Details

Protein Carbonylation

Principles, Analysis, and Biological Implications
1. Aufl.

von: Joaquim Ros
153,99 €
Verlag:	Wiley
Format:	EPUB
Veröffentl.:	15.05.2017
ISBN/EAN:	9781119374954
Sprache:	englisch
Anzahl Seiten:	416

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Beschreibungen

Titelbeschreibung

Protein carbonylation has attracted the interest of a great number of laboratories since the pioneering studies at the Earl Stadtman’s lab at NIH started in early 1980s. Since then, detecting protein carbonyls in oxidative stress situations became a highly efficient tool to uncover biomarkers of oxidative damage in normal and altered cell physiology. In this book, research groups from several areas of interest have contributed to update the knowledge regarding detection, analyses and identification of carbonylated proteins and the sites where these modifications occur. The scientific community will benefit from these reviews since they deal with specific, detailed technical approaches to study formation and detection of protein carbonyls. Moreover, the biological impact of such modifications in metabolic, physiologic and structural functions and, how these alterations can help understanding the downstream effects on cell function are discussed. <ul> <li>Oxidative stress occurs in all living organisms and affects proteins and other macromolecules: Protein carbonylation is a measure of oxidative stress in biological systems</li> <li>Mass spectrometry, fluorescent labelling, antibody based detection, biotinylated protein selection and other methods for detecting protein carbonyls and modification sites in proteins are described</li> <li>Aging, neurodegenerative diseases, obstructive pulmonary diseases, malaria, cigarette smoke, adipose tissue and its relationship with protein carbonylation</li> <li>Direct oxidation, glycoxidation and modifications by lipid peroxidation products as protein carbonylation pathways</li> <li>Emerging methods for characterizing carbonylated protein networks and affected metabolic pathways</li> </ul>

Inhaltsverzeichnis

List of Contributors xii Preface xvi 1 Reactive Oxygen Species Signaling from the Perspective of the Stem Cell 1 Saghi Ghaffari and Raymond Liang 1.1 Introduction 1 1.2 ROS Regulation 2 1.3 ROS Signaling 3 1.4 ROS and Stem Cells 5 1.5 ROS, Metabolism, and Epigenetic Influence 9 1.6 Stem Cells and Mitochondria 9 1.7 ROS and Stem Cell Aging 12 2 Analysis of Protein Carbonylation 24 Ashraf G. Madian, Fred E. Regnier, and Ao Zeng 2.1 Introduction 24 2.2 In Vivo Carbonylation Reactions 27 2.3 Analytical Derivatization of Carbonylated Groups 34 2.4 Selective Purification and/or Detection of Carbonylated Proteins and Peptides 36 2.5 Oxidative Stress?]Based PTMS Not Involving Carbonylation 38 3 Diversity of Protein Carbonylation Pathways: Direct Oxidation,Glycoxidation, and Modifications by Lipid Peroxidation Products 48 Maria Fedorova 3.1 Introduction 48 3.2 Pathways of Protein Carbonylation 49 3.3 Analytical Methods for Detection of Total and Specific Protein Carbonylation 57 3.4 Protein Susceptibility to Different Carbonylation Pathways and Modifications Cross?]Talk 67 4 Protein Carbonylation by Reactive Lipids 83 Koji Uchida 4.1 Introduction 83 4.2 Chemistry of Protein Carbonylation by Reactive Lipid Aldehydes 84 4.3 Antigenicity of Protein Carbonyls 87 4.4 Thiolation of Protein Carbonyls 89 4.5 Reductive Amination?]Based Fluorescent Labeling of Protein Carbonyls 91 5 Mechanism and Functions of Protein Decarbonylation 97 Yuichiro J. Suzuki 5.1 Protein Carbonylation 97 5.2 Primary Protein Carbonylation in Cell Signaling 98 5.3 Discovery and Mechanisms of Protein Decarbonylation 101 5.4 Proposed Functions of Protein Decarbonylation in Oxidative Stress and Redox Signaling 103 6 Carbonylated Proteins and Their Metabolic Regulation: Overview of Mechanisms, Target Proteins, and Characterization Using Proteomic Methods 110 Somaieh Afiuni?]Zadeh and Timothy J. Griffin 6.1 Metabolic Regulation and Reactive Oxygen Species 110 6.2 ROS and Protein Carbonylation 111 6.3 Metabolic Control and Characteristics of Carbonylated Proteins 113 6.4 Protein Targets of Carbonylation and Implications in Human Health 114 6.5 Technologies and Methods for Characterizing Protein Carbonylation 118 6.6 Emerging Multifunctional Reagents for Protein Carbonylation Analysis via MS 119 6.7 Emerging Methods for Characterizing Carbonylated Protein Networks and Affected Pathways 123 7 Oxidative Stress and Protein Carbonylation in Malaria 131 Maria Linares, Antonio Puyet, Amalia Diez, and Jose M. Bautista 7.1 Introduction 131 7.2 Oxidative Stress during Malaria Infection 132 7.3 Protein Carbonylation in Plasmodium and Oxidative Targeting of Antimalarials 137 7.4 Oxidative Dysfunction in Host Tissues 143 7.5 Host Tolerance to Malaria by Modulation of Oxidative Stress Responses 148 7.6 Perspectives 153 8 Protein Carbonylation in Brains of Subjects with Selected Neurodegenerative Disorders 167 Tanea T. Reed and D. Allan Butterfield 8.1 Introduction to Protein Carbonylation 167 8.2 Relationship between ROS and Oxidative Stress 169 8.3 An Overview of Some Neurodegenerative Diseases 171 8.4 Role of Protein Carbonylation in Brains of Subjects with AD 174 8.5 An Introduction to Tauopathies 185 8.6 An Introduction to Amyotrophic Lateral Sclerosis 186 9 Cigarette Smoke?]Induced Protein Carbonylation: Focus on Recent Human Studies 206 Graziano Colombo, Maria Lisa Garavaglia, Aldo Milzani, and Isabella Dalle?]Donne 9.1 Introduction 206 9.2 Protein Carbonylation in Human Smokers 212 9.3 Protein Carbonylation in Cultured Human Cell Models of Exposure to CS 218 9.4 Limitations and Congruence of In Vivo and In Vitro Human Studies 228 10 Chronic Obstructive Pulmonary Disease and Oxidative Damage 241 Esther Barreiro 10.1 Introduction 242 10.2 Protein Oxidation in Tissues 244 10.3 Antioxidants in Skeletal Muscle Fibers 247 10.4 Implications of Protein Carbonylation in COPD Skeletal Muscle Dysfunction 249 10.5 Muscle Protein Carbonylation and Exercise in COPD Patients 252 10.6 Protein Carbonylation in Muscles Exposed to Chronic Cigarette Smoke 253 10.7 Protein Carbonylation in Cancer Cachexia Models 255 10.8 Protein Carbonylation as a Predisposing Mechanism of Lung Cancer in COPD 257 11 Protein Carbonylation in Aging and Senescence 272 Jeannette Konig, Tobias Jung and Tilman Grune 11.1 Introduction 272 11.2 Protein Oxidation during Aging 274 11.3 Chemistry of Protein Carbonylation and Fate of Carbonylated Proteins 277 11.4 Protein Carbonyls in Cellular Aging Models 279 11.5 Protein Carbonylation in Aging Organisms 280 12 Adipose Carbonylation and Mitochondrial Dysfunction 291 Amy K. Hauck, Dalay H. Olson, Joel S. Burrill, and David A. Bernlohr 12.1 Introduction 291 12.2 Reactive Oxygen Species (ROS) 292 12.3 Oxidative Stress and Obesity 298 12.4 Detection of Protein Carbonylation 303 12.5 Outcomes of Protein Carbonylation 306 13 Protein Carbonylation in Plants 321 Ian Max Moller, Jesper F. Havelund, and Adelina Rogowska?]Wrzesinska 13.1 Introduction 322 13.2 Turnover of Reactive Oxygen Species in Plants 323 13.3 Methods Used in Plants for Quantifying and Identifying Carbonylation Sites 325 13.4 Protein Carbonylation in Plants 326 13.5 Protein Carbonylation in Plant Mitochondria 328 13.6 Protein Carbonylation in Seeds 333 14 Specificity of Protein Carbonylation and Its Relevance in Aging 340 Elisa Cabiscol, Jordi Tamarit, and Joaquim Ros 14.1 Introduction 340 14.2 Specificity of Protein Oxidative Damage 341 14.3 Protein Carbonylation in Aging 348 Index 384

Autorenportrait

Joaquim Ros is Professor at the University of Lleida. From 1995 his research interest has been focused on studying the effect of oxidative stress on proteins in several models (from bacteria to humans) and how this damage affects protein function. He is the head of the Dept. of Basic Medical Sciences, Faculty of Medicine, University of Lleida.

Back cover copy

Written by worldwide experts in the field this book covers several areas of interests in the topic of Protein Carbonylation and gives expert advice on methodology for the analysis of protein oxidative modification, with an emphasis on mass spectrometry. It covers the modern methodology for the detection, quantitation, and localization of carbonylation site. <ul> <li>Oxidative stress occurs in all living organisms and affects proteins and other macromolecules: Protein carbonylation is a measure of oxidative stress in biological systems</li> <li>Mass spectrometry, fluorescent labelling, antibody based detection, biotinylated protein selection and other methods for detecting protein carbonyls and modification sites in proteins are described</li> <li>Aging, neurodegenerative diseases, obstructive pulmonary diseases, malaria, cigarette smoke, adipose tissue and its relationship with protein carbonylation</li> <li>Direct oxidation, glycoxidation and modifications by lipid peroxidation products as protein carbonylation pathways</li> <li>Emerging methods for characterizing carbonylated protein networks and affected metabolic pathways</li> </ul> The book will appeal to scientists in biomedical, pharmaceutical and medical institutes. Given that most of the contributors are involved in academic institutions all over the world, the book will serve as a reference text for lecturers and as a handbook for graduate and undergraduate students in the fields of biological, biomedical, medical, pharmaceutical sciences. Protein carbonylation has attracted the interest of a great number of laboratories since the pioneering studies at the Earl Stadtman???s lab at NIH started in early 1980s. Since then, detecting protein carbonyls in oxidative stress situations became a highly efficient tool to uncover biomarkers of oxidative damage in normal and altered cell physiology. In this book, research groups from several areas of interest have contributed to update the knowledge regarding detection, analyses and identification of carbonylated proteins and the sites where these modifications occur. The scientific community will benefit from these reviews since they deal with specific, detailed technical approaches to study formation and detection of protein carbonyls. Moreover, the biological impact of such modifications in metabolic, physiologic and structural functions and, how these alterations can help understanding the downstream effects on cell function are discussed.