <p>14.1 Summary 449</p> <p>14.2 Background to Scale Inhibition in Oilfields 450</p> <p>14.3 Historical Methods of Analysis 455</p> <p>14.4 Charged Aerosol Detection for Polymeric Scale Inhibitors 459</p> <p>14.5 Conclusions and Further Work 468</p> <p>References 469</p> <p>15 Applications of Charged Aerosol Detection for Characterization of Industrial Polymers 471<br /><i>Paul Cools and Ton Brooijmans</i></p> <p>15.1 Introduction 471</p> <p>15.2 Liquid Chromatography of Polymers 472</p> <p>15.3 Solvents 475</p> <p>15.4 Quantitative Detection of Polymer Molecules 476</p> <p>15.5 Size Exclusion Chromatography and Charged Aerosol Detection 479</p> <p>15.6 Gradient Polymer Elution Chromatography and CAD 486</p> <p>15.7 Liquid Chromatography Combined with UV, CAD, and MS Detection 490</p> <p>15.8 Typical Examples of Industrial Applications Using LC?-MS?-CAD 492</p> <p>15.9 Epilogue 497</p> <p>Acknowledgments 497</p> <p>References 497</p> <p>Index 501</p>

<p><b> PAUL H. GAMACHE</b> is Director of Research and Development at Thermo Fisher Scientific. He has more than thirty years' experience within the analytical instrument industry. His primary area of expertise is in the development of instrumentation and techniques based on liquid chromatography. He has published more than 50 articles and book chapters including the first publication describing commercial CAD technology. In 2005 he was co-awardee of an NIH Metabolomics Roadmap research grant.

<p><b> The first book devoted exclusively to a highly popular, relatively new detection technique </b> <p><i> Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques</i> presents a comprehensive review of CAD theory, describes its advantages and limitations, and offers extremely well-informed recommendations for its practical use. Using numerous real-world examples based on contributors' professional experiences, it provides priceless insights into the actual and potential applications of CAD across a wide range of industries. <p> Charged aerosol detection can be combined with a variety of separation techniques and in numerous configurations. While it has been widely adapted for an array of industrial and research applications with great success, it is still a relatively new technique, and its fundamental performance characteristics are not yet fully understood. This book is intended as a tool for scientists seeking to identify the most effective and efficient uses of charged aerosol detection for a given application. Moving naturally from basic to advanced topics, the author relates fundamental principles, practical uses, and applications across a range of industrial settings, including pharmaceuticals, petrochemicals, biotech, and more. <ul> <li>Offers timely, authoritative coverage of the theory, experimental techniques, and end-user applications of charged aerosol detection </li> <li>Includes contributions from experts from various fields of applications who explore CAD's advantages over traditional HPLC techniques, as well as its limitations</li> <li>Provides a current theoretical and practical understanding of CAD, derived from authorities on aerosol technology and separation sciences</li> <li>Features numerous real-world examples that help relate fundamental properties and general operational variables of CAD to its performance in a variety of conditions</li> </ul> <br> <p><i> Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques</i> is a valuable resource for scientists who use chromatographic techniques in academic research and across an array of industrial settings, including the biopharmaceutical, biotechnology, biofuel, chemical, environmental, and food and beverage industries, among others.

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