Details

<p>List of Figures, xix</p> <p>List of Color Plates, xxiii</p> <p>List of Tables, xxv</p> <p>List of Protocols, xxvii</p> <p>List of Minireviews, xxix</p> <p>Preface and Acknlowledgments, xxxi</p> <p>Abbreviations, xxxiii</p> <p>About the Companion Website xxxviii</p> <p>1. Introduction, 1</p> <p>2. Biology of Cultured Cells, 19</p> <p>3. Laboratory Design and Layout, 37</p> <p>4. Equipment and Materials, 51</p> <p>5. Aseptic Technique, 73</p> <p>6. Safety, Bioethics, and Validation, 89</p> <p>7. Culture Vessels and Substrates, 111</p> <p>8. Defined Media and Supplements, 125</p> <p>9. Serum-Free Media, 149</p> <p>10. Preparation and Sterilization, 173</p> <p>10. Supplementary Material*</p> <p>11. Primary Culture, 207</p> <p>11. Supplementary Material*</p> <p>12. Subculture and Cell Lines, 235</p> <p>13. Authentication and Validation, 259</p> <p>14. Microbial Contamination, 289</p> <p>15. Cryopreservation and Banking, 307</p> <p>16. Cloning and Selection, 327</p> <p>16. Supplementary Material*</p> <p>17. Cell Sorting, 347</p> <p>18. Cell Line Characterization, 359</p> <p>19. Differentiation, 383</p> <p>20. Three?]Dimensional Culture 401</p> <p>21. Scale?]up and Automation, 427</p> <p>22. Senescence, Immortalization, and Transformation, 455</p> <p>23. Quantitation, 483</p> <p>23 Supplementary Material*</p> <p>24. Cytotoxicity, 513</p> <p>24. Supplementary Material*</p> <p>25. Culture of Specific Cell Types, 531</p> <p>25. Supplementary Material*</p> <p>26. Stem Cells, 571</p> <p>26. Supplementary Material*</p> <p>27. Training Programs, 591</p> <p>28. Problem Solving 627</p> <p>29. In Conclusion, 651</p> <p>Supplementary Material*</p> <p>Appendix III: Sources of Equipment and Material</p> <p>Appendix IV: Suppliers and Other Resource</p>

<p><b>R. IAN FRESHNEY,</b> PhD, is an honorary Senior Research Fellow at the Instituteof Cancer Sciences at the University of Glasgow in Glasgow, UK. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

<p>Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made–particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. <i>Culture</i> <i>of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition</i> is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. <p>This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to limit the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. <p><i>Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition</i>, provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees, and technicians. <ul> <li>Includes clearly understood protocols, lists the requisite materials for all basic procedures, and provides guidelines on safety and bioethics</li> <li>Specialized procedures explained in similar detail to allow use without further supplementation from the</li> primary literature</li> <li>Includes additional series of minireviews on topics such as stem cells and regenerative medicine, 3D culture, high throughput screening, and authentication assays</li> <li>Supplementary material with descriptive diagrams and photographs</li> <li>Contains instructions on preparing and equipping the cell culture laboratory, including preparation and sterilization of media, and reagents, with lists of materials and equipment sources</li> <li>Contains a training chapter with detailed training exercises, some with experimental components</li> <li>Provides cautionary advice on instability, contamination, and misidentification with appropriate protocols to enable correct quality control</li> </ul>

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